2. Cell Cycle/DNA Damage Cytoskeleton Apoptosis
  3. Microtubule/Tubulin Apoptosis
  4. Tubulin polymerization-IN-13

Tubulin polymerization-IN-13 (Compound 4f) is a tubulin polymerization inhibitor (IC50=0.37 μM). Tubulin polymerization-IN-13 shows anti-proliferative activity against cancer cells, induces apoptosis and potential antivascular activity.

For research use only. We do not sell to patients.

Tubulin polymerization-IN-13 Chemical Structure

Tubulin polymerization-IN-13 Chemical Structure

CAS No. : 2426665-56-5

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  
Synthetic products have potential research and development risk.

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Tubulin polymerization-IN-13 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Tubulin polymerization-IN-13 (Compound 4f) is a tubulin polymerization inhibitor (IC50=0.37 μM). Tubulin polymerization-IN-13 shows anti-proliferative activity against cancer cells, induces apoptosis and potential antivascular activity[1].

IC50 & Target

IC50: 0.37 μM (tubulin polymerization)[1]
In Vitro

Tubulin polymerization-IN-13 (0.005-2.8 nM) treatment inhibits tumor cell proliferation[1].
Tubulin polymerization-IN-13 (8.7-10 μM) is non-toxic in non-tumor cells[1].
Tubulin polymerization-IN-13 (1-50 nM; 24 h) treatment induces cell cycle arrest in G2/M[1].
Tubulin polymerization-IN-13 (10 nM; 24 and 48 h) treatment induces cell apoptosis[1].
Tubulin polymerization-IN-13 (10-100 nM; 24 h) treatment induces alteration of the microtubule network[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tubulin polymerization-IN-13 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: HeLa, HT-29, Daoy, HL-60, SEM, and Jurkat cells
Concentration: 0.005-2.8 nM
Incubation Time:
Result: Showed IC50s of 2.8 nM, 2.1 nM, 0.005 nM, 2.7 nM, 0.31 nM, and 0.28 nM for HeLa, HT-29, Daoy, HL-60, SEM, and Jurkat cells, respectively.

Cell Cytotoxicity Assay[1]

Cell Line: Peripheral blood lymphocytes (PBL)
Concentration: 8.7-10 μM
Incubation Time:
Result: Showed a GI50 of 8.7 μM in quiescent lymphocytes.

Cell Cycle Analysis[1]

Cell Line: HeLa cells
Concentration: 1, 5, 10, and 50 nM
Incubation Time: 24 hours
Result: Induced a G2/M arrest at 10 nM, increased G2/M cells accompanied by a strong reduction of cells in the G1 phase.

Apoptosis Analysis[1]

Cell Line: HeLa cells
Concentration: 10 nM
Incubation Time: 24 and 48 hours
Result: Induced caspase-9 activation, PARP cleavage, Bcl-2 phosphorylation and Mcl-1 downregulation.

Immunofluorescence[1]

Cell Line: HeLa cells
Concentration: 10, 50, and 100 nM
Incubation Time: 24 hours
Result: Showed the disorganization of microtubules at 10 nM, and much more evident at 50 and 100 nM.
In Vivo

Tubulin polymerization-IN-13 (intraperitoneal injection; 15 or 5 mg/kg; once every other day; 4 times) reduces tumor growth in a dose-dependent manner in an orthotopic murine tumor model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Seven-week-old C57BL/6 female mice orthotopically injected into the mammary fat pad with E0771 mammary carcinoma cells[1]
Dosage: 15 or 5 mg/kg
Administration: Intraperitoneal injection; 15 or 5 mg/kg; once every other day; 4 times
Result: Reduced tumor mass by 45.7% and 16.9% at 15 and 5 mg/kg, respectively.
Showed no sign of toxicity and no decreasement in animal body weight.
Molecular Weight

371.38

Formula

C20H21NO6
CAS No.
SMILES

CCOC1=CC2=C(C(C(C3=CC(OC)=C(C(OC)=C3)OC)=O)=C(O2)N)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Tubulin polymerization-IN-13 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tubulin polymerization-IN-132426665-56-5Microtubule/TubulinApoptosisanti-proliferative activityinduces apoptosisantivascular activityHeLaHT-29DaoyHL-60SEMJurkatInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Tubulin polymerization-IN-13
Cat. No.:
HY-146209
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.