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  3. Aconine

Aconine (Synonyms: Jesaconine)

Cat. No.: HY-N0277 Purity: 99.23%
Handling Instructions

Aconine inhibits receptor activator of nuclear factor (NF)-κB ligand (RANKL)-induced NF-κB activation.

For research use only. We do not sell to patients.

Aconine Chemical Structure

Aconine Chemical Structure

CAS No. : 509-20-6

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10 mM * 1 mL in DMSO USD 244 In-stock
Estimated Time of Arrival: December 31
5 mg USD 222 In-stock
Estimated Time of Arrival: December 31
10 mg USD 306 In-stock
Estimated Time of Arrival: December 31
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Description

Aconine inhibits receptor activator of nuclear factor (NF)-κB ligand (RANKL)-induced NF-κB activation.

IC50 & Target

NF-κB

 

In Vitro

Treatment with Aconine significantly inhibits the RANKL-induced transcriptional activity of NF-κB in a dose-dependent manner. Aconine inhibits RANKL-induced osteoclast differentiation in RAW264.7 cells by suppressing the activation of NF-κB and NFATc1 and the expression of the cell-cell fusion molecule DC-STAMP. Aconine (0.125, 0.25 μM) does not affect the viability of RAW264.7 cells, but dose-dependently inhibits RANKL-induced osteoclast formation and bone resorptive activity. Aconine dose-dependently inhibits the RANKL-induced activation of NF-κB and NFATc1 in RAW264.7 cells, and subsequently reduces the expression of osteoclast-specific genes (c-Src, β3-Integrin, cathepsin K and MMP-9) and the expression of dendritic cell-specific transmembrane protein (DC-STAMP), which plays an important role in cell-cell fusion[1].

Molecular Weight

499.59

Formula

C₂₅H₄₁NO₉

CAS No.

509-20-6

SMILES
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 125 mg/mL (250.21 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0016 mL 10.0082 mL 20.0164 mL
5 mM 0.4003 mL 2.0016 mL 4.0033 mL
10 mM 0.2002 mL 1.0008 mL 2.0016 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (4.16 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (4.16 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (4.16 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[1]

To evaluate the effect of Aconine on the viability of RAW264.7 cells, cytotoxicity assays are performed using the Cell Counting Kit-8. Briefly, the cells are seeded in 96-well plates at a density of 2×104, 3×103, 1.2×103, 1×103 or 1×103 cells/well in the presence or absence of Aconine (0.125-0.5 mM) for 8 h, 24 h, 48 h, 5 d or 7 d, respectively. After incubating the cells with CCK-8 solution for 2 h, optical density is measured at 450 nm using a GENios microplate reader. Cell viability is expressed as a percentage of the control[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Keywords:

AconineJesaconineNF-κBNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

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Aconine
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