TRPC5-IN-4
TRPC5-IN-4 is potent and safe TRPC inhibitor with IC50 value of 14.07 nM and 65 nM for TRPC5 and TRPC4, respectively. TRPC5-IN-4 shows no damage on the cellular component of liver and kidney. TRPC5-IN-4 can be used for the research of chronic kidney disease (CKD).
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- CAS No.: 2762315-39-7
- Formule: C18H11ClF4N4O3
- Masse moléculaire:442.75
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
IC50 & Target
[1]|
TRPC5 14.07 nM (IC50) |
TRPC4 65 nM (IC50) |
In Vitro
TRPC5-IN-4 (compound 16 g) (0.003-3 μM; 0-1000 seconds) significantly changes intracellular Ca2+ concentration in TRPC4- and TRPC5-HEK293 cells in a dose-dependent manner, and shows very weak inhibitory activity on the TRPC3 channel, no inhibitory effect on TRPC6 and TRPC7 channels[1].
TRPC5-IN-4 (1 and 10 μM; 24 hours) does not reduce the liver and kidney cells viability[1].
TRPC5-IN-4 (0.1, 0.3, 1, 3 μM; 30 minutes) concentration-dependently reduces the PS-induced podocyte rearrangement in MPC5 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:Primary cultured hepatocytes and MPC5 cells[1]
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Concentration:1 and 10 μM
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Incubation Time:24 hours
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Result:Did not reduce the cell viability, suggesting that TRPC5-IN-4 was unlikely to damage the cellular component of liver and kidney.
Chemical Information
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CAS No. 2762315-39-7
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Masse moléculaire 442.75
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Formule C18H11ClF4N4O3
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SMILES
O=C1NC(C(N2CCC3=C(N=CN=C3OC4=C(C=C(C=C4)F)C(F)(F)F)C2)=C1Cl)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
TRPC5-IN-4 Related Classifications
HY-144429 Related Classifications
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