BIX-01294
Based on 27 publication(s) in Google Scholar
BIX-01294 is a reversible and highly selective G9a and GLP Histone Methyltransferase inhibitor, with IC50s of of 1.7 μM and 0.9 μM, respectively. BIX-01294 inhibits G9a/GLP by competing for binding with the amino acids N-terminal of the substrate lysine residue. BIX-01294, a (1H-1,4-diazepin-1-yl)-quinazolin-4-yl amine derivative, induces necroptosis and autophagy. BIX-01294 has antitumor activity in recurrent tumor cells.
For research use only. We do not sell to patients.
- Purity: 98.44%
- CAS No.: 935693-62-2
- Formula: C28H38N6O2
- Molecular Weight:490.64
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
Publications Citing Use of MedChemExpress (MCE) BIX-01294
More- Nat Microbiol. 2025 Oct 10. [Abstract]
- ACS Nano. 2023 Feb 14;17(3):3181-3193. [Abstract]
- Adv Sci (Weinh). 2024 Jul 19:e2309983. [Abstract]
- J Exp Clin Cancer Res. 2018 Aug 17;37(1):196. [Abstract]
- Cell Death Dis. 2019 Apr 15;10(5):331. [Abstract]
- Cell Death Dis. 2017 Apr 6;8(4):e2726. [Abstract]
- Cell Syst. 2018 Apr 25;6(4):424-443.e7. [Abstract]
- NPJ Regen Med. 2024 Apr 29;9(1):17. [Abstract]
- Biomolecules. 2026 Feb 25;16(3):345. [Abstract]
- Molecules. 2025 Jul 22;30(15):3054. [Abstract]
- J Cell Mol Med. 2022 Nov;26(21):5539-5550. [Abstract]
- iScience. 2021 Oct 14;24(11):103271. [Abstract]
- Aging (Albany NY). 2021 Mar 19;13(7):9704-9718. [Abstract]
- Biomedicines. 2020 Nov 9;8(11):485. [Abstract]
- Biochim Biophys Acta. 2018 May;1864(5 Pt A):1744-1753. [Abstract]
- Mol Med Rep. 2023 Feb;27(2):21. [Abstract]
- Mol Med Rep. 2021 Aug;24(2):616. [Abstract]
- Exp Cell Res. 2020 Aug 15;393(2):112090. [Abstract]
- Invest New Drugs. 2021 Jun;39(3):686-696. [Abstract]
- PLoS One. 2023 Aug 29;18(8):e0289510. [Abstract]
- Biochem Biophys Res Commun. 2025 Nov 29:794:153072. [Abstract]
- Biochem Biophys Res Commun. 2025 Jan:743:151171. [Abstract]
- Cell J. 2023 Feb 1;25(2):118-125. [Abstract]
- bioRxiv. 2024 Oct 30:2024.09.30.615828. [Abstract]
- Research Square Print. September 20th, 2022.
- bioRxiv. 2020 Jun.
- Patent. US20180263995A1.
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Bio/Physico-chemical Assay
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Cell Proliferation/Viability Assay
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ELISA
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RT-PCR
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WB
All Histone Methyltransferase Isoforms
More
Biological Activity
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EHMT2/G9a/KMT1C |
EHMT1/GLP/KMT1D |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
2.8 μM
Compound: BIX01294
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Antiproliferative activity against human A549 cells after 48 hrs by CCK8 assay
Antiproliferative activity against human A549 cells after 48 hrs by CCK8 assay
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[PMID: 27393948] |
| HEK293 | IC50 |
2.05 μM
Compound: 38; BIX01294
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Cytotoxicity against HEK293 cells
Cytotoxicity against HEK293 cells
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[PMID: 34107385] |
| HEK293 | IC50 |
2.05 μM
Compound: BIXO1294
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Cytotoxicity HEK293 cells assessed as inhibition of cell growth incubated for 72 hrs
Cytotoxicity HEK293 cells assessed as inhibition of cell growth incubated for 72 hrs
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[PMID: 38056296] |
| HeLa | IC50 |
0.966 μM
Compound: BIX-01294
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Inhibition of Ebolavirus glycoprotein/matrix protein VP40 entry in human HeLa cells after 4.5 hrs beta-lactamase reporter assay
Inhibition of Ebolavirus glycoprotein/matrix protein VP40 entry in human HeLa cells after 4.5 hrs beta-lactamase reporter assay
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[PMID: 29624387] |
| HepG2 | IC50 |
3.7 μM
Compound: BIX01294
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Antiproliferative activity against human HepG2 cells after 48 hrs by CCK8 assay
Antiproliferative activity against human HepG2 cells after 48 hrs by CCK8 assay
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[PMID: 27393948] |
| HepG2 | IC50 |
4800 nM
Compound: BIX01294; 1
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Cytotoxicity against human HepG2 cells after 3 days by CellTiter blue assay
Cytotoxicity against human HepG2 cells after 3 days by CellTiter blue assay
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[PMID: 29308121] |
| HFF | IC50 |
4997 nM
Compound: 18; BIX01294
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Cytotoxicity against HFF
Cytotoxicity against HFF
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[PMID: 30366254] |
| HL-60 | IC50 |
2.2 μM
Compound: BIX01294
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Antiproliferative activity against human HL60 cells after 48 hrs by CCK8 assay
Antiproliferative activity against human HL60 cells after 48 hrs by CCK8 assay
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[PMID: 27393948] |
| K562 | IC50 |
3.9 μM
Compound: BIX01294
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Antiproliferative activity against human K562 cells after 48 hrs by CCK8 assay
Antiproliferative activity against human K562 cells after 48 hrs by CCK8 assay
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[PMID: 27393948] |
| L02 | IC50 |
4.28 μM
Compound: BIX-01294
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Cytotoxicity against human L02 cells assessed as reduction in cell viability incubated for 2 days by MTT assay
Cytotoxicity against human L02 cells assessed as reduction in cell viability incubated for 2 days by MTT assay
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[PMID: 37788550] |
| MCF7 | EC50 |
3.3 μM
Compound: 1; BIX01294
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Antiproliferative activity against human MCF7 cells incubated for 72 hrs by MTT assay
Antiproliferative activity against human MCF7 cells incubated for 72 hrs by MTT assay
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[PMID: 31627059] |
| MDA-MB-231 | EC50 |
2.9 μM
Compound: 1; BIX01294
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Antiproliferative activity against human MDA-MB-231 cells incubated for 72 hrs by MTT assay
Antiproliferative activity against human MDA-MB-231 cells incubated for 72 hrs by MTT assay
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[PMID: 31627059] |
| MDA-MB-231 | EC50 |
2700 nM
Compound: 2
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Antiproliferative activity against human MDA-MB-231 cells by MTT assay
Antiproliferative activity against human MDA-MB-231 cells by MTT assay
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[PMID: 38799223] |
| MDA-MB-231 | IC50 |
500 nM
Compound: 2
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Downregulation of H3K9me2 level in human MDA-MB-231 cells measured after 48 hrs by Western blot analysis
Downregulation of H3K9me2 level in human MDA-MB-231 cells measured after 48 hrs by Western blot analysis
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[PMID: 38799223] |
| MDA-MB-231 | IC50 |
500 nM
Compound: 37; BIX01294
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Reduction in H3K9me2 level in human MDA-MB-231 cells incubated for 48 hrs by ChIP assay
Reduction in H3K9me2 level in human MDA-MB-231 cells incubated for 48 hrs by ChIP assay
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[PMID: 36528996] |
| MDA-MB-231 | IC50 |
9.6 μM
Compound: 133; BIX-01294
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Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell viability incubated for 72 hrs by MTT assay
Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell viability incubated for 72 hrs by MTT assay
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[PMID: 35531606] |
| Vero C1008 | IC50 |
1 μM
Compound: BIX-01294
|
Antiviral activity against Ebolavirus Mayinga infected in African green monkey Vero E6 cells after 72 hrs by eGFP assay
Antiviral activity against Ebolavirus Mayinga infected in African green monkey Vero E6 cells after 72 hrs by eGFP assay
|
[PMID: 29624387] |
BIX-01294 (2 μM; 48 h) selectively inhibits recurrent tumor cell growth[1].
BIX-01294 (1 μM) leads to a marked increase in phosphorylation of S345 of MLKL[1].
BIX-01294 (1 μM) significantly upregulates the canonical p53 targets Cdkn1a (p21) and Gadd45a in recurrent tumor cell lines[1].
BIX-01294 (1 μM; 6 days) causes the reduction in H3K9me2 levels in primary and recurrent tumor cells[1].
BIX-01294 leads to necroptotic cell death in recurrent tumor cells. Necrostatin-1 (30 μM) partially reverses cell death induced by BIX-01294 (750 nM; 24 h)[1].
BIX-01294 (4.1 μM; for 2 days) causes around a 20% reduction, concomitant with a comparable increase in the unmodified H3K9 fragment in H3K9me2 in mouse ES cells. BIX-01294 causes pronounced reduction in H3K9me2 and a small decrease for H3K9me3 and H3K9me1 in wild-type ES cells[2].
BIX-01294 has no inhibition of the other histone methyltransferases even at concentrations of 45 μM. BIX-01294 does not affect SUV39H1 (H320R) and PRMT1 within the tested concentration range (up to 10 μM)[2].
BIX-01294 inhibits G9a in an uncompetitive manner with S-adenosyl-methionine (SAM)[2].
BIX-01294 (1 μg/mL) causes reduction in the BrdU incorporation of fetal PASMCs. BIX-01294 treatment decreases the PASMCs migration induced by PDGF[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Primary or recurrent tumor cells
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Concentration:2 μM
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Incubation Time:48 hours
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Result:Selectively inhibited recurrent tumor cell growth.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Female MMTV-rtTA;TetO-Her2/neu (MTB;TAN) and TetO-Her2/neu (TAN) mice with recurrent or primary tumor cells[1]
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Dosage:10 mg/kg
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Administration:IP; three times a week for 2 weeks
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Result:Significantly reduced tumor growth and tumor burden in recurrent tumor cells.
Primary tumor growth was not inhibited.
Slowed the growth of orthotopic recurrent tumors in athymic nude recipients.
Chemical Information
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CAS No. 935693-62-2
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Appearance Solid
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Molecular Weight 490.64
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Formula C28H38N6O2
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Color White to off-white
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SMILES
COC1=C(OC)C=C(C(NC2CCN(CC3=CC=CC=C3)CC2)=NC(N4CCN(C)CCC4)=N5)C5=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (27)
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Journal Impact Factor
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Most Recent
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Nat Microbiol
2025 Oct 10. PMID: 41073665
BIX-01294 purchased from MedChemExpress. Usage Cited in: Nat Microbiol. 2025 Oct 10. [Abstract]
Both KL1 (40, 100 µM) and BIX-01294 (1, 4 µM) reduced ROS levels at 4 hpi. Chemiluminescent L-012 probes were used to quantify reactive species.
BIX-01294 purchased from MedChemExpress. Usage Cited in: Nat Microbiol. 2025 Oct 10. [Abstract]
Inhibition of EHMT2/G9a phenocopied KL1-mediated sensitization in intracellular S. aureus. A selective EHMT2/G9a inhibitor, BIX-01294 (BIX; 1–10 µM), increased the bioluminescence signal (grey bars) of strain JE2-lux compared to the vehicle control (Veh; 0.1% DMSO). Cytotoxicity of BIX was also assessed (red circles). Representative data of 3 experiments (n = 6, two-sided unpaired t-test) were obtained.
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ACS Nano
Binary Colloidal Crystals Promote Cardiac Differentiation of Human Pluripotent Stem Cells via Nuclear Accumulation of SETDB1. [Abstract]2023 Feb 14;17(3):3181-3193. PMID: 36655945 -
Adv Sci (Weinh)
KDM3A Ablation Activates Endogenous Retrovirus Expression to Stimulate Antitumor Immunity in Gastric Cancer. [Abstract]2024 Jul 19:e2309983. PMID: 39031630 -
J Exp Clin Cancer Res
2018 Aug 17;37(1):196. PMID: 30119635
BIX-01294 purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2018 Aug 17;37(1):196. [Abstract]
The relative mRNA levels of Ki67 and PCNA in primary CRC cells responding to rhIL-33 (100 ng/mL) incubation and/ or indicated inhibitors (SB203580, 10 μg/mL; PD98059, 20 μg/mL; SP600125, 10 μg/mL; BIX01294, 2 μM; 5Aza, 10 μM; SC560, 0.1 μg/mL; celecoxib, 20 μg/mL) for 24 h.
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Cell Death Dis
Ferritinophagy is required for the induction of ferroptosis by the bromodomain protein BRD4 inhibitor (+)-JQ1 in cancer cells. [Abstract]2019 Apr 15;10(5):331. PMID: 30988278 -
Cell Death Dis
G9A promotes tumor cell growth and invasion by silencing CASP1 in non-small-cell lung cancer cells. [Abstract]2017 Apr 6;8(4):e2726. PMID: 28383547
BIX-01294 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2017 Apr 6;8(4):e2726. [Abstract]
Upper and middle panels: WB detection of H3K9me2 and G9A in PC9 and A549 cells treated with 0, 1, 2.5, 5 or 10 μM BIX-01294. The total level of histone H3 and actin serve as loading controls. Lower panel: WB detection of the total (T-) level of and phosphorylated (P-) ERK kinase in PC9 and A549 cells treated with 0, 1, 5 or 10 μM BIX-01294. GAPDH serves as the loading control
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Cell Syst
A Library of Phosphoproteomic and Chromatin Signatures for Characterizing Cellular Responses to Drug Perturbations. [Abstract]2018 Apr 25;6(4):424-443.e7. PMID: 29655704 -
NPJ Regen Med
Epigenetic mechanisms regulate sex differences in cardiac reparative functions of bone marrow progenitor cells. [Abstract]2024 Apr 29;9(1):17. PMID: 38684697
BIX-01294 purchased from MedChemExpress. Usage Cited in: NPJ Regen Med. 2024 Apr 29;9(1):17. [Abstract]
CCL3 was found to be the most altered cytokine by inhibiting G9a using BIX-01294 (1 μM). Inhibition of G9a using BIX-01294 (1 μM) largely altered the secretion in the F-EPC and OVX-EPC. ELISA showed secretory upregulation of CCL3 and VEGF-A in M-EPC, F-EPC, and OVX-EPC with the inhibition of G9a using BIX-01294.
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Biomolecules
Targeting G9a Exerts Pleiotropic Suppression in Triple-Negative Breast Cancer Cells: Cooperatively Inducing Pyroptosis and Apoptosis. [Abstract]2026 Feb 25;16(3):345. PMID: 41897283 -
Molecules
Modulatory Effect of Curcumin on Expression of Methyltransferase/Demethylase in Colon Cancer Cells: Impact on wt p53, mutp53 and c-Myc. [Abstract]2025 Jul 22;30(15):3054. PMID: 40807229 -
J Cell Mol Med
Larotrectinib induces autophagic cell death through AMPK/mTOR signalling in colon cancer. [Abstract]2022 Nov;26(21):5539-5550. PMID: 36251949 -
iScience
miR-1307 promotes hepatocarcinogenesis by CALR-OSTC-endoplasmic reticulum protein folding pathway. [Abstract]2021 Oct 14;24(11):103271. PMID: 34761190 -
Aging (Albany NY)
Uhrf1 regulates H3K9me2 modification of mTOR to inhibit the effect of autophagy in myocardial ischemia-reperfusion injury. [Abstract]2021 Mar 19;13(7):9704-9718. PMID: 33744855 -
Biomedicines
Exosome-Mediated Differentiation of Mouse Embryonic Fibroblasts and Exocrine Cells into β-Like Cells and the Identification of Key miRNAs for Differentiation. [Abstract]2020 Nov 9;8(11):485. PMID: 33182285 -
Biochim Biophys Acta
2018 May;1864(5 Pt A):1744-1753. PMID: 29499325 -
Mol Med Rep
G9a inhibition promotes the formation of pacemaker-like cells by reducing the enrichment of H3K9me2 in the HCN4 promoter region. [Abstract]2023 Feb;27(2):21. PMID: 36484369 -
Mol Med Rep
H3K9me2 regulates early transcription factors to promote mesenchymal stem‑cell differentiation into cardiomyocytes. [Abstract]2021 Aug;24(2):616. PMID: 34184085 -
Exp Cell Res
Lamin B2 promotes the malignant phenotype of non-small cell lung cancer cells by upregulating dimethylation of histone 3 lysine 9. [Abstract]2020 Aug 15;393(2):112090. PMID: 32416090 -
Invest New Drugs
BIX-01294, a G9a inhibitor, suppresses cell proliferation by inhibiting autophagic flux in nasopharyngeal carcinoma cells. [Abstract]2021 Jun;39(3):686-696. PMID: 33387131 -
PLoS One
DNA methylation cooperates with H3K9me2 at HCN4 promoter to regulate the differentiation of bone marrow mesenchymal stem cells into pacemaker-like cells. [Abstract]2023 Aug 29;18(8):e0289510. PMID: 37643180 -
Biochem Biophys Res Commun
Inhibition of histone-lysine N-methyltransferase G9a exacerbates alcoholic steatohepatitis (ASH) in mice. [Abstract]2025 Nov 29:794:153072. PMID: 41344213 -
Biochem Biophys Res Commun
Inhibition of histone methyltransferase G9a aggravates phenotypic severity of hepatic lipotoxicity in non-alcoholic steatohepatitis (NASH). [Abstract]2025 Jan:743:151171. PMID: 39693938 -
Cell J
2023 Feb 1;25(2):118-125. PMID: 36840458 -
bioRxiv
A host-directed adjuvant resuscitates and sensitizes intracellular bacterial persisters to antibiotics. [Abstract]2024 Oct 30:2024.09.30.615828. PMID: 39554024 -
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Solvent & Solubility
DMSO : ≥ 110 mg/mL (224.20 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.75 mg/mL (5.60 mM); Clear solution
This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.75 mg/mL (5.60 mM); Clear solution
This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (283 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Nathaniel W Mabe, et al. G9a Promotes Breast Cancer Recurrence through Repression of a Pro-inflammatory Program. Cell Rep. 2020 Nov 3;33(5):108341. [Content Brief]
[2]. Kubicek S, et al. Reversal of H3K9me2 by a small-molecule inhibitor for the G9a histone methyltransferase. Mol Cell. 2007 Feb 9;25(3):473-81. [Content Brief]
[3]. Yang Q, et al. BIX-01294 treatment blocks cell proliferation, migration and contractility in ovine foetal pulmonary arterial smooth muscle cells. Cell Prolif. 2012 Aug;45(4):335-44. [Content Brief]
[4]. Iwona Anna Ciechomska, et al. BIX01294, an inhibitor of histone methyltransferase, induces autophagy-dependent differentiation of glioma stem-like cells. Sci Rep [Content Brief]
[5]. Yanqi Chang, et al.Structural basis for G9a-like protein lysine methyltransferase inhibition by BIX-01294. Nat Struct Mol Biol. 2009 Mar;16(3):312-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0382 mL | 10.1908 mL | 20.3815 mL | 50.9539 mL |
| 5 mM | 0.4076 mL | 2.0382 mL | 4.0763 mL | 10.1908 mL | |
| 10 mM | 0.2038 mL | 1.0191 mL | 2.0382 mL | 5.0954 mL | |
| 15 mM | 0.1359 mL | 0.6794 mL | 1.3588 mL | 3.3969 mL | |
| 20 mM | 0.1019 mL | 0.5095 mL | 1.0191 mL | 2.5477 mL | |
| 25 mM | 0.0815 mL | 0.4076 mL | 0.8153 mL | 2.0382 mL | |
| 30 mM | 0.0679 mL | 0.3397 mL | 0.6794 mL | 1.6985 mL | |
| 40 mM | 0.0510 mL | 0.2548 mL | 0.5095 mL | 1.2738 mL | |
| 50 mM | 0.0408 mL | 0.2038 mL | 0.4076 mL | 1.0191 mL | |
| 60 mM | 0.0340 mL | 0.1698 mL | 0.3397 mL | 0.8492 mL | |
| 80 mM | 0.0255 mL | 0.1274 mL | 0.2548 mL | 0.6369 mL | |
| 100 mM | 0.0204 mL | 0.1019 mL | 0.2038 mL | 0.5095 mL |