5-Carboxytetramethylrhodamine

Cat. No.: HY-D0941 Purity: 99.13%: Data Sheet
COA

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5-Carboxytetramethylrhodamine can be used as a fluorescent probe of nucleic acids and proteins. 5-Carboxytetramethylrhodamine displays excitation maxima of 558 nm and an emission maximum of 586 nm.

For research use only. We do not sell to patients.

5-Carboxytetramethylrhodamine Chemical Structure

CAS No. : 150322-05-7

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE 5-Carboxytetramethylrhodamine

•Int Immunopharmacol. 2025 Dec 23:170:116097. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

5-Carboxytetramethylrhodamine can be used as a fluorescent probe of nucleic acids and proteins. 5-Carboxytetramethylrhodamine displays excitation maxima of 558 nm and an emission maximum of 586 nm^[1]^[2]^[3].

Molecular Weight

430.45

Formula

C₂₅H₂₂N₂O₅

CAS No.

150322-05-7

Appearance

Solid

Color

Brown to breen

SMILES

O=C(C1=CC2=C(C3(C4=C(OC5=C3C=CC(N(C)C)=C5)C=C(N(C)C)C=C4)OC2=O)C=C1)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 11.67 mg/mL (27.11 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3232 mL	11.6158 mL	23.2315 mL
5 mM	0.4646 mL	2.3232 mL	4.6463 mL
10 mM	0.2323 mL	1.1616 mL	2.3232 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.13%

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Data Sheet (278 KB) SDS (252 KB)

COA (258 KB) HNMR (266 KB) LCMS (90 KB)

Handling Instructions (2659 KB)

References

[1]. Lyu Z, et al. Steric-Free Bioorthogonal Labeling of Acetylation Substrates Based on a Fluorine-Thiol Displacement Reaction. J Am Chem Soc. 2021 Jan 27;143(3):1341-1347. [Content Brief]

[2]. Bucevičius J, et al. Rhodamine-Hoechst positional isomers for highly efficient staining of heterochromatin. Chem Sci. 2018 Dec 12;10(7):1962-1970. [Content Brief]

[3]. Lyttle MH, et al. A tetramethyl rhodamine (Tamra) phosphoramidite facilitates solid-phase-supported synthesis of 5'-Tamra DNA. J Org Chem. 2000 Dec 29;65(26):9033-8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3232 mL	11.6158 mL	23.2315 mL	58.0788 mL
	5 mM	0.4646 mL	2.3232 mL	4.6463 mL	11.6158 mL
	10 mM	0.2323 mL	1.1616 mL	2.3232 mL	5.8079 mL
	15 mM	0.1549 mL	0.7744 mL	1.5488 mL	3.8719 mL
	20 mM	0.1162 mL	0.5808 mL	1.1616 mL	2.9039 mL
	25 mM	0.0929 mL	0.4646 mL	0.9293 mL	2.3232 mL