18:1 DGS-NTA(Ni)
Based on 1 Customer Validation
18:1 DGS-NTA(Ni) is a nickel chelating lipid. 18:1 DGS-NTA(Ni) has high affinity for histidine-tags and can bind histidine tags of recombinant proteins. 18:1 DGS-NTA(Ni) can be used for preparation of liposomes and nanosize multilamellar vesicles (NMVs) which are used in protein and peptide binding studies and antigen delivery.
For research use only. We do not sell to patients.
- Purity : 99%
- CAS No.: 231615-77-3
- Formula: C53H94N2NiO15
- Molecular Weight:1058.01
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
18:1 DGS-NTA(Ni) (15% of the total mass of DOPC:Cholesterol; 2-18 h) enables surface coupling of histidine-tagged rStx2B to NMVs and supports gradual antigen release while preserving antigenic integrity[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c (female, 6-8 weeks old, challenged with intravenous 1 LD100 of Stx2a)[1]
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Dosage:15% of total DOPC:Cholesterol mass (as part of NMVs loaded with 25 μg rStx2B)
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Administration:subcutaneous; three times with 2-week intervals
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Result:Increased anti-rStx2B serum IgG titers up to tenfold compared to rStx2B alone.
Achieved an Stx2-neutralization IC50 value of 365.7, significantly higher than the IC50 of 62.72 from rStx2B-only immunized mice.
Induced a mixed Th1/Th2 response with detectable IgG1 and IgG2a subclass titers.
Enabled 60% of immunized mice to survive the lethal Stx2a challenge, while 0% of rStx2B-only or non-immunized mice survived past day 3.
Chemical Information
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CAS No. 231615-77-3
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Appearance Solid
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Molecular Weight 1058.01
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Formula C53H94N2NiO15
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Color Off-white to light green
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SMILES
[OH2][Ni+2]([N](C1)(C2)C3CCCCNC(CCC(OCC(OC(CCCCCCC/C=C/CCCCCCCC)=O)COC(CCCCCCC/C=C/CCCCCCCC)=O)=O)=O)([OH]C3=O)([O-]C2=O)([O-]C1=O)[OH2]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Purity & Documentation
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Data Sheet (270 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Rodrigues-Jesus MJ, et al. Nano-multilamellar lipid vesicles (NMVs) enhance protective antibody responses against Shiga toxin (Stx2a) produced by enterohemorrhagic Escherichia coli strains (EHEC). Braz J Microbiol. 2019;50(1):67-77. [Content Brief]
[2]. Hasani M, et al. Controlled Protein-Membrane Interactions Modulate Self-Organization of Min Protein Patterns. Angew Chem Int Ed Engl. 2024;63(42):e202405046. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
18:1 DGS-NTA(Ni) Related Classifications
HY-N15871 Related Classifications
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