2. Apoptosis Epigenetics Cell Cycle/DNA Damage
  3. Apoptosis PARP
  4. Sino-C

Sino-C is a Sinomenine (HY-15122) derivative with anticancer activity. Sino-C broadly disrupts cholesterol homeostasis by upregulating key genes such as SREBF2 and HMGCS1, leading to intracellular cholesterol accumulation and lipid droplet formation. Sino-C-induced metabolic dysregulation further triggers lipid peroxidation and endoplasmic reticulum (ER) stress, initiating a unique form of hybrid cell death including apoptotic (cleaved PARP) and necrotic-like features. Sino-C thus serves as a useful compound for research in colorectal cancer, lung cancer and breast cancer.

For research use only. We do not sell to patients.

Sino-C

Sino-C Chemical Structure

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Sino-C Related Antibodies

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Description

Sino-C is a Sinomenine (HY-15122) derivative with anticancer activity. Sino-C broadly disrupts cholesterol homeostasis by upregulating key genes such as SREBF2 and HMGCS1, leading to intracellular cholesterol accumulation and lipid droplet formation. Sino-C-induced metabolic dysregulation further triggers lipid peroxidation and endoplasmic reticulum (ER) stress, initiating a unique form of hybrid cell death including apoptotic (cleaved PARP) and necrotic-like features. Sino-C thus serves as a useful compound for research in colorectal cancer, lung cancer and breast cancer[1].

In Vitro

Sino-C (0-20 μM, 48 h) exhibits broad-spectrum anticancer activity in CRC (HCT116, RKO, and MC38), lung cancer (A549 and NCI-H1299), and breast cancer (MDA-MB-231 and 4T1) cells[1].
Sino-C (0-20 μM, 24 h) exhibits significant anti-CRC effect in HCT116 and RKO cells[1].
Sino-C (0-20 μM, 24 h) induces HCT116 and RKO cell death, characterized by PARP cleavage without caspase-3 activation, causes severe morphological alterations (widened perinuclear spaces, extensive ER dilation with ribosome detachment, mitochondrial swelling) along with significant LDH leakage and membrane injury, suggesting a non-apoptotic mechanism[1].
Sino-C (24 h) elicits a unique form of cell death: the morphological features do not match those of classical apoptosis, paraptosis, pyroptosis, or ferroptosis, but instead represent a hybrid mode combining both apoptotic and necrotic-like characteristics in HCT116 cells[1].
Sino-C (0-20 μM, 24 h) upregulates cholesterol homeostasis-related genes, elevates intracellular cholesterol levels, and induces lipid droplet accumulation in HCT116 cells[1].
Sino-C (0-20 μM, 24 h) induces cholesterol dysregulation, which leads to lipid peroxidation and subsequently triggers endoplasmic reticulum (ER) stress, ultimately contributing to CRC cell death[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Sino-C Related Antibodies

Cell Viability Assay[1]

Cell Line: HCT116, RKO, MC38, A549, NCI-H1299, MDA-MB-231, 4T1
Concentration: 1.25, 2.5, 5, 10 and 20 μM
Incubation Time: 48 h
Result: Significantly reduced cell viability in all tested cell lines in a dose-dependent manner, with IC50 values predominantly below 10 μM.
Showed greater potency against CRC cell lines, with IC50 values of 5.15 μM (HCT116), 9.72 μM (RKO), and 4.58 μM (MC38).

Cell Proliferation Assay[1]

Cell Line: HCT116 and RKO cells
Concentration: 0, 5, 10 and 20 μM
Incubation Time: 24 h
Result: Remarkably inhibited the colony formation of HCT116 and RKO cells.

Apoptosis Analysis[1]

Cell Line: HCT116 and RKO cells
Concentration: 0, 5, 10 and 20 μM
Incubation Time: 24 h
Result: Revealed a dose-dependent increase in apoptosis: from 5.81% (control) to 26.80% (20 μM) in HCT116 cells from 5.39% to 22.34% in RKO cells.
Had minimal Annexin V+ staining in both cell lines, indicating a limited externalization of phosphatidylserine on the plasma membrane.
Increased in PI+ stained cells upon Sino-C treatment, suggesting that apoptosis may not be the only cell death mode.

RT-PCR[1]

Cell Line: HCT116 cells
Concentration: 10 μM
Incubation Time: 24 h
Result: Upregulated key genes involved in cholesterol homeostasis, including the master transcriptional Regulator of cholesterol biosynthesis, SREBF2, as well as rate-limiting enzymes and a key Receptor in the pathway, such as HMGCS1, SQLE, and LDLR.

Immunofluorescence[1]

Cell Line: HCT116 cells
Concentration: 5, 10 and 20 μM
Incubation Time: 24 h
Result: Markedly elevated intracellular cholesterol levels.
In Vivo

Sino-C (30 and 50 mg/kg, i.t., every other day from day 14 to day 28) exhibits significant anti-CRC effect in HCT116 cells induce-BALB/c nude mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: HCT116 cells (1 × 106, s.c.) induce- BALB/c nude mice[1]
Dosage: 30 and 50 mg/kg
Administration: i.t., every other day from day 14 to day 22 (30 mg/kg) then i.t., every other day from day 24 to day 28 (50 mg/kg)
Result: Significantly reduced tumor size and average tumor weight by approximately 43.24%.
Suppressed tumor growth over the two-week study period.
Caused no significant changes in mouse body weight, indicating minimal systemic toxicity.
Molecular Weight

506.44

Formula

C25H25Cl2NO4S
SMILES

O=C1C[C@@]2(CCN3C)C4=C(O)C(O)=CC=C4[C@@H](SCC5=CC=C(Cl)C=C5Cl)[C@@]3([H])C2C=C1OC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Sino-C Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sino-CApoptosisPARPpoly ADP ribose polymeraseintracellular cholesterolmetabolic dysregulationcolorectal cancerlung cancerbreast cancer,SREBF2,HMGCS1apoptoticInhibitorinhibitorinhibit

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