5-Carboxytetramethylrhodamine
Based on 1 publication(s) in Google Scholar
5-Carboxytetramethylrhodamine can be used as a fluorescent probe of nucleic acids and proteins. 5-Carboxytetramethylrhodamine displays excitation maxima of 558 nm and an emission maximum of 586 nm.
For research use only. We do not sell to patients.
- Purity: 99.13%
- CAS No.: 150322-05-7
- Formula: C25H22N2O5
- Molecular Weight:430.45
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications Citing Use of MedChemExpress (MCE) 5-Carboxytetramethylrhodamine
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Biological Activity
Chemical Information
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CAS No. 150322-05-7
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Appearance Solid
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Molecular Weight 430.45
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Formula C25H22N2O5
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Color Brown to breen
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SMILES
O=C(C1=CC2=C(C3(C4=C(OC5=C3C=CC(N(C)C)=C5)C=C(N(C)C)C=C4)OC2=O)C=C1)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
DMSO : 11.67 mg/mL (27.11 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Lyu Z, et al. Steric-Free Bioorthogonal Labeling of Acetylation Substrates Based on a Fluorine-Thiol Displacement Reaction. J Am Chem Soc. 2021 Jan 27;143(3):1341-1347. [Content Brief]
[2]. Bucevičius J, et al. Rhodamine-Hoechst positional isomers for highly efficient staining of heterochromatin. Chem Sci. 2018 Dec 12;10(7):1962-1970. [Content Brief]
[3]. Lyttle MH, et al. A tetramethyl rhodamine (Tamra) phosphoramidite facilitates solid-phase-supported synthesis of 5'-Tamra DNA. J Org Chem. 2000 Dec 29;65(26):9033-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3232 mL | 11.6158 mL | 23.2315 mL | 58.0788 mL |
| 5 mM | 0.4646 mL | 2.3232 mL | 4.6463 mL | 11.6158 mL | |
| 10 mM | 0.2323 mL | 1.1616 mL | 2.3232 mL | 5.8079 mL | |
| 15 mM | 0.1549 mL | 0.7744 mL | 1.5488 mL | 3.8719 mL | |
| 20 mM | 0.1162 mL | 0.5808 mL | 1.1616 mL | 2.9039 mL | |
| 25 mM | 0.0929 mL | 0.4646 mL | 0.9293 mL | 2.3232 mL |