8-Bromo-ATP
8-Bromo-ATP (8-Bromoadenosine 5'-triphosphate), an ATP analogue, is a purinergic P2X receptor agonist. 8-Bromo-ATP shows cytotoxic to multiple myeloma cells with an IC50 of 23.1 μM.
For research use only. We do not sell to patients.
- CAS No.: 23567-97-7
- Formula: C10H15BrN5O13P3
- Molecular Weight:586.08
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
8-Bromo-ATP (10-50 μM; 5 days) treatment shows cytotoxic to multiple myeloma[3].
Fluorescence measurements are made possible through the use of 8-Bromo-ATP, which selectively quenched certain tryptophan residues of the ATPase. 8-Bromo-ATP enhances the rate of dephosphorylation of native ATPase 2-3-fold when added in the absence of divalent cations[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:MM 1.s cells
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Concentration:10 μM, 20 μM, 30 μM, 40 μM, 50 μM
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Incubation Time:5 days
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Result:Showed cytotoxic to multiple myeloma.
Chemical Information
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CAS No. 23567-97-7
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Molecular Weight 586.08
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Formula C10H15BrN5O13P3
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SMILES
O=P(O)(O)OP(OP(OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(Br)=NC3=C(N)N=CN=C23)O1)(O)=O)(O)=O
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Synonyms
8-Bromoadenosine 5'-triphosphate; 8-Br-ATP
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
[2]. P Champeil, et al. ATP regulation of sarcoplasmic reticulum Ca2+-ATPase. Metal-free ATP and 8-bromo-ATP bind with high affinity to the catalytic site of phosphorylated ATPase and accelerate dephosphorylation. J Biol Chem. 1988 Sep 5;263(25):12288-94. [Content Brief]
[3]. Li Wang, et al. Cationic phospholiposomes: efficient delivery vehicles of anticancer derivatives of ATP to multiple myeloma cells. J Liposome Res. 2011 Dec;21(4):306-14. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
8-Bromo-ATP Related Classifications
HY-134262 Related Classifications
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