UDP-Glc dehydrogenase (UGDH)
Based on 1 Customer Validation
UDP-Glc dehydrogenase (UGDH) catalyzes is a NAD+-dependent enzyme that catalyzes the two-fold oxidation of UDP-glucose (UDP-Glc) to produce UDP-glucuronic acid. UDP-Glc dehydrogenase (UGDH) is a key enzyme in the nucleotide-sugar interconversion pathway necessary for biosynthesis of many cell-wall polysaccharides.
For research use only. We do not sell to patients.
- Molecular Weight:57 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Description
MCE Validation Data
In Vitro
Product Information This product is expressed by recombinant E. coli, C-terminal His Tagged, molecular weight 57 kDa, storage buffer is 20 mM Tris-HCl, pH 8.7, 10% glycerol, mainly used to produce UDP-GlcA from UDP-Glc. Instructions 1. Add substrate according to the reaction system, 5 mM MgCl2, appropriate buffer salt (such as Tris-HCl), adjust the pH to 8.7, add appropriate amount of UGDH (according to reaction requirements); 2. After mixing, incubate the sample at 37°C for 2-4 h. Notes When the system is scaled up, the incubation time or enzyme amount needs to be adjusted according to the actual situation, and can be mixed by low-speed oscillation.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Enzyme Activity
≥1 U/mL
Unit Definition
One unit is defined as the amount of enzyme that will catalyze the production of 1 μmol UDPglucuronic acid from UDP-glucose and NAD+ per min at 25°C
Technical Parameters
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Source
E. coli
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Tag
C-terminal His Tag
Chemical Information
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Appearance Liquid
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Molecular Weight 57 kDa
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Color Colorless to light yellow
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SMILES
[UDP-Glc dehydrogenase (UGDH)]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (264 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)