7-Hydroxycoumarin-4-acetic acid
Based on 1 Customer Validation
7-Hydroxycoumarin-4-acetic acid, a pH-indicator dye, is covalently bound to bovine serum albumin (BSA) at the positions of reactive amino groups (λmax=326 nm).
For research use only. We do not sell to patients.
- Purity: 98.71%
- CAS No.: 6950-82-9
- Formula: C11H8O5
- Molecular Weight:220.18
-
Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Biological Activity
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| B16-F10 | IC50 |
>10 μM
Compound: HCAA
|
Cytotoxicity against mouse B16-F10 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
Cytotoxicity against mouse B16-F10 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
|
[PMID: 33956447] |
| HepG2 | IC50 |
>10 μM
Compound: HCAA
|
Cytotoxicity against human HepG2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
Cytotoxicity against human HepG2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
|
[PMID: 33956447] |
| HT-29 | IC50 |
>10 μM
Compound: HCAA
|
Cytotoxicity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
Cytotoxicity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
|
[PMID: 33956447] |
450
415
Chemical Information
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CAS No. 6950-82-9
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Appearance Solid
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Molecular Weight 220.18
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Formula C11H8O5
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Color White to off-white
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SMILES
O=C(O)CC(C1=CC=C(O)C=C1O2)=CC2=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (454.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (270 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.5417 mL | 22.7087 mL | 45.4174 mL | 113.5435 mL |
| 5 mM | 0.9083 mL | 4.5417 mL | 9.0835 mL | 22.7087 mL | |
| 10 mM | 0.4542 mL | 2.2709 mL | 4.5417 mL | 11.3543 mL | |
| 15 mM | 0.3028 mL | 1.5139 mL | 3.0278 mL | 7.5696 mL | |
| 20 mM | 0.2271 mL | 1.1354 mL | 2.2709 mL | 5.6772 mL | |
| 25 mM | 0.1817 mL | 0.9083 mL | 1.8167 mL | 4.5417 mL | |
| 30 mM | 0.1514 mL | 0.7570 mL | 1.5139 mL | 3.7848 mL | |
| 40 mM | 0.1135 mL | 0.5677 mL | 1.1354 mL | 2.8386 mL | |
| 50 mM | 0.0908 mL | 0.4542 mL | 0.9083 mL | 2.2709 mL | |
| 60 mM | 0.0757 mL | 0.3785 mL | 0.7570 mL | 1.8924 mL | |
| 80 mM | 0.0568 mL | 0.2839 mL | 0.5677 mL | 1.4193 mL | |
| 100 mM | 0.0454 mL | 0.2271 mL | 0.4542 mL | 1.1354 mL |