UDP-Glc dehydrogenase (UGDH)
Based on 1 Customer Validation
UDP-Glc dehydrogenase (UGDH) catalyzes is a NAD+-dependent enzyme that catalyzes the two-fold oxidation of UDP-glucose (UDP-Glc) to produce UDP-glucuronic acid. UDP-Glc dehydrogenase (UGDH) is a key enzyme in the nucleotide-sugar interconversion pathway necessary for biosynthesis of many cell-wall polysaccharides.
For research use only. We do not sell to patients.
- Molecular Weight:57 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Product Information This product is expressed by recombinant E. coli, C-terminal His Tagged, molecular weight 57 kDa, storage buffer is 20 mM Tris-HCl, pH 8.7, 10% glycerol, mainly used to produce UDP-GlcA from UDP-Glc. Instructions 1. Add substrate according to the reaction system, 5 mM MgCl2, appropriate buffer salt (such as Tris-HCl), adjust the pH to 8.7, add appropriate amount of UGDH (according to reaction requirements); 2. After mixing, incubate the sample at 37°C for 2-4 h. Notes When the system is scaled up, the incubation time or enzyme amount needs to be adjusted according to the actual situation, and can be mixed by low-speed oscillation.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
≥1 U/mL
One unit is defined as the amount of enzyme that will catalyze the production of 1 μmol UDPglucuronic acid from UDP-glucose and NAD+ per min at 25°C
Chemical Information
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Appearance Liquid
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Molecular Weight 57 kDa
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Color Colorless to light yellow
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SMILES
[UDP-Glc dehydrogenase (UGDH)]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (264 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)