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Cat. No.: HY-162625

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Tubulin inhibitor 47

Tubulin inhibitor 47 (compound C11) is a potent tubulin inhibitor. Tubulin inhibitor 47 induces apoptosis and cell cycle arrest at the M phase. Tubulin inhibitor 47 shows anti-tumor activity.

For research use only. We do not sell to patients.

CAS No.: 3048072-30-3
Formula: C₂₀H₁₆N₂O₂
Molecular Weight:316.35

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (276 KB)

Tubulin inhibitor 47 Chemical Structure

CAS No.: 3048072-30-3

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Tubulin inhibitor 47 (compound C11) is a potent tubulin inhibitor. Tubulin inhibitor 47 induces apoptosis and cell cycle arrest at the M phase. Tubulin inhibitor 47 shows anti-tumor activity^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	0.026 μM Compound: C11	Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay	[PMID: 38901104]
HUVEC	IC₅₀	>0.71 μM Compound: C11	Cytotoxicity against HUVEC cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Cytotoxicity against HUVEC cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 38901104]
KYSE-270	IC₅₀	41.763 nM Compound: C11	Antiproliferative activity against human KYSE-270 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Antiproliferative activity against human KYSE-270 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 38901104]
KYSE-30	IC₅₀	0.013 μM Compound: C11	Antiproliferative activity against human KYSE-30 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay Antiproliferative activity against human KYSE-30 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay	[PMID: 38901104]
KYSE-30	IC₅₀	13.148 nM Compound: C11	Antiproliferative activity against human KYSE-30 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay Antiproliferative activity against human KYSE-30 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay	[PMID: 38901104]
KYSE-450	IC₅₀	19.159 nM Compound: C11	Antiproliferative activity against human KYSE-450 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Antiproliferative activity against human KYSE-450 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 38901104]
MGC-803	IC₅₀	0.014 μM Compound: C11	Antiproliferative activity against human MGC-803 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay Antiproliferative activity against human MGC-803 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay	[PMID: 38901104]

In Vitro

Tubulin inhibitor 47 (compound C11) targets the colchicine site of tubulin to inhibit its polymerization^[1].
Tubulin inhibitor 47 (0, 2, 4, 6, 8, 10 nM) induces cell cycle arrest at the M phase^[1].
Tubulin inhibitor 47 (0, 5, 10, 20 nM) increases the expression of p-Histone H3 in KYSE30 cells in a dose-dependent manner^[1].
Tubulin inhibitor 47 (0, 5, 10, 20 nM; 48 h) induces apoptosis in a dose-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

MGC-803, KYSE-30, HCT-116 cells
Concentration:

0-10 µM
Incubation Time:

48 h
Result:

Showed antiproliferative activities with IC₅₀s of 0.014, 0.013, 0.026 µM for MGC-803, KYSE-30, HCT-116 cells, respectively.

Apoptosis Analysis^[1]

Cell Line:

KYSE30, KYSE70 cells
Concentration:

5, 10, 20 nM
Incubation Time:

48 h
Result:

Induced apoptosis and decreased the expression of cleaved-PARP, cleaved-caspase 9/7/3 protein.

Cell Cycle Analysis^[1]

Cell Line:

KYSE30, KYSE70 cells
Concentration:

5, 10, 20 nM
Incubation Time:
Result:

Induced cell cycle arrest at M phase in a dose-dependent manner.

In Vivo

Tubulin inhibitor 47 (6, 12 mg/kg; i.p.; daily for 15 days) shows anti-tumor activity in a dose-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

6-8 weeks, female BALA/c-nu nude mice (KYSE30 cells)^[1]
Dosage:

6, 12 mg/kg
Administration:

I.p.; daily for 15 days
Result:

Decreased tumor growth in a dose-dependent manner and showed no significant changes in animal weight during treatment.

Chemical Information

CAS No. 3048072-30-3
Molecular Weight 316.35
Formula C₂₀H₁₆N₂O₂
SMILES

CC(C=C1C2=C(C3=CC=C(OC)C=C3)C=NO2)=NC4=C1C=CC=C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Jia M, et al. Synthesis and biological evaluation of 4-phenyl-5-quinolinyl substituted isoxazole analogues as potent cytotoxic and tubulin polymerization inhibitors against ESCC. Eur J Med Chem. 2024 Jun 18;275:116611. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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