1. Cell Cycle/DNA Damage
    Cytoskeleton
    Apoptosis
  2. Microtubule/Tubulin
    Apoptosis
  3. Tubulin polymerization-IN-13

Tubulin polymerization-IN-13 

Cat. No.: HY-146209
Handling Instructions

Tubulin polymerization-IN-13 (Compound 4f) is a tubulin polymerization inhibitor (IC50=0.37 μM). Tubulin polymerization-IN-13 shows anti-proliferative activity against cancer cells, induces apoptosis and potential antivascular activity.

For research use only. We do not sell to patients.

Tubulin polymerization-IN-13 Chemical Structure

Tubulin polymerization-IN-13 Chemical Structure

CAS No. : 2426665-56-5

Size Stock
100 mg   Get quote  
250 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Tubulin polymerization-IN-13 (Compound 4f) is a tubulin polymerization inhibitor (IC50=0.37 μM). Tubulin polymerization-IN-13 shows anti-proliferative activity against cancer cells, induces apoptosis and potential antivascular activity[1].

IC50 & Target

IC50: 0.37 μM (tubulin polymerization)[1]

In Vitro

Tubulin polymerization-IN-13 (0.005-2.8 nM) treatment inhibits tumor cell proliferation[1].
Tubulin polymerization-IN-13 (8.7-10 μM) is non-toxic in non-tumor cells[1].
Tubulin polymerization-IN-13 (1-50 nM; 24 h) treatment induces cell cycle arrest in G2/M[1].
Tubulin polymerization-IN-13 (10 nM; 24 and 48 h) treatment induces cell apoptosis[1].
Tubulin polymerization-IN-13 (10-100 nM; 24 h) treatment induces alteration of the microtubule network[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[1]

Cell Line: HeLa, HT-29, Daoy, HL-60, SEM, and Jurkat cells
Concentration: 0.005-2.8 nM
Incubation Time:
Result: Showed IC50s of 2.8 nM, 2.1 nM, 0.005 nM, 2.7 nM, 0.31 nM, and 0.28 nM for HeLa, HT-29, Daoy, HL-60, SEM, and Jurkat cells, respectively.

Cell Cytotoxicity Assay[1]

Cell Line: Peripheral blood lymphocytes (PBL)
Concentration: 8.7-10 μM
Incubation Time:
Result: Showed a GI50 of 8.7 μM in quiescent lymphocytes.

Cell Cycle Analysis[1]

Cell Line: HeLa cells
Concentration: 1, 5, 10, and 50 nM
Incubation Time: 24 hours
Result: Induced a G2/M arrest at 10 nM, increased G2/M cells accompanied by a strong reduction of cells in the G1 phase.

Apoptosis Analysis[1]

Cell Line: HeLa cells
Concentration: 10 nM
Incubation Time: 24 and 48 hours
Result: Induced caspase-9 activation, PARP cleavage, Bcl-2 phosphorylation and Mcl-1 downregulation.

Immunofluorescence[1]

Cell Line: HeLa cells
Concentration: 10, 50, and 100 nM
Incubation Time: 24 hours
Result: Showed the disorganization of microtubules at 10 nM, and much more evident at 50 and 100 nM.
In Vivo

Tubulin polymerization-IN-13 (intraperitoneal injection; 15 or 5 mg/kg; once every other day; 4 times) reduces tumor growth in a dose-dependent manner in an orthotopic murine tumor model[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Seven-week-old C57BL/6 female mice orthotopically injected into the mammary fat pad with E0771 mammary carcinoma cells[1]
Dosage: 15 or 5 mg/kg
Administration: Intraperitoneal injection; 15 or 5 mg/kg; once every other day; 4 times
Result: Reduced tumor mass by 45.7% and 16.9% at 15 and 5 mg/kg, respectively.
Showed no sign of toxicity and no decreasement in animal body weight.
Molecular Weight

371.38

Formula

C20H21NO6

CAS No.
SMILES

CCOC1=CC2=C(C(C(C3=CC(OC)=C(C(OC)=C3)OC)=O)=C(O2)N)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email address *

Phone number *

 

Organization name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Tubulin polymerization-IN-13
Cat. No.:
HY-146209
Quantity:
MCE Japan Authorized Agent: