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28-Homobrassinolide is a phytosteroid. 28-Homobrassinolide can be used for the research of cholesterol and glucose homeostasis.

For research use only. We do not sell to patients.

28-Homobrassinolide Chemical Structure

28-Homobrassinolide Chemical Structure

CAS No. : 82373-95-3

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10 mM * 1 mL in DMSO
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Customer Review

Based on 1 publication(s) in Google Scholar

28-Homobrassinolide Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

28-Homobrassinolide is a phytosteroid. 28-Homobrassinolide can be used for the research of cholesterol and glucose homeostasis[1].

In Vitro

28-Homobrassinolide (5-20 μM) indicates a 2-fold increase in glucose utilization and ABCA1 and SREBP2 protein expression in HepG2 cells[1].
28-Homobrassinolide decreases tissue glucose and cholesterol levels, increases cholesterol level and tissue hexokinase activity[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

28-Homobrassinolide Related Antibodies

Cell Cytotoxicity Assay[1]

Cell Line: HepG2 cells
Concentration: 1 µM, 5 µM, 10 µM, 20 µM, 30 µM, 40 µM, and 80 µM
Incubation Time: 24 h
Result: The IC50 value of 40.57µM obtained by the MTT assay.

Western Blot Analysis[1]

Cell Line: HepG2 cells
Concentration: 5, 10, and 20 μM
Incubation Time: 12 h
Result: Exhibited an increase in both ABCA1 and SREBP2 markers.
In Vivo

28-HB (1-20 μg/day; for 6 weeks) exhibites a marked decrease in aortic fat deposit and serum marker levels in high-fat diet-fed C57BL/6 mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: High-fat diet-fed C57BL/6 mice[1]
Dosage: 1-20 μg/day
Administration: For 6 weeks
Result: Observed a significant decrease in lipid deposition.
Molecular Weight

494.70

Formula

C29H50O6
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@](CC[C@]2([H])[C@H](C)[C@@H](O)[C@H](O)[C@@H](CC)C(C)C)([H])[C@@]3([H])[C@@](CC1)([H])[C@@]4([C@@](C[C@@H]([C@@H](C4)O)O)([H])C(OC3)=O)C
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 20 mg/mL (40.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0214 mL 10.1071 mL 20.2143 mL
5 mM 0.4043 mL 2.0214 mL 4.0429 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 96.96%

COA (257 KB) RP-HPLC (178 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0214 mL 10.1071 mL 20.2143 mL 50.5357 mL
5 mM 0.4043 mL 2.0214 mL 4.0429 mL 10.1071 mL
10 mM 0.2021 mL 1.0107 mL 2.0214 mL 5.0536 mL
15 mM 0.1348 mL 0.6738 mL 1.3476 mL 3.3690 mL
20 mM 0.1011 mL 0.5054 mL 1.0107 mL 2.5268 mL
25 mM 0.0809 mL 0.4043 mL 0.8086 mL 2.0214 mL
30 mM 0.0674 mL 0.3369 mL 0.6738 mL 1.6845 mL
40 mM 0.0505 mL 0.2527 mL 0.5054 mL 1.2634 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

28-Homobrassinolide82373-95-3OthersphytosteroidcholesterolglucosehomeostasisATP-binding cassette transporter (ABCA1)sterol regulatory element-binding transcription factor 2 (SREBP2)Inhibitorinhibitorinhibit

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