Epibrassinolide
Based on 16 publication(s) in Google Scholar
Epibrassinolide (24-Epibrassinolide) is a ubiquitously occurring plant growth hormone which shows great potential to alleviate heavy metals and pesticide stress in plants. Epibrassinolide is a potential apoptotic inducer in various cancer cells without affecting the non-tumor cell growth.
For research use only. We do not sell to patients.
- Purity: 99.91%
- CAS No.: 78821-43-9
- Formula: C28H48O6
- Molecular Weight:480.68
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Apoptosis Compound Library
HY-L003
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Publications Citing Use of MedChemExpress (MCE) Epibrassinolide
More- Nat Commun. 2024 Oct 3;15(1):8565. [Abstract]
- Dev Cell. 2021 May 3;56(9):1283-1295.e5. [Abstract]
- Bioresour Technol. 2018 Jul;260:124-129. [Abstract]
- Bioresour Technol. 2015 Sep;191:362-8. [Abstract]
- Plant Stress. 2025 Jun.
- Postharvest Biol Technol. 2024 May, 211, 112832.
- PLoS Biol. 2025 Apr 21;23(4):e3003139. [Abstract]
- Algal Res. 2015 Sep;11:284-293.
- Horticulturae. 2022, 8(12), 1115
- Drug Test Anal. 2021 Feb;13(2):283-298. [Abstract]
- HortScience. 11 Nov 2021.
- Chem Nat Compd. 2024 Mar 26.
- Res Sq. 2026 Jan 27.
- bioRxiv. 2024 Jan 11.
- Research Square Print. 2023 Mar 2.
- Agrosystems, Geosciences & Environment. 12 July 2021.
Customer Validation & Images
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Bio/Physico-chemical Assay
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In Vivo Imaging
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WB
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Cell Imaging/Staining
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Cell Proliferation/Viability Assay
Biological Activity
Plant growth regulator, Apoptosis inducer[1]
Epibrassinolide (EBR) is a biologically active compound of the brassinosteroids, steroid-derived plant growth regulator family. Cells are incubated with various doses (0-100 μM) of Epibrassinolide for 24 or 48 h and cell viability is determined by MTT assay. Epibrassinolide induced cell viability loss in dose- and time-dependent manner compared to untreated samples in LNCaP and DU145 prostate cancer cells. Increasing concentrations of Epibrassinolide is more effective on LNCaP cell viability loss than DU145 cells suggesting that androgen-dependent cells are more sensitive to Epibrassinolide than androgen-independent prostate cancer cells. In further experiments, 25 μM Epibrassinolide is selected due to its moderate cytotoxic effect on both cell lines. The effect of Epibrassinolide treatment is examined on cell proliferation by counting the cell number within 96 h. A higher and earlier inhibition of cell proliferation is observed in LNCaP than DU145 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 78821-43-9
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Appearance Solid
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Molecular Weight 480.68
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Formula C28H48O6
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Color White to off-white
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SMILES
C[C@@]1([C@@]2([H])[C@H](C)[C@@H](O)[C@H](O)[C@H](C)C(C)C)[C@](CC2)([H])[C@@](COC3=O)([H])[C@]([C@@]4([C@]3([H])C[C@H](O)[C@H](O)C4)C)([H])CC1
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Synonyms
24-Epibrassinolide; B1105; BP55
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (16)
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Journal Impact Factor
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Most Recent
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Nat Commun
2024 Oct 3;15(1):8565. PMID: 39362889
Epibrassinolide purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Oct 3;15(1):8565. [Abstract]
Effects of 2,4-epiBL (Epibrassinolide,1 μM; 2 h) on the protein stability of qRBG1 in qRBG1-GFP plants. The results showed that an exogenous Epibrassinolide treatment promoted the accumulation of qRBG1-GFP protein in the transgenic plants
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Dev Cell
2021 May 3;56(9):1283-1295.e5. PMID: 33831352 -
Bioresour Technol
2018 Jul;260:124-129. PMID: 29625283 -
Bioresour Technol
Identification and metabolomic analysis of chemical modulators for lipid accumulation in Crypthecodinium cohnii. [Abstract]2015 Sep;191:362-8. PMID: 25818259 -
Epibrassinolide purchased from MedChemExpress. Usage Cited in: Plant Stress. 2025 Jun.
Difference in net CO2 assimilation (A) of G. humilis as affected by Epibrassinolide (EB) (2 nM) priming in each of four flooding + salinity treatments. The flooding + salinity treatments were: no flooding, low salinity with electrical conductivity of the soil solution ∼ 0 dSm-1 (F0_S0); no flooding, high salinity with electrical conductivity of the soil solution = 4 dSm-1 (F0_S4); 30 days of flooding, low salinity (F30_S0); and 30 days of flooding, high salinity (F30_S4). The EB treatments were no EB priming (E0) and EB priming (E1). Asterisks indicate significant differences among EB treatments (P ≤ 0.05).
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Epibrassinolide purchased from MedChemExpress. Usage Cited in: Postharvest Biol Technol. 2024 May, 211, 112832.
Morphological changes of cut Dendrobium ‘Khao Sanan’ flowers under unstressed condition (C), water deficit for 2 d (WD), and 8 Epibrassinolide (EBL) (5 µM; 1 h) treatment before exposure to water deficit (EBL + WD) conditions during storage. Under water deficit stress, cut flowers of Dendrobium 'Khao Sanan' exhibited senescence phenotypes such as venation appearance on day 6; the senescence became aggravated with color alteration and wilting on day 9, and further developed water-soaked damage on day 12. Pretreatment with EBL markedly delayed the onset of various senescence symptoms in cut flowers under water deficit stress.
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PLoS Biol
Polarized subcellular activation of Rho proteins by specific ROPGEFs drives pollen germination in Arabidopsis thaliana. [Abstract]2025 Apr 21;23(4):e3003139. PMID: 40258071
Epibrassinolide purchased from MedChemExpress. Usage Cited in: PLoS Biol. 2025 Apr 21;23(4):e3003139. [Abstract]
Representative images of in vitro germinated pollen 4 h after imbibition on PGM. Imaging of in vitro pollen germination was done at 22 °C on solid PGM, containing 1.5% agarose (w/v, 10% sucrose (w/v), 0.01% boric acid (w/v), 5 mM CaCl2, 5 mM KCl, 1 mM MgSO4, adjusted to pH = 7.5 using 100 mM KOH and supplemented with 10 µM Epibrassinolide.
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Epibrassinolide purchased from MedChemExpress. Usage Cited in: Horticulturae. 2022, 8(12), 1115
Survival of 'Monroe' avocado seedling trees treated with brassinosteroids (Epibrassinolide (1 nM), etc.) and either flooded or nonflooded
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Drug Test Anal
High-throughput liquid chromatography tandem mass spectrometry assay as initial testing procedure for analysis of total urinary fraction. [Abstract]2021 Feb;13(2):283-298. PMID: 32852861 -
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Solvent & Solubility
DMSO : 100 mg/mL (208.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
LNCaP (CRL-1740) and DU145 (CRL-2698) human prostate cancer cell lines are used. PNT1a normal prostate epithelial cell line is used. Cells are grown in RPMI 1640 medium, supplemented with 10 % fetal bovine serum, 10,000 U penicillin/mL and 10 mg streptomycin/mL. Cells are cultured at 37°C in a humidified 5 % CO2 incubator (HERA cell 150). Cells are seeded overnight and then treated with the desired drug concentrations. Cells are seeded at 1×104 density per well in 96-well plates and exposed to various concentrations of Epibrassinolide for 24 and 48 h. 10 μL MTT dye (5 mg/mL) is added to each well and cells are kept at 37°C for 4 h. The resulting formazan crystals are solubilized in 200 μL DMSO. The density of the solubilized formazan is read at 570 nm spectrophotometrically[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Shahzad B, et al. Role of 24-epibrassinolide (EBL) in mediating heavy metal and pesticide induced oxidative stress in plants: A review. Ecotoxicol Environ Saf. 2018 Jan;147:935-944. [Content Brief]
[2]. Obakan P, et al. Activation of polyamine catabolic enzymes involved in diverse responses against epibrassinolide-induced apoptosis in LNCaP and DU145 prostate cancer cell lines.Amino Acids. 2014 Mar;46(3):553-64. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.0804 mL | 10.4019 mL | 20.8039 mL | 52.0097 mL |
| 5 mM | 0.4161 mL | 2.0804 mL | 4.1608 mL | 10.4019 mL | |
| 10 mM | 0.2080 mL | 1.0402 mL | 2.0804 mL | 5.2010 mL | |
| 15 mM | 0.1387 mL | 0.6935 mL | 1.3869 mL | 3.4673 mL | |
| 20 mM | 0.1040 mL | 0.5201 mL | 1.0402 mL | 2.6005 mL | |
| 25 mM | 0.0832 mL | 0.4161 mL | 0.8322 mL | 2.0804 mL | |
| 30 mM | 0.0693 mL | 0.3467 mL | 0.6935 mL | 1.7337 mL | |
| 40 mM | 0.0520 mL | 0.2600 mL | 0.5201 mL | 1.3002 mL | |
| 50 mM | 0.0416 mL | 0.2080 mL | 0.4161 mL | 1.0402 mL | |
| 60 mM | 0.0347 mL | 0.1734 mL | 0.3467 mL | 0.8668 mL | |
| 80 mM | 0.0260 mL | 0.1300 mL | 0.2600 mL | 0.6501 mL | |
| 100 mM | 0.0208 mL | 0.1040 mL | 0.2080 mL | 0.5201 mL |
Epibrassinolide Related Classifications
HY-N0848 Related Classifications
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