Rose Bengal Chloramphenicol Agar  (Synonyms: Rose Bengal Medium)

Rose-Bengal Chloramphenicol Agar is a selective medium for the enumeration of yeasts and moulds from a wide variety of foodstuffs. The medium has a neutral pH and chloramphenicol is used as a selective agent to suppress the growth of bacteria^[1].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Ingredients:
Peptone: 5.0 g/L;
Glucose: 10.0 g/L;
Potassium dihydrogen phosphate: 1.0 g/L;
Magnesium sulfate (anhydrous): 0.5 g/L;
Agar: 20.0 g/L;
Rose Bengal Chloramphenicol Agar: 0.033 g/L;
Chloramphenicol: 0.1 g/L

Usage:
Weigh 36.6 g of this product, heat and boil to dissolve in 1000 mL of distilled water, divide into Erlenmeyer bottles, sterilize at 121°C for 15 minutes, and set aside.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Solid

Off-white to light brown

[Rose Bengal Chloramphenicol Agar]

Room temperature in continental US; may vary elsewhere.

RT, protect from light

• [1]. Bengal R. Rose bengal chloramphenicol (RBC) agar. Progress in Industrial Microbiology, 1995, 34: 431-433.