2-Amino benzamidoxime
Based on 1 Customer Validation
2-Amino benzamidoxime (ABAO) acts as a bioconjugation reagent precursor and a fluorescent probe precursor. 2-Amino benzamidoxime contains an aniline group for imine activation of aldehydes, as well as a nucleophilic group (Nu:) located at the ortho position of the amine, which is responsible for intramolecular cyclization. 2-Amino benzamidoxime reacts with glyoxal at the N-terminus of phage-displayed peptide libraries. Derivatives of 2-Amino benzamidoxime can be used for protein bioconjugation. Derivatives of 2-Amino benzamidoxime serve as fluorescent probes.\n
For research use only. We do not sell to patients.
- Purity: 99.85%
- CAS No.: 16348-49-5
- Formula: C7H9N3O
- Molecular Weight:151.17
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
2-Amino benzamidoxime reacts with glyoxal at the N-terminus of the phage-displayed peptide library[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 16348-49-5
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Appearance Solid
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Molecular Weight 151.17
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Formula C7H9N3O
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Color White to off-white
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SMILES
N=C(NO)C1=CC=CC=C1N
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Synonyms
ABAO
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (661.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (33.08 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (33.08 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 6.6151 mL | 33.0753 mL | 66.1507 mL | 165.3767 mL |
| 5 mM | 1.3230 mL | 6.6151 mL | 13.2301 mL | 33.0753 mL | |
| 10 mM | 0.6615 mL | 3.3075 mL | 6.6151 mL | 16.5377 mL | |
| 15 mM | 0.4410 mL | 2.2050 mL | 4.4100 mL | 11.0251 mL | |
| 20 mM | 0.3308 mL | 1.6538 mL | 3.3075 mL | 8.2688 mL | |
| 25 mM | 0.2646 mL | 1.3230 mL | 2.6460 mL | 6.6151 mL | |
| 30 mM | 0.2205 mL | 1.1025 mL | 2.2050 mL | 5.5126 mL | |
| 40 mM | 0.1654 mL | 0.8269 mL | 1.6538 mL | 4.1344 mL | |
| 50 mM | 0.1323 mL | 0.6615 mL | 1.3230 mL | 3.3075 mL | |
| 60 mM | 0.1103 mL | 0.5513 mL | 1.1025 mL | 2.7563 mL | |
| 80 mM | 0.0827 mL | 0.4134 mL | 0.8269 mL | 2.0672 mL | |
| 100 mM | 0.0662 mL | 0.3308 mL | 0.6615 mL | 1.6538 mL |
2-Amino benzamidoxime Related Classifications
HY-125372 Related Classifications
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- 2-Amino benzamidoxime
- 16348-49-5
- ABAO
- Biochemical Assay Reagents
- fluorogenic probe precursor
- aldehyde-terminated peptides
- site-specific bioconjugation
- iminium activation
- 1,2-dihydroquinazoline-3-oxide
- aldehyde-displayed glycans
- Schiff base formation
- proteins
- phage libraries
- bio-orthogonal reactivity
- Inhibitor
- inhibitor
- inhibit