6-Aza-UMP disodium
Based on 1 Customer Validation
6-Aza-UMP disodium is a competitive inhibitor of the OMP decarboxylase domain of UMP synthase (UMP synthase), with a Ki value of 0.51 μM against OMP decarboxylase. 6-Aza-UMP disodium reduces the production of UMP and increases the abundance of OMP. 6-Aza-UMP disodium promotes the formation of toxic 6-aza-UTP, thereby exerting cytotoxicity against bacterial. 6-Aza-UMP disodium can be used in studies related to bacterial infections.
For research use only. We do not sell to patients.
- Formula: C8H10N3Na2O9P
- Molecular Weight:369.13
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
6-Aza-UMP (10-250 μM; 20 min) disodium potently inhibits the activity of wild-type human UMPS in vitro, and a significant reduction in UMP production is observed at concentrations as low as 10 μM[1].
6-Aza-UMP (0.02-2.0 mM; duration of the spectrophotometric absorbance recording) disodium serves as a substrate for UMP kinases from Bacillus subtilis and Escherichia coli[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 369.13
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Formula C8H10N3Na2O9P
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SMILES
O=C(C=NN1[C@H]2[C@H](O)[C@H](O)[C@@H](COP(O[Na])(O[Na])=O)O2)NC1=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
H2O : 62.5 mg/mL (169.32 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
References
[1]. Cantor JR, et al. Physiologic Medium Rewires Cellular Metabolism and Reveals Uric Acid as an Endogenous Inhibitor of UMP Synthase. Cell. 2017 Apr 06;169(2):258-272.e17. [Content Brief]
[2]. Gagyi C, et al. UMP kinase from the Gram-positive bacterium Bacillus subtilis is strongly dependent on GTP for optimal activity. European journal of biochemistry. 2003 Aug;270(15):3196-204. [Content Brief]
[3]. Wu N, et al. Purification, crystallization and preliminary X-ray study of orotidine 5'-monophosphate decarboxylase. Acta crystallographica. Section D, Biological crystallography. 2000 Jul;56(Pt 7):912-4. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.7091 mL | 13.5454 mL | 27.0907 mL | 67.7268 mL |
| 5 mM | 0.5418 mL | 2.7091 mL | 5.4181 mL | 13.5454 mL | |
| 10 mM | 0.2709 mL | 1.3545 mL | 2.7091 mL | 6.7727 mL | |
| 15 mM | 0.1806 mL | 0.9030 mL | 1.8060 mL | 4.5151 mL | |
| 20 mM | 0.1355 mL | 0.6773 mL | 1.3545 mL | 3.3863 mL | |
| 25 mM | 0.1084 mL | 0.5418 mL | 1.0836 mL | 2.7091 mL | |
| 30 mM | 0.0903 mL | 0.4515 mL | 0.9030 mL | 2.2576 mL | |
| 40 mM | 0.0677 mL | 0.3386 mL | 0.6773 mL | 1.6932 mL | |
| 50 mM | 0.0542 mL | 0.2709 mL | 0.5418 mL | 1.3545 mL | |
| 60 mM | 0.0452 mL | 0.2258 mL | 0.4515 mL | 1.1288 mL | |
| 80 mM | 0.0339 mL | 0.1693 mL | 0.3386 mL | 0.8466 mL | |
| 100 mM | 0.0271 mL | 0.1355 mL | 0.2709 mL | 0.6773 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
6-Aza-UMP disodium Related Classifications
HY-W826156A Related Classifications
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