3,5-Dibromo-4-methoxybenzoic acid (Synonyms: 3,5-Dibromo-p-anisic acid)

Cat. No.: HY-W038627 Purity: 98.56%: Data Sheet
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3,5-Dibromo-4-methoxybenzoic acid (3,5-Dibromo-p-anisic acid) is a brominated alkaloid that can be isolated from sponges such as Amphimedon sp. and Psammaplysilla purpurea.

For research use only. We do not sell to patients.

3,5-Dibromo-4-methoxybenzoic acid Chemical Structure

CAS No. : 4073-35-2

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
25 g	In-stock	Estimated Time of Arrival: December 31
50 g	Get quote

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Other Forms of 3,5-Dibromo-4-methoxybenzoic acid:

3,5-Dibromo-4-methoxybenzoic acid (Standard) Get quote

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Description

3,5-Dibromo-4-methoxybenzoic acid (3,5-Dibromo-p-anisic acid) is a brominated alkaloid that can be isolated from sponges such as Amphimedon sp. and Psammaplysilla purpurea^[1]^[2].

Molecular Weight

309.94

Formula

C₈H₆Br₂O₃

CAS No.

4073-35-2

Appearance

Solid

Color

White to off-white

SMILES

O=C(O)C1=CC(Br)=C(OC)C(Br)=C1

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Animals

Amphimedon sp.

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (322.64 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2264 mL	16.1321 mL	32.2642 mL
5 mM	0.6453 mL	3.2264 mL	6.4528 mL
10 mM	0.3226 mL	1.6132 mL	3.2264 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (8.07 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.54%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (249 KB) HNMR (130 KB) RP-HPLC (193 KB)

Handling Instructions (2659 KB)

References

[1]. Campos P E, et al. Amphimedonoic acid and psammaplysene E, novel brominated alkaloids from Amphimedon sp[J]. Tetrahedron Letters, 2017, 58(40): 3901-3904.

[2]. Kallscheuer N, et al. The bacterial phylum Planctomycetes as novel source for bioactive small molecules[J]. Biotechnology advances, 2021, 53: 107818. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2264 mL	16.1321 mL	32.2642 mL	80.6605 mL
	5 mM	0.6453 mL	3.2264 mL	6.4528 mL	16.1321 mL
	10 mM	0.3226 mL	1.6132 mL	3.2264 mL	8.0661 mL
	15 mM	0.2151 mL	1.0755 mL	2.1509 mL	5.3774 mL
	20 mM	0.1613 mL	0.8066 mL	1.6132 mL	4.0330 mL
	25 mM	0.1291 mL	0.6453 mL	1.2906 mL	3.2264 mL
	30 mM	0.1075 mL	0.5377 mL	1.0755 mL	2.6887 mL
	40 mM	0.0807 mL	0.4033 mL	0.8066 mL	2.0165 mL
	50 mM	0.0645 mL	0.3226 mL	0.6453 mL	1.6132 mL
	60 mM	0.0538 mL	0.2689 mL	0.5377 mL	1.3443 mL
	80 mM	0.0403 mL	0.2017 mL	0.4033 mL	1.0083 mL
	100 mM	0.0323 mL	0.1613 mL	0.3226 mL	0.8066 mL

3,5-Dibromo-4-methoxybenzoic acid Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

3,5-Dibromo-4-methoxybenzoic acid4073-35-23,5-Dibromo-p-anisic acidOtherscarcinoma KB cellsspongesAmphimedon sp.Psammaplysilla purpureaInhibitorinhibitorinhibit

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3,5-Dibromo-4-methoxybenzoic acid (Synonyms: 3,5-Dibromo-p-anisic acid)

Other Forms of 3,5-Dibromo-4-methoxybenzoic acid:

3,5-Dibromo-4-methoxybenzoic acid Related Antibodies

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•Related Screening Libraries:

•Related Small Molecules:

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Biological Activity

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References

Customer Review

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