Sulfo-Cyanine5.5 carboxylic acid

Guide (The following is our recommended protocol. This protocol is for guidance only and should be modified according to your specific needs).

1. Stock Solution Preparation
1.1 Solvent: PBS.
1.2 Concentration recommendation: 2 mg/mL.

2. Working Solution Preparation
2.1 Diluent: Aqueous solution.
2.2 Working concentration: 5 μM.
2.3 Note: Adjust working solution concentration as needed; prepare fresh before use.

3. Staining Procedure
3.1 Sample type: Multicompartmental synthetic microstructures[1]
3.2 Incubation conditions: Incubate with 5 μM sulfo-Cyanine5.5 carboxylic acid working solution.
3.3 Washing steps: No washing steps specified.

3.1 Sample type: In vivo murine cerebral vascular tissue[2]
3.2 Incubation conditions: Administer 50 μL of 2 mg/mL sulfo-Cyanine5.5 carboxylic acid stock solution via tail vein injection; record fluorescence imaging for 20 s immediately after injection.
3.3 Washing steps: No washing steps specified.

5. Detection & Analysis
5.1 Instrument type: Fluorescence microscope.
5.2 Ex/Em wavelengths: Peak absorption at 673 nm; red fluorescence emission (corresponding to sulfo-Cyanine5.5 carboxylic acid).
5.3 Result analysis:
5.3.1 For multicompartmental synthetic microstructures[1]:
5.3.1.1 Fluorescence intensity of sulfo-Cyanine5.5 carboxylic acid is much higher in the water phase dark spots than in the polymer-enriched phase.
5.3.1.2 Fluorescence localizes to the water phase subcompartments of the multicompartmental microstructures.
5.3.1.3 Red fluorescence is emitted from sulfo-Cyanine5.5 carboxylic acid.
5.3.2 For in vivo murine cerebral vascular tissue[2]:
5.3.2.1 Fluorescence intensity changes can be used to map cerebral perfusion dynamics, with reduced intensity indicating perfusion deficits in ischemic regions.
5.3.2.2 Fluorescence localizes to cerebral vascular networks, enabling differentiation between arterial, venous, and skull vessels via post-acquisition analysis.
5.3.2.3 Multicolor fluorescence mapping can be used to visualize parameters such as blood flow index, mean transient time, and time to peak.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.