1. Apoptosis
  2. Apoptosis
  3. Epibrassinolide

Epibrassinolide  (Synonyms: 24-Epibrassinolide; B1105; BP55)

Cat. No.: HY-N0848 Purity: ≥98.0%
COA Handling Instructions

Epibrassinolide (24-Epibrassinolide) is a ubiquitously occurring plant growth hormone which shows great potential to alleviate heavy metals and pesticide stress in plants. Epibrassinolide is a potential apoptotic inducer in various cancer cells without affecting the non-tumor cell growth.

For research use only. We do not sell to patients.

Epibrassinolide Chemical Structure

Epibrassinolide Chemical Structure

CAS No. : 78821-43-9

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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10 mM * 1 mL in DMSO USD 73 In-stock
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50 mg USD 198 In-stock
100 mg USD 277 In-stock
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Customer Review

Based on 12 publication(s) in Google Scholar

Other Forms of Epibrassinolide:

Top Publications Citing Use of Products
  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Epibrassinolide (24-Epibrassinolide) is a ubiquitously occurring plant growth hormone which shows great potential to alleviate heavy metals and pesticide stress in plants[1]. Epibrassinolide is a potential apoptotic inducer in various cancer cells without affecting the non-tumor cell growth[2].

IC50 & Target

Plant growth regulator, Apoptosis inducer[1]

In Vitro

Epibrassinolide (EBR) is a biologically active compound of the brassinosteroids, steroid-derived plant growth regulator family. Cells are incubated with various doses (0-100 μM) of Epibrassinolide for 24 or 48 h and cell viability is determined by MTT assay. Epibrassinolide induced cell viability loss in dose- and time-dependent manner compared to untreated samples in LNCaP and DU145 prostate cancer cells. Increasing concentrations of Epibrassinolide is more effective on LNCaP cell viability loss than DU145 cells suggesting that androgen-dependent cells are more sensitive to Epibrassinolide than androgen-independent prostate cancer cells. In further experiments, 25 μM Epibrassinolide is selected due to its moderate cytotoxic effect on both cell lines. The effect of Epibrassinolide treatment is examined on cell proliferation by counting the cell number within 96 h. A higher and earlier inhibition of cell proliferation is observed in LNCaP than DU145 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

480.68

Formula

C28H48O6

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@@]1([C@@]2([H])[C@H](C)[C@@H](O)[C@H](O)[C@H](C)C(C)C)[C@](CC2)([H])[C@@](COC3=O)([H])[C@]([C@@]4([C@]3([H])C[C@H](O)[C@H](O)C4)C)([H])CC1

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (208.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0804 mL 10.4019 mL 20.8039 mL
5 mM 0.4161 mL 2.0804 mL 4.1608 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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C2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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g

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥98.0%

References
Cell Assay
[1]

LNCaP (CRL-1740) and DU145 (CRL-2698) human prostate cancer cell lines are used. PNT1a normal prostate epithelial cell line is used. Cells are grown in RPMI 1640 medium, supplemented with 10 % fetal bovine serum, 10,000 U penicillin/mL and 10 mg streptomycin/mL. Cells are cultured at 37°C in a humidified 5 % CO2 incubator (HERA cell 150). Cells are seeded overnight and then treated with the desired drug concentrations. Cells are seeded at 1×104 density per well in 96-well plates and exposed to various concentrations of Epibrassinolide for 24 and 48 h. 10 μL MTT dye (5 mg/mL) is added to each well and cells are kept at 37°C for 4 h. The resulting formazan crystals are solubilized in 200 μL DMSO. The density of the solubilized formazan is read at 570 nm spectrophotometrically[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0804 mL 10.4019 mL 20.8039 mL 52.0097 mL
5 mM 0.4161 mL 2.0804 mL 4.1608 mL 10.4019 mL
10 mM 0.2080 mL 1.0402 mL 2.0804 mL 5.2010 mL
15 mM 0.1387 mL 0.6935 mL 1.3869 mL 3.4673 mL
20 mM 0.1040 mL 0.5201 mL 1.0402 mL 2.6005 mL
25 mM 0.0832 mL 0.4161 mL 0.8322 mL 2.0804 mL
30 mM 0.0693 mL 0.3467 mL 0.6935 mL 1.7337 mL
40 mM 0.0520 mL 0.2600 mL 0.5201 mL 1.3002 mL
50 mM 0.0416 mL 0.2080 mL 0.4161 mL 1.0402 mL
60 mM 0.0347 mL 0.1734 mL 0.3467 mL 0.8668 mL
80 mM 0.0260 mL 0.1300 mL 0.2600 mL 0.6501 mL
100 mM 0.0208 mL 0.1040 mL 0.2080 mL 0.5201 mL
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Epibrassinolide
Cat. No.:
HY-N0848
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