1. Apoptosis
  2. Apoptosis
  3. Epibrassinolide

Epibrassinolide (Synonyms: 24-Epibrassinolide; B1105; BP55)

Cat. No.: HY-N0848 Purity: >98.0%
Handling Instructions

Epibrassinolide is a natural brassinosteroid (BR) derivative, is a plant regulator with a similar structure to mammalian steroids. Epibrassinolide is a potential apoptotic inducer in various cancer cells without affecting the non-tumor cell growth.

For research use only. We do not sell to patients.

Epibrassinolide Chemical Structure

Epibrassinolide Chemical Structure

CAS No. : 78821-43-9

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
5 mg USD 60 In-stock
Estimated Time of Arrival: December 31
10 mg USD 84 In-stock
Estimated Time of Arrival: December 31
50 mg USD 180 In-stock
Estimated Time of Arrival: December 31
100 mg USD 252 In-stock
Estimated Time of Arrival: December 31
500 mg USD 492 Get quote
1 g   Get quote  
5 g   Get quote  

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Other Forms of Epibrassinolide:

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Description

Epibrassinolide is a natural brassinosteroid (BR) derivative, is a plant regulator with a similar structure to mammalian steroids. Epibrassinolide is a potential apoptotic inducer in various cancer cells without affecting the non-tumor cell growth.

IC50 & Target

Plant growth regulator, Apoptosis inducer[1]

In Vitro

Epibrassinolide (EBR) is a biologically active compound of the brassinosteroids, steroid-derived plant growth regulator family. Cells are incubated with various doses (0-100 μM) of Epibrassinolide for 24 or 48 h and cell viability is determined by MTT assay. Epibrassinolide induced cell viability loss in dose- and time-dependent manner compared to untreated samples in LNCaP and DU145 prostate cancer cells. Increasing concentrations of Epibrassinolide is more effective on LNCaP cell viability loss than DU145 cells suggesting that androgen-dependent cells are more sensitive to Epibrassinolide than androgen-independent prostate cancer cells. In further experiments, 25 μM Epibrassinolide is selected due to its moderate cytotoxic effect on both cell lines. The effect of Epibrassinolide treatment is examined on cell proliferation by counting the cell number within 96 h. A higher and earlier inhibition of cell proliferation is observed in LNCaP than DU145 cells[1].

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 5 mg/mL (10.40 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0804 mL 10.4019 mL 20.8039 mL
5 mM 0.4161 mL 2.0804 mL 4.1608 mL
10 mM 0.2080 mL 1.0402 mL 2.0804 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

LNCaP (CRL-1740) and DU145 (CRL-2698) human prostate cancer cell lines are used. PNT1a normal prostate epithelial cell line is used. Cells are grown in RPMI 1640 medium, supplemented with 10 % fetal bovine serum, 10,000 U penicillin/mL and 10 mg streptomycin/mL. Cells are cultured at 37°C in a humidified 5 % CO2 incubator (HERA cell 150). Cells are seeded overnight and then treated with the desired drug concentrations. Cells are seeded at 1×104 density per well in 96-well plates and exposed to various concentrations of Epibrassinolide for 24 and 48 h. 10 μL MTT dye (5 mg/mL) is added to each well and cells are kept at 37°C for 4 h. The resulting formazan crystals are solubilized in 200 μL DMSO. The density of the solubilized formazan is read at 570 nm spectrophotometrically[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

480.68

Formula

C₂₈H₄₈O₆

CAS No.

78821-43-9

SMILES
Shipping

Room temperature in continental US; may vary elsewhere

Purity: >98.0%

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Product Name:
Epibrassinolide
Cat. No.:
HY-N0848
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Epibrassinolide

Cat. No.: HY-N0848