2. Others Apoptosis
  3. Environmental Pollutants Apoptosis
  4. Epibrassinolide

Epibrassinolide  (Synonyms: 24-Epibrassinolide; B1105; BP55)

Cat. No.: HY-N0848 Purity: 99.91%
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SDS
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Epibrassinolide (24-Epibrassinolide) is a ubiquitously occurring plant growth hormone which shows great potential to alleviate heavy metals and pesticide stress in plants. Epibrassinolide is a potential apoptotic inducer in various cancer cells without affecting the non-tumor cell growth.

For research use only. We do not sell to patients.

CAS No. : 78821-43-9

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Customer Review

Based on 16 publication(s) in Google Scholar

Other Forms of Epibrassinolide:

Epibrassinolide Related Antibodies

Top Publications Citing Use of Products

    Epibrassinolide purchased from MedChemExpress. Usage Cited in: PLoS Biol. 2025 Apr 21;23(4):e3003139.  [Abstract]

    Representative images of in vitro germinated pollen 4 h after imbibition on PGM. Imaging of in vitro pollen germination was done at 22 °C on solid PGM, containing 1.5% agarose (w/v, 10% sucrose (w/v), 0.01% boric acid (w/v), 5 mM CaCl2, 5 mM KCl, 1 mM MgSO4, adjusted to pH = 7.5 using 100 mM KOH and supplemented with 10 µM Epibrassinolide.

    Epibrassinolide purchased from MedChemExpress. Usage Cited in: Plant Stress. 2025 Jun.

    Difference in net CO2 assimilation (A) of G. humilis as affected by Epibrassinolide (EB) (2 nM) priming in each of four flooding + salinity treatments. The flooding + salinity treatments were: no flooding, low salinity with electrical conductivity of the soil solution ∼ 0 dSm-1 (F0_S0); no flooding, high salinity with electrical conductivity of the soil solution = 4 dSm-1 (F0_S4); 30 days of flooding, low salinity (F30_S0); and 30 days of flooding, high salinity (F30_S4). The EB treatments were no EB priming (E0) and EB priming (E1). Asterisks indicate significant differences among EB treatments (P ≤ 0.05).

    Epibrassinolide purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Oct 3;15(1):8565.  [Abstract]

    Effects of 2,4-epiBL (Epibrassinolide,1 μM; 2 h) on the protein stability of qRBG1 in qRBG1-GFP plants. The results showed that an exogenous Epibrassinolide treatment promoted the accumulation of qRBG1-GFP protein in the transgenic plants

    Epibrassinolide purchased from MedChemExpress. Usage Cited in: Postharvest Biol Technol. 2024 May, 211, 112832.

    Morphological changes of cut Dendrobium ‘Khao Sanan’ flowers under unstressed condition (C), water deficit for 2 d (WD), and 8 Epibrassinolide (EBL) (5 µM; 1 h) treatment before exposure to water deficit (EBL + WD) conditions during storage. Under water deficit stress, cut flowers of Dendrobium 'Khao Sanan' exhibited senescence phenotypes such as venation appearance on day 6; the senescence became aggravated with color alteration and wilting on day 9, and further developed water-soaked damage on day 12. Pretreatment with EBL markedly delayed the onset of various senescence symptoms in cut flowers under water deficit stress.

    Epibrassinolide purchased from MedChemExpress. Usage Cited in: Horticulturae. 2022, 8(12), 1115

    Survival of 'Monroe' avocado seedling trees treated with brassinosteroids (Epibrassinolide (1 nM), etc.) and either flooded or nonflooded

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Epibrassinolide (24-Epibrassinolide) is a ubiquitously occurring plant growth hormone which shows great potential to alleviate heavy metals and pesticide stress in plants[1]. Epibrassinolide is a potential apoptotic inducer in various cancer cells without affecting the non-tumor cell growth[2].

    IC50 & Target

    Plant growth regulator, Apoptosis inducer[1]
    In Vitro

    Epibrassinolide (EBR) is a biologically active compound of the brassinosteroids, steroid-derived plant growth regulator family. Cells are incubated with various doses (0-100 μM) of Epibrassinolide for 24 or 48 h and cell viability is determined by MTT assay. Epibrassinolide induced cell viability loss in dose- and time-dependent manner compared to untreated samples in LNCaP and DU145 prostate cancer cells. Increasing concentrations of Epibrassinolide is more effective on LNCaP cell viability loss than DU145 cells suggesting that androgen-dependent cells are more sensitive to Epibrassinolide than androgen-independent prostate cancer cells. In further experiments, 25 μM Epibrassinolide is selected due to its moderate cytotoxic effect on both cell lines. The effect of Epibrassinolide treatment is examined on cell proliferation by counting the cell number within 96 h. A higher and earlier inhibition of cell proliferation is observed in LNCaP than DU145 cells[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Epibrassinolide Related Antibodies
    Molecular Weight

    480.68

    Formula

    C28H48O6
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C[C@@]1([C@@]2([H])[C@H](C)[C@@H](O)[C@H](O)[C@H](C)C(C)C)[C@](CC2)([H])[C@@](COC3=O)([H])[C@]([C@@]4([C@]3([H])C[C@H](O)[C@H](O)C4)C)([H])CC1
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (208.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.0804 mL 10.4019 mL 20.8039 mL
    5 mM 0.4161 mL 2.0804 mL 4.1608 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.91%

    SDS (393 KB)

    COA (253 KB) HNMR (270 KB) RP-HPLC (239 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [1]

    LNCaP (CRL-1740) and DU145 (CRL-2698) human prostate cancer cell lines are used. PNT1a normal prostate epithelial cell line is used. Cells are grown in RPMI 1640 medium, supplemented with 10 % fetal bovine serum, 10,000 U penicillin/mL and 10 mg streptomycin/mL. Cells are cultured at 37°C in a humidified 5 % CO2 incubator (HERA cell 150). Cells are seeded overnight and then treated with the desired drug concentrations. Cells are seeded at 1×104 density per well in 96-well plates and exposed to various concentrations of Epibrassinolide for 24 and 48 h. 10 μL MTT dye (5 mg/mL) is added to each well and cells are kept at 37°C for 4 h. The resulting formazan crystals are solubilized in 200 μL DMSO. The density of the solubilized formazan is read at 570 nm spectrophotometrically[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.0804 mL 10.4019 mL 20.8039 mL 52.0097 mL
    5 mM 0.4161 mL 2.0804 mL 4.1608 mL 10.4019 mL
    10 mM 0.2080 mL 1.0402 mL 2.0804 mL 5.2010 mL
    15 mM 0.1387 mL 0.6935 mL 1.3869 mL 3.4673 mL
    20 mM 0.1040 mL 0.5201 mL 1.0402 mL 2.6005 mL
    25 mM 0.0832 mL 0.4161 mL 0.8322 mL 2.0804 mL
    30 mM 0.0693 mL 0.3467 mL 0.6935 mL 1.7337 mL
    40 mM 0.0520 mL 0.2600 mL 0.5201 mL 1.3002 mL
    50 mM 0.0416 mL 0.2080 mL 0.4161 mL 1.0402 mL
    60 mM 0.0347 mL 0.1734 mL 0.3467 mL 0.8668 mL
    80 mM 0.0260 mL 0.1300 mL 0.2600 mL 0.6501 mL
    100 mM 0.0208 mL 0.1040 mL 0.2080 mL 0.5201 mL
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    Epibrassinolide Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Epibrassinolide78821-43-924-Epibrassinolide B1105 BP55B1105B 1105B-1105BP55BP 55BP-55Environmental PollutantsApoptosisEnvironmental ContaminantsInhibitorinhibitorinhibit

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