7-Hydroxycoumarin-4-acetic acid

Cat. No.: HY-D0112 Purity: 98.71%: Data Sheet
COA

SDS
Handling Instructions
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7-Hydroxycoumarin-4-acetic acid, a pH-indicator dye, is covalently bound to bovine serum albumin (BSA) at the positions of reactive amino groups (λ_max=326 nm).

For research use only. We do not sell to patients.

7-Hydroxycoumarin-4-acetic acid Chemical Structure

CAS No. : 6950-82-9

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
250 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
50 g	Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

7-Hydroxycoumarin-4-acetic acid, a pH-indicator dye, is covalently bound to bovine serum albumin (BSA) at the positions of reactive amino groups (λ_max=326 nm)^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
B16-F10	IC₅₀	>10 μM Compound: HCAA	Cytotoxicity against mouse B16-F10 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Cytotoxicity against mouse B16-F10 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 33956447]
HepG2	IC₅₀	>10 μM Compound: HCAA	Cytotoxicity against human HepG2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 33956447]
HT-29	IC₅₀	>10 μM Compound: HCAA	Cytotoxicity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Cytotoxicity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 33956447]

Molecular Weight

220.18

Formula

C₁₁H₈O₅

CAS No.

6950-82-9

Appearance

Solid

Color

White to off-white

Emission (Em)

450

Excitation (Ex)

415

SMILES

O=C(O)CC(C1=CC=C(O)C=C1O2)=CC2=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (454.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.5417 mL	22.7087 mL	45.4174 mL
5 mM	0.9083 mL	4.5417 mL	9.0835 mL
10 mM	0.4542 mL	2.2709 mL	4.5417 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

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Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.71%

Select Batch:

Data Sheet (270 KB) SDS (394 KB)

COA (250 KB) HNMR (130 KB) RP-HPLC (226 KB)

Handling Instructions (2659 KB)

References

[1]. Umek T, et al. Oligonucleotide Binding to Non-B-DNA in MYC. Molecules. 2019 Mar 12;24(5):1000. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.5417 mL	22.7087 mL	45.4174 mL	113.5435 mL
	5 mM	0.9083 mL	4.5417 mL	9.0835 mL	22.7087 mL
	10 mM	0.4542 mL	2.2709 mL	4.5417 mL	11.3543 mL
	15 mM	0.3028 mL	1.5139 mL	3.0278 mL	7.5696 mL
	20 mM	0.2271 mL	1.1354 mL	2.2709 mL	5.6772 mL
	25 mM	0.1817 mL	0.9083 mL	1.8167 mL	4.5417 mL
	30 mM	0.1514 mL	0.7570 mL	1.5139 mL	3.7848 mL
	40 mM	0.1135 mL	0.5677 mL	1.1354 mL	2.8386 mL
	50 mM	0.0908 mL	0.4542 mL	0.9083 mL	2.2709 mL
	60 mM	0.0757 mL	0.3785 mL	0.7570 mL	1.8924 mL
	80 mM	0.0568 mL	0.2839 mL	0.5677 mL	1.4193 mL
	100 mM	0.0454 mL	0.2271 mL	0.4542 mL	1.1354 mL