Search Result

Pathways Recommended:	Metabolic Enzyme/Protease

Results for "

NS3 protease

" in MedChemExpress (MCE) Product Catalog:

By Targets:	HCV Protease (52) Ser/Thr Protease (1) Virus Protease (28) Akt (1) Amyloid-β (1) Apoptosis (5) Aurora Kinase (4) Bacterial (6) BCRP (1) Casein Kinase (1) COX (1) Cytochrome P450 (2) Dengue Virus (11) DNA/RNA Synthesis (7) Drug Derivative (1) Endogenous Metabolite (1) ERK (1) Flavivirus (13) Fluorescent Dye (1) Fungal (1) G-quadruplex (2) Glucocorticoid Receptor (2) Glucose-6-Phosphate Isomerase (GPI) (1) HBV (1) HCV (38) HIV (1) HSV (1) Indoleamine 2,3-Dioxygenase (IDO) (4) Influenza Virus (1) Isotope-Labeled Compounds (4) mAChR (4) mTOR (1) P-glycoprotein (1) Parasite (4) PGE synthase (1) PI3K (1) Ras (1) Reactive Oxygen Species (ROS) (1) Reference Standards (9) SARS-CoV (26) Src (2) STAT (1) Thrombin (1) Toll-like Receptor (TLR) (1) γ-secretase (1)
By Research Areas:	Cancer (11) Infection (85) Inflammation/Immunology (10) Metabolic Disease (4) Neurological Disease (6)

By Targets:

HCV Protease (52)

Ser/Thr Protease (1)

Virus Protease (28)

Akt (1)

Amyloid-β (1)

Apoptosis (5)

Aurora Kinase (4)

Bacterial (6)

BCRP (1)

Casein Kinase (1)

COX (1)

Cytochrome P450 (2)

Dengue Virus (11)

DNA/RNA Synthesis (7)

Drug Derivative (1)

Endogenous Metabolite (1)

ERK (1)

Flavivirus (13)

Fluorescent Dye (1)

Fungal (1)

G-quadruplex (2)

Glucocorticoid Receptor (2)

Glucose-6-Phosphate Isomerase (GPI) (1)

HBV (1)

HCV (38)

HIV (1)

HSV (1)

Indoleamine 2,3-Dioxygenase (IDO) (4)

Influenza Virus (1)

Isotope-Labeled Compounds (4)

mAChR (4)

mTOR (1)

P-glycoprotein (1)

Parasite (4)

PGE synthase (1)

PI3K (1)

Ras (1)

Reactive Oxygen Species (ROS) (1)

Reference Standards (9)

SARS-CoV (26)

Src (2)

STAT (1)

Thrombin (1)

Toll-like Receptor (TLR) (1)

γ-secretase (1)

By Research Areas:

Cancer (11)

Infection (85)

Inflammation/Immunology (10)

Metabolic Disease (4)

Neurological Disease (6)

Inhibitors & Agonists

Fluorescent Dyes

Biochemical Assay Reagents

Peptides

Natural
Products

Recombinant Proteins

Isotope-Labeled Compounds

Glecaprevir 5+ Cited Publications ABT-493	HCV HCV Protease SARS-CoV	Infection
Glecaprevir is a novel HCV NS3/4A protease inhibitor, with IC₅₀ values ranging from 3.5 to 11.3 nM. Glecaprevir is also a SARS-CoV 3CL ^pro inhibitor with an IC₅₀ of 4.09 μM .

Asunaprevir 40+ Cited Publications BMS-650032	HCV HCV Protease SARS-CoV	Infection
Asunaprevir (BMS-650032) is a potent and orally bioavailable hepatitis C virus (HCV) NS3 protease inhibitor, with IC₅₀ of 0.2 nM-3.5 nM . Asunaprevir inhibits SARS-CoV-2 3CL ^pro activity .

Telaprevir 40+ Cited Publications VX-950	HCV Protease HCV SARS-CoV	Infection
Telaprevir (VX-950) is a highly selective, reversible, and potent peptidomimetic inhibitor of the HCV NS3-4A protease, the steady-state inhibitory constant (K_i) of Telaprevir is 7 nM against a genotype 1 (H strain) NS3 protease domain plus a NS4A cofactor peptide . Telaprevir inhibits SARS-CoV-2 3CL ^pro activity .

Simeprevir Maximum Cited Publications 45 Publications Verification TMC435; TMC435350	HCV HCV Protease SARS-CoV DNA/RNA Synthesis	Infection
Simeprevir (TMC435; TMC435350) is an oral, potent and highly specific hepatitis C virus (HCV) NS3/4A protease inhibitor with a K_i of 0.36 nM. Simeprevir inhibits HCV replication with an EC₅₀ of 7.8 nM. Simeprevir also potently suppresses SARS-CoV-2 replication and synergizes with Remdesivir. Simeprevir inhibits the main protease (M ^pro) and the RNA-dependent RNA polymerase (RdRp) of SARS-CoV-2, and also modulates host immune responses .

Grazoprevir 25+ Cited Publications MK-5172	HCV Protease HCV SARS-CoV	Infection
Grazoprevir (MK-5172) is a selective inhibitor of Hepatitis C virus NS3/4a protease with broad activity across genotypes and resistant variants, with K_is of 0.01 nM (gt1b), 0.01 nM (gt1a), 0.08 nM (gt2a), 0.15 nM (gt2b), 0.90 nM (gt3a), respectively . Grazoprevir inhibits SARS-CoV-2 3CL ^pro activity .

Palmatine chloride Maximum Cited Publications 9 Publications Verification	mAChR Indoleamine 2,3-Dioxygenase (IDO) Virus Protease Aurora Kinase Apoptosis Bacterial Parasite	Infection Neurological Disease Metabolic Disease Inflammation/Immunology Cancer
Palmatine chloride is an orally active and irreversible indoleamine 2,3-dioxygenase 1 (IDO-1) inhibitor with IC₅₀s of 3 μM and 157μM against HEK 293-hIDO-1 and rhIDO-1, respectively. Palmatine chloride can also inhibit West Nile virus (WNV) NS2B-NS3 protease in an uncompetitive manner with an IC₅₀ of 96 μM. Palmatine chloride shows anti-cancer, anti-oxidation, anti-inflammatory, neuroprotection, antibacterial, anti-viral activities .

Boceprevir 30+ Cited Publications EBP 520; SCH 503034	HCV Protease HCV SARS-CoV	Infection
Boceprevir (EBP 520) is a potent, highly selective, orally bioavailable HCV NS3 protease inhibitor with a K_i of 14 nM in both enzyme assay and an EC₉₀ of 350 nM in cell-based replicon assay . Boceprevir inhibits SARS-CoV-2 3CL ^pro activity .

Paritaprevir 10+ Cited Publications ABT-450; Veruprevir	HCV Protease HCV SARS-CoV	Infection
Paritaprevir (ABT-450) is a potent, orally active and antiviral non-structural protein 3/4A (NS3/4A) protease inhibitor with EC₅₀s of 1 and 0.21 nM against HCV 1a and 1b, respectively. Paritaprevir is also a SARS-CoV 3CL ^pro inhibitor with an IC₅₀ of 1.31 μM. Paritaprevir is metabolized primarily by cytochrome P450 (CYP) 3A. The plasma concentration and half-life of Paritaprevir can be enhanced by Ritonavir (a CYP450 inhibitor) .

Voxilaprevir GS-9857	HCV Protease	Infection Cancer
Voxilaprevir (GS-9857) is a noncovalent, reversible inhibitor of HCV NS3/4A protease inhibitor (PI) with pangenotypic antiviral activity . Voxilaprevir inhibits genotype 1b and 3a wild-type NS3 proteases with K_i values of 0.038 nM and 0.066 nM, respectively . Voxilaprevir is an orally active direct-acting antiviral agent (DAA) and can be used for HCV infection research .

Danoprevir 15+ Cited Publications ITMN-191; R7227; RO5190591; RG7227	HCV Protease HCV SARS-CoV	Infection
Danoprevir (ITMN-191) is an orally active **NS3/4A protease inhibitor for hepatitis C virus (HCV) with an IC₅₀** of 0.29 nM and is selective for NS3/4A over a panel of 53 proteases (IC₅₀ higher than 10 μM). Danoprevir (ITMN-191) inhibits HCV genotypes 1a, 1b, 4, 5, and 6 (IC₅₀s=0.2-0.4 nM) as well as 2b and 3a (IC₅₀s=1.6, 3.5 nM) . Danoprevir is also a SARS-CoV 3CL ^pro inhibitor with an IC₅₀ of 0.05 μM .

Pyridostatin TFA 30+ Cited Publications RR82 TFA	DNA/RNA Synthesis G-quadruplex Flavivirus Src Virus Protease	Cancer
Pyridostatin (RR82) TFA is a G-quadruplex (G4) inducer/stabilizer with a K_d of 490 nM. Pyridostatin TFA also acts as an inhibitor of Zika virus (ZIKV) *NS2B-NS3* protease, with an IC₅₀ of 11.0 μM. Pyridostatin TFA interacts with G-quadruplex structures, regulates the expression of SRC and SUB1, and induces replication- and transcription-dependent DNA damage, growth arrest, and genomic instability. Pyridostatin TFA exhibits antiproliferative and antiviral activities. Pyridostatin TFA can be used in studies related to breast cancer, cervical cancer, and Zika virus infection .

Palmatine	mAChR Indoleamine 2,3-Dioxygenase (IDO) Virus Protease Aurora Kinase Apoptosis Bacterial Parasite	Infection Neurological Disease Metabolic Disease Inflammation/Immunology Cancer
Palmatine is an orally active and irreversible indoleamine 2,3-dioxygenase 1 (IDO-1) inhibitor with IC₅₀s of 3 μM and 157μM against HEK 293-hIDO-1 and rhIDO-1, respectively. Palmatine can also inhibit West Nile virus (WNV) NS2B-NS3 protease in an uncompetitive manner with an IC₅₀ of 96 μM. Palmatine shows anti-cancer, anti-oxidation, anti-inflammatory, neuroprotection, antibacterial, anti-viral activities .

Pheophytin a	ERK Reactive Oxygen Species (ROS) COX PGE synthase STAT HCV HCV Protease	Infection Neurological Disease Cancer
Pheophytin a is a multi-target inhibitor, anticancer agent, antioxidant and antiviral agent. Pheophytin a directly binds to and inhibits *HCV-NS3/4A* protease (IC₅₀=0.89 μM) to block viral replication. Pheophytin a also scavenges free radicals, reduces ferric ions, and exhibits cytotoxic activity against breast cancer cells. Pheophytin a effectively inhibits LPS-induced production of nitric oxide, prostaglandin E2, NOS2 and COX-2, as well as various pro-inflammatory cytokines, by downregulating the transcription levels of inflammatory mediators and blocking the ERK1/2 and STAT-1 pathways. In a low nerve growth factor environment, Pheophytin a also enhances ERK1/2 phosphorylation and synergistically promotes neurite outgrowth through MAPK pathway. Pheophytin a can be used to investigate the pathogenic mechanisms of diseases including chronic hepatitis C, sepsis, breast cancer and Alzheimer's disease .

JNJ-40418677	γ-secretase Amyloid-β	Neurological Disease
JNJ-40418677 is an orally active modulator of γ-secretase, can cross the blood-brain barrier. JNJ-40418677 inhibits Aβ42 and NS2B-NS3 protease, with IC₅₀s of 200 nM and 3.9 μM, respectively. JNJ-40418677 displays good biological tolerance, can be use for Alzheimer’s disease research .

Grazoprevir potassium salt 25+ Cited Publications MK-5172 potassium salt	HCV HCV Protease SARS-CoV	Infection
Grazoprevir potassium salt (MK-5172 potassium salt) is a selective inhibitor of Hepatitis C virus NS3/4a protease with broad activity across genotypes and resistant variants, with K_is of 0.01 nM (gt1b), 0.01 nM (gt1a), 0.08 nM (gt2a), 0.15 nM (gt2b), 0.90 nM (gt3a), respectively . Grazoprevir potassium salt inhibits SARS-CoV-2 3CL ^pro activity .

Pyridostatin trihydrochloride 30+ Cited Publications RR82 trihydrochloride	DNA/RNA Synthesis G-quadruplex Flavivirus Src Virus Protease	Cancer
Pyridostatin (RR82) trihydrochloride is a G-quadruplex (G4) inducer/stabilizer with a K_d of 490 nM. Pyridostatin trihydrochloride also acts as an inhibitor of Zika virus (ZIKV) *NS2B-NS3* protease, with an IC₅₀ of 11.0 μM. Pyridostatin trihydrochloride interacts with G-quadruplex structures, regulates the expression of SRC and SUB1, and induces replication- and transcription-dependent DNA damage, growth arrest, and genomic instability. Pyridostatin trihydrochloride exhibits antiproliferative and antiviral activities. Pyridostatin trihydrochloride can be used in studies related to breast cancer, cervical cancer, and Zika virus infection .

Narlaprevir 5 Publications Verification SCH 900518	HCV HCV Protease SARS-CoV	Infection
Narlaprevir (SCH 900518) is a selective and orally bioavailable **NS3 protease inhibitor with a K_i value of 6 nM and an EC₉₀** value of 40 nM . Narlaprevir also inhibits the HCV nonstructural protein 3 serine protease . Narlaprevir is also a SARS-CoV 3CL ^pro inhibitor with an IC₅₀ of 2.3 μM .

Policresulen	Virus Protease Dengue Virus	Infection Inflammation/Immunology
Policresulen is a competitive inhibitor for DENV2 NS2B/NS3 protease with an IC₅₀ of 0.48 μg/mL. Policresulen can be used as a local hemostatic and antibacterial agent for research of cervical and vaginal inflammation, skin lesions, oral mucosa and gingival inflammation .

Ciluprevir BILN 2061; BILN 2061ZW	HCV HCV Protease DNA/RNA Synthesis	Infection
Ciluprevir (BILN 2061; BILN 2061ZW) is an orally active macrocyclic peptide inhibitor of hepatitis C virus (HCV)NS3 protease, with an IC₅₀ of 3 nM. Ciluprevir has Kᵢ values of 0.66 nM and 0.30 nM against genotypes 1b and 1a, respectively. Ciluprevir inhibits HCV RNA replication with an EC₅₀ of 1.2 nM, and its EC50 values against genotypes 1b and 1a are 3 nM and 4 nM, respectively. Ciluprevir shows no significant inhibition against human leukocyte elastase and hepatic cathepsin B. Ciluprevir can be used for genotype 1 HCV infection .

NS2B/NS3-IN-3	Virus Protease Flavivirus	Infection
NS2B/NS3-IN-3 (Compd 66) is an inhibitor of Flavivirus NS2B-NS3 protease .

BP13944	Dengue Virus	Infection
BP13944 is a potential small molecule inhibitor discovered by high-throughput screening. It can effectively inhibit the expression of dengue virus (DENV) replicons with an EC50 value of 1.03±0.09 μM. BP13944 can inhibit the replication or viral RNA synthesis of all four serotypes of DENV, but is ineffective against Japanese encephalitis virus. BP13944 may target the DENV NS3 protease, and the E66G amino acid substitution in the NS3 protease region will cause the virus to become resistant to BP13944. BP13944 has no obvious cytotoxicity. As there is currently no effective dengue vaccine and treatment, BP13944, as an effective small molecule inhibitor, may become a potential agent for the treatment of dengue in the future.

Primulin Primuline	Fluorescent Dye HCV Dengue Virus	Infection
Primulin is a versatile fluorescent dye and bioactive compound widely used in analytical, biological, botanical and virological studies. Primulin acts as a versatile stain that labels plant cell walls and differentiates live and dead spermatozoa via distinct fluorescence patterns. Primulin exhibits strong albumin‑binding capacity. Primulin acts as a retrograde axonal tracer in neurobiological investigations. Primulin and its derivatives inhibit HCV NS3, block dengue virus NS3-mediated ATP hydrolysis, and disrupt HCV replicase assembly .

Faldaprevir BI 201335	HCV Protease	Infection
Faldaprevir (BI 201335) is a potent, orally active and selective noncovalent inhibitor of **NS3/4A protease of HCV (hepatitis C virus) genotypes 1a and 1b, with K_i values of 2.6 and 2.0 nM, respectively. Faldaprevir inhibits HCV RNA replication, with EC₅₀** values of 6.5 and 3.1 nM, respectively. Faldaprevir has potent antiviral activity against chronic HCV infection .

Grazoprevir hydrate 25+ Cited Publications MK-5172 hydrate	HCV HCV Protease SARS-CoV	Infection
Grazoprevir hydrate (MK-5172 hydrate) is a selective inhibitor of Hepatitis C virus NS3/4a protease with broad activity across genotypes and resistant variants, with K_is of 0.01 nM (gt1b), 0.01 nM (gt1a), 0.08 nM (gt2a), 0.15 nM (gt2b), 0.90 nM (gt3a), respectively . Grazoprevir hydrate inhibits SARS-CoV-2 3CL ^pro activity .

Palmatine hydroxide Maximum Cited Publications 9 Publications Verification	mAChR Indoleamine 2,3-Dioxygenase (IDO) Virus Protease Aurora Kinase Apoptosis Bacterial Parasite	Infection Neurological Disease Metabolic Disease Inflammation/Immunology Cancer
Palmatine hydroxide is an orally active and irreversible indoleamine 2,3-dioxygenase 1 (IDO-1) inhibitor with IC₅₀s of 3 μM and 157μM against HEK 293-hIDO-1 and rhIDO-1, respectively. Palmatine hydroxide can also inhibit West Nile virus (WNV) NS2B-NS3 protease in an uncompetitive manner with an IC₅₀ of 96 μM. Palmatine hydroxide shows anti-cancer, anti-oxidation, anti-inflammatory, neuroprotection, antibacterial, anti-viral activities .

Rhodiolin 1 Publications Verification	Glucose-6-Phosphate Isomerase (GPI) Casein Kinase Virus Protease Flavivirus PI3K Akt mTOR Apoptosis	Infection Cancer
Rhodiolin, a flavonoid, is an orally active glucose 6-phosphate isomerase (GPI) inhibitor. Rhodiolin inhibits papillary thyroid cancer (PTC) by targeting glycolysis enzyme glucose 6-phosphate isomerase GPI and suppressing PI3K/AKT/mTOR phosphorylation and induce apoptosis. Rhodiolin as a *NS2B-NS3* protease inhibitor can disrupt dengue viral replication. Rhodiolin is also a potential candidate for developing anticancer strategies inhibiting CK1ε kinase. Rhodiolin can be used for the study of anti-tumor and anti-viral .

Vaniprevir MK-7009	HCV HCV Protease	Infection
Vaniprevir (MK-7009) is a non-covalent competitive inhibitor of the hepatitis C virus (HCV) **NS3/4A protease**.

ITMN 4077	HCV Protease	Infection
ITMN 4077 is a macrocyclic inhibitor against Hepatitis C Virus (HCV) **NS3 protease (EC₅₀**: 2131 nM) .

ZINC03129319	Flavivirus Dengue Virus Virus Protease	Infection
ZINC03129319 is a dengue virus (DENV) NS2B-NS3 protease inhibitor extracted from patent US20150141521A1, has inhibition constants (K_i1) of 92 μM and K_i3 of 20 μM.

DV-B-120	Dengue Virus Virus Protease	Infection
DV-B-120 is a competitive inhibitor for dengue virus (DENV) with IC₅₀s of 5.35, 7.39, 10.49 and 8.58 μM, for DENV-1, DENV-2, DENV-3 and DENV-4, respectively, by inhibiting NS2B-NS3 protease. DV-B-120 exhibits antiviral activity through inhibition of DENV replication .

BI 653048	Glucocorticoid Receptor Cytochrome P450 HCV Protease	Infection Inflammation/Immunology
BI 653048, a chemical probe, is a selective and orally active nonsteroidal glucocorticoid (GC) agonist with an IC₅₀ value of 55 nM . BI 653048 inhibits CP1A2, CYP2D6, CYP2C9, CYP2C19 and CYP3A4 isoforms’ activity and reduces affinity for the hERG ion channel (IC₅₀>30 μM) . BI 653048 (Compound 103) is also a HCV NS3 protease inhibitor that can reduce viral loads infected with the hepatitis C virus .

NS2B/NS3-IN-3 hydrochloride	Virus Protease Flavivirus	Infection
NS2B/NS3-IN-3 hydrochlorideis an inhibitor of Flavivirus NS2B-NS3 protease .

NS2B/NS3-IN-8	Virus Protease	Infection
NS2B/NS3-IN-8 (compound SM7) is a Zika virus NS2B-NS3 protease inhibitor .

*Hepatitis Virus C NS3* Protease Inhibitor 2**	HCV Protease HCV	Infection
Hepatitis Virus C NS3 Protease Inhibitor 2 is a product-based peptide inhibitor of hepatitis C virus (HCV) NS3 protease, with a K_i of 41 nM .

HCV NS4A Protein (18-40) (JT strain)	HCV	Infection
HCV NS4A Protein (18-40) (JT strain) is an HCV NS4A peptide, which comprised residues 18 to 40 of the NS4A protein and is known to increase the catalytic efficiency of NS3 protease .

Ac-EEVVAC-pNA	HCV Protease	Infection
Ac-EEVVAC-pNA is a chromogenic substrate for a continuous spectrophotometric assay of HCV NS3 protease. The sequence EEVVAC is derived from the 5A-5B cleavage junction of the HCV polyprotein .

6-Chloroindole-2-carboxylic acid	HCV HCV Protease	Infection
6-Chloroindole-2-carboxylic acid (Compound 10g) is a derivative of 2-carboxyindole. 6-Chloroindole-2-carboxylic acid can bind to the hepatitis C virus NS3 protease/NS4A cofactor complex, and its K_D value for the *ns4a-ns3p* protein is 0.4 mM.

HZ-1157	Flavivirus Dengue Virus HCV Protease	Infection Inflammation/Immunology
HZ-1157 inhibits HCV NS3/4A protease with an IC₅₀ of 1.0 μmol/L. HZ-1157 (4a) has a high dengue virus inhibitory activity (EC₅₀ = 0.15 μM) and is a relatively nontoxic (CC₅₀ > 10 μM) dengue antiviral agent .

BI 653048 phosphate	Glucocorticoid Receptor Cytochrome P450 HCV Protease	Infection Inflammation/Immunology
BI 653048 phosphate is a selective and orally active nonsteroidal glucocorticoid (GC) agonist with an IC₅₀ value of 55 nM . BI 653048 phosphate inhibits CP1A2, CYP2D6, CYP2C9, CYP2C19 and CYP3A4 isoforms’ activity and reduces affinity for the hERG ion channel (IC₅₀>30 μM) . BI 653048 phosphate is extracted from patent WO2005028501A1 (Compound 103), is also a HCV NS3 protease inhibitor that can reduce viral loads infected with the hepatitis C virus .

NS2B/NS3-IN-7	Virus Protease Flavivirus	Infection
NS2B/NS3-IN-7 (compound 26) is a highly potent Zika virus NS2B-NS3 protease inhibitor with a K_i value of 2.33 nM. NS2B/NS3-IN-7 can reduce amounts of ZIKV-infected cells .

NS2B/NS3-IN-9	Virus Protease Flavivirus Dengue Virus	Infection
NS2B/NS3-IN-9 (Compound 73) is a broad-spectrum, non-competitive anti-Orthoflavivirus lipopeptide inhibitor targeting the *NS2B-NS3* protease. NS2B/NS3-IN-9 exhibits IC₅₀ values for Dengue virus DENV2 NS2B-NS3, West Nile virus WNV NS2B-NS3, and Zika virus ZIKV NS2B-NS3 of 2.4, 7.2, and 1.9 μM, respectively. NS2B/NS3-IN-9 also exhibits antiviral activity at the cellular level against DENV2, WNV, and ZIKV, with EC₅₀ values of 4.1, 4.9, and 5.0 μM, respectively. NS2B/NS3-IN-9 has no significant toxicity to cells. NS2B/NS3-IN-9 can be used for the study of anti-Orthoflavivirus .

MK-1220	HBV HCV Protease	Infection
MK-1220 is a covalently reversible inhibitor of the hepatitis C virus (HCV) NS3/4A protease (NS3/4A *protease) with a K_i* of 0.02 nM. MK-1220 in cell models simulating viral replication exhibits EC₅₀s of 4 (with 10% fetal bovine serum) and 11 nM (50% normal human serum). MK-1220 can be used for the study of chronic hepatitis C virus infection .

BI-1230	HCV Protease	Infection
BI-1230, a chemical probe, is potent and digit nanomolar inhibitor of HCV NS3 protease and of viral replication. BI-1230 is also highly selective against other serine/cysteine proteases. BI-1230 shows good Pharmacokinetic(PK) activity .

4-Hydroxy-2-methylbenzenesulfonic acid ammonium	Virus Protease	Infection
4-Hydroxy-2-methylbenzenesulfonic acid ammonium is an impurity of Policresulen. Policresulen is a potent NS2B/NS3 protease inhibitor with an IC₅₀ of 0.48 μg/mL. Policresulen effectively inhibits the replication of DENV2 virus in BHK-21 cells with an IC₅₀ of 4.99 μg/mL. Policresulen acted as a competitive inhibitor of the protease, and slightly affected the protease stability .

BMS 605339	HCV Protease	Infection
BMS 605339 is a linear tetra-peptide α-ketoamide inhibitor of HCV NS3 protease. BMS 605339 can be used in research related to hepatitis C (HCV infection) .

Cat. No.	Product Name	Type

HY-P4039

Ac-EEVVAC-pNA	Fluorescent Dyes
Ac-EEVVAC-pNA is a chromogenic substrate for a continuous spectrophotometric assay of HCV NS3 protease. The sequence EEVVAC is derived from the 5A-5B cleavage junction of the HCV polyprotein .

Cat. No.	Product Name	Type

HY-W540972

Primulin

Primuline

Biochemical Assay Reagents

Primulin is a versatile fluorescent dye and bioactive compound widely used in analytical, biological, botanical and virological studies. Primulin acts as a versatile stain that labels plant cell walls and differentiates live and dead spermatozoa via distinct fluorescence patterns. Primulin exhibits strong albumin‑binding capacity. Primulin acts as a retrograde axonal tracer in neurobiological investigations. Primulin and its derivatives inhibit HCV NS3, block dengue virus NS3-mediated ATP hydrolysis, and disrupt HCV replicase assembly .

Cat. No.	Product Name	Target	Research Area

HY-P2502

*Hepatitis Virus C NS3* Protease Inhibitor 2**	HCV Protease HCV	Infection
Hepatitis Virus C NS3 Protease Inhibitor 2 is a product-based peptide inhibitor of hepatitis C virus (HCV) NS3 protease, with a K_i of 41 nM .

HY-P4209

Boc-GRR-AMC	Peptides	Others
Boc-GRR-AMC is a tri-peptide Substrate. Boc-GRR-AMC can be used for a fluorogenic West Nile virus (WNV) substrate, profiling the substrate specificity for the NS2B-NS3 proteases or determining the pH optimum of LdMC activity .

HY-P4035

HCV NS4A Protein (18-40) (JT strain)	HCV	Infection
HCV NS4A Protein (18-40) (JT strain) is an HCV NS4A peptide, which comprised residues 18 to 40 of the NS4A protein and is known to increase the catalytic efficiency of NS3 protease .

HY-P4039

Ac-EEVVAC-pNA	HCV Protease	Infection
Ac-EEVVAC-pNA is a chromogenic substrate for a continuous spectrophotometric assay of HCV NS3 protease. The sequence EEVVAC is derived from the 5A-5B cleavage junction of the HCV polyprotein .

HY-P5510

Ac-Asp-Glu-Asp(EDANS)-Glu-Glu-Abu-ψ-(COO)Ala-Ser-Lys(DABCYL)-NH2 HCV NS3 protease substrate	Peptides	Others
Ac-Asp-Glu-Asp(EDANS)-Glu-Glu-Abu-ψ-(COO)Ala-Ser-Lys(DABCYL)-NH2 (HCV NS3 protease substrate) is a biological active peptide. (This peptide is a HCV protease substrate incorporating an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM.

HY-P10657

Ac-DEMEEC-OH	HCV HCV Protease	Infection
Ac-DEMEEC-OH is a HCV NS3 protease competitive inhibitor (K_i: 0.6 µM) .

HY-P10608

KK4A peptide	HCV	Infection
KK4A peptide is a small peptide derived from nonstructural protein 4A (NS4A) of hepatitis C virus (HCV) that significantly increases the catalytic efficiency of NS3 protease. The KK4A peptide may be used to gain insights into the function and mechanism of the HCV NS3 protease, thereby advancing research into HCV infection .

HY-P5312

Abz-Gly-Leu-Lys-Arg-Gly-Gly-3-(NO2)Tyr	Virus Protease	Infection
Abz-Nle-Lys-Arg-Arg-Ser-3-(NO2)Tyr is a WNV NS2B-NS3 protease substrate. Abz-Nle-Lys-Arg-Arg-Ser-3-(NO2)Tyr is used for WNV NS2B-NS3 protease substrate peptide selective inhibitor development .

HY-P2435

Bz-Nle-Lys-Arg-Arg-AMC	Peptides	Others
Bz-Nle-Lys-Arg-Arg-AMC can be used for protease activity assay. In 0.1mL Tris-HCl buffer (50 mM, pH 7.8), Bz-Nle-Lys-Arg-Arg-AMC was hydrolyzed, and the release rate of AMC was observed .

HY-P4209A

Boc-GRR-AMC TFA	Virus Protease	Others
Boc-GRR-AMC (TFA) is a tri-peptide Substrate. Boc-GRR-AMC can be used for a fluorogenic West Nile virus (WNV) substrate, profiling the substrate specificity for the NS2B-NS3 proteases or determining the pH optimum of LdMC activity .

HY-P4038

Hepatitis C Virus S5A/5B	HCV Protease	Infection
Hepatitis C Virus S5A/5B is a synthetic peptide substrate. Hepatitis C Virus S5A/5B mimics the NS5A/5B junction of the nonstructural protein (NS), served as the substrate for the study of HCV NS3 protease activity .

HY-P5312A

Abz-Gly-Leu-Lys-Arg-Gly-Gly-3-(NO2)Tyr acetate	Virus Protease	Infection
Abz-Gly-Leu-Lys-Arg-Gly-Gly-3-(NO2)Tyr acetate is the acetate salt form of Abz-Gly-Leu-Lys-Arg-Gly-Gly-3-(NO2)Tyr. Abz-Gly-Leu-Lys-Arg-Gly-Gly-3-(NO2)Tyr acetate is the substrate for WNV NS2B NS3 protease, which is utilized for the development of WNV protease substrate peptide selective inhibitor .

HY-P11420

*2A/2B Dengue protease* substrate**	Dengue Virus	Infection
2A/2B Dengue protease substrate (Ac-RTSKKR-pNA) is a peptide substrate for Dengue NS2B-NS3 protease. 2A/2B Dengue protease substrate is used for Dengue NS2B-NS3 protease substrate peptide selective inhibitor development .

HY-P5642A

Retrocyclin-101 TFA RC-101 TFA	HIV SARS-CoV Influenza Virus Flavivirus HSV Bacterial Fungal Toll-like Receptor (TLR) Ser/Thr Protease	Infection Inflammation/Immunology
Retrocyclin-101 (RC-101) TFA is an artificially synthesized, cyclic-structured θ-defensin, a broad-spectrum agent with antimicrobial (covering viruses, bacteria, and fungi) activity and anti-inflammatory activity. Retrocyclin-101 TFA can inhibit the serine protease activity of ZIKV NS2B-NS3, with an IC₅₀ of 7.20 μM. Retrocyclin-101 TFA has significant inhibitory activity against HIV, SARS-CoV-2, flaviviruses, influenza viruses, HSV-1/2, Staphylococcus aureus, etc. Retrocyclin-101 TFA inhibits the signal transduction mediated by TLR4 and TLR2, reducing the expression of pro-inflammatory cytokines .

Cat. No.	Compare	Product Name	Species	Source	Image

HY-P74898

	NS3 protease Protein, HCV (GST) Hepatitis C virus (HCV-1a) NS3 protease / helicase immunodominant region Protein ( GST)	Virus	E. coli
	NS3 protease Protein is a zinc-dependent serine protease.NS3 needs to bind to the viral protein NS4A to enable additional conformation changes that enhance activity and appropriate intracellular localization.NS3 protein induces Caspase-8-mediated apoptosis independently of its protease or helicase activity.NS3 protease Protein, HCV (GST) is the recombinant Virus-derived NS3 protease protein, expressed by E.coli , with N-GST labeled tag.

Compare Products


Products	In-stock - + Add to Cart
Cat. No.
Species
Source
Tag
Accession
Gene ID
Molecular Weight
Purity
Endotoxin Level
Biological Activity
Appearance
Formulation
Storage & Stability
Shipping
Free Sample	Yes No
Size	* This product has been "discontinued". Optimized version of product available: / In-stock - + Add to Cart Get quote

Cat. No.	Product Name	Chemical Structure

HY-10235S

Telaprevir-d4
Telaprevir-d₄ is the deuterium labeled Telaprevir. Telaprevir (VX-950) is a highly selective, reversible, and potent peptidomimetic inhibitor of the HCV NS3-4A protease, the steady-state inhibitory constant (Ki) of Telaprevir is 7 nM against a genotype 1 (H strain) NS3 protease domain plus a NS4A cofactor peptide . Telaprevir inhibits SARS-CoV-2 3CLpro activity .

HY-10241S

Simeprevir-13C,d3

Simeprevir- ¹³C,d₃ is the ¹³C- and deuterium labeled Simeprevir. Simeprevir is an oral, potent and highly specific hepatitis C virus (HCV) NS3/4A protease inhibitor with a K_i of 0.36 nM. Simeprevir inhibits HCV replication with an EC₅₀ of 7.8 nM. Simeprevir also potently suppresses SARS-CoV-2 replication and synergizes with Remdesivir. Simeprevir inhibits the main protease (Mpro) and the RNA-dependent RNA polymerase (RdRp) of SARS-CoV-2, and also modulates host immune responses .

HY-131905S

BMS-986144
BMS-986144 is a third-generation, pan-genotype (GT) **NS3/4A protease inhibitor. BMS-986144 inhibits HCV replicon with EC₅₀**s of 2.3, 0.7, 1.0, 12, 8.0, and 5.8 nM for GT-1a, GT-1b, GT-2a, GT-3a, 1a R155X, and 1b D168V, respectively. BMS-986144 has the potential for the research of HCV infection .

HY-10237S

Boceprevir-d9
Boceprevir-d₉ is the deuterium labeled Boceprevir. Boceprevir (EBP 520) is a potent, highly selective, orally bioavailable HCV NS3 protease inhibitor with a K_i of 14 nM in both enzyme assay and an EC₉₀ of 350 nM in cell-based replicon assay . Boceprevir inhibits SARS-CoV-2 3CLpro activity .

Inquiry Online

Your information is safe with us. * Required Fields.

MCE Japan Authorized Agent ナミキ商事株式会社コスモ・バイオ株式会社重松貿易株式会社
Salutation			Country or Region *
Applicant Name *			Organization Name *
Department *
Email Address *			Product Name *
Cat. No.			Requested quantity *
Phone Number *
Remarks

Inquiry Online

Inquiry Information

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

NS3 protease

Inhibitors & Agonists

Fluorescent Dyes

Biochemical Assay Reagents

Peptides

NaturalProducts

Recombinant Proteins

Isotope-Labeled Compounds

Natural
Products