2. Metabolic Enzyme/Protease
  3. Cytochrome P450
  4. Exemestane

Exemestane (FCE 24304) is a selective, irreversible and orally active steroidal aromatase inhibitor with IC50s of 30 nM and 40 nM for human placental and rat ovarian aromatase, respectively. Exemestane can be used for hormone-dependent breast cancer research.

For research use only. We do not sell to patients.

CAS No. : 107868-30-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of Exemestane:

Exemestane Related Antibodies

Top Publications Citing Use of Products

    Exemestane purchased from MedChemExpress. Usage Cited in: Mol Oncol. 2025 Jul 13.  [Abstract]

    Cell viability of MDA‐MB‐231 TNBC, MDA‐MB‐436 TNBC, HCC1937 TNBC, MCF7 ER‐positive breast cancer, and MCF10A normal breast cells following 24 h treatment with concentration gradients of Exemestane, with and without 10 μm Olaparib, as determined by MTT assay.

    Exemestane purchased from MedChemExpress. Usage Cited in: Mol Oncol. 2025 Jul 13.  [Abstract]

    Clonogenic survival assay of MDA‐MB‐231, MDA‐MB‐436 cells treated with Exemestane (EXE, 25 μm) and Olaparib (OLAP, 10 μm) alone or in combination (24 h treatment).

    Exemestane purchased from MedChemExpress. Usage Cited in: Mol Oncol. 2025 Jul 13.  [Abstract]

    Cell cycle distribution of MDA‐MB‐231 cells treated with Exemestane (25 μm) and Olaparib (10 μm) alone or in combination, 72 h treatment, as determined by PI staining and flow cytometry.

    Exemestane purchased from MedChemExpress. Usage Cited in: Mol Oncol. 2025 Jul 13.  [Abstract]

    Western blot analysis of DDR activation following Exemestane treatment (25, 50 and 100 μm) for 3 h.

    Exemestane purchased from MedChemExpress. Usage Cited in: Mol Oncol. 2025 Jul 13.  [Abstract]

    RPA1 foci (green) formation in MDA‐MB‐231 cells nuclei following treatment with Exemestane (50 μm, 24 h). Cisplatin (CIS, 50 μm, 24 h) was employed as a positive control. Nuclear staining by DAPI (blue) also included.

    View All Cytochrome P450 Isoform Specific Products:

    View All Isoforms
    CYP1 CYP2 CYP3 CYP4 CYP11 CYP17 Aromatase/CYP19A1 CYP26 CYP51 CYP1A2 CYP2D6 CYP2C9 CYP2C19 CYP3A CYP3A4 CYP17A1

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Exemestane (FCE 24304) is a selective, irreversible and orally active steroidal aromatase inhibitor with IC50s of 30 nM and 40 nM for human placental and rat ovarian aromatase, respectively. Exemestane can be used for hormone-dependent breast cancer research[1][2].

    IC50 & Target

    Aromatase

     

    Cellular Effect
    Cell Line Type Value Description References
    MCF7 IC50
    0.9 μM
    Compound: 1, Exemestane
    Inhibition of human placental microsome aromatase expressed in human MCF7 cells using [1beta-3H] androstenedione as substrate after 1 hr by liquid scintillation counting
    Inhibition of human placental microsome aromatase expressed in human MCF7 cells using [1beta-3H] androstenedione as substrate after 1 hr by liquid scintillation counting
    		[PMID: 25277066]
    In Vitro

    Exemestane (EXE; 1-1000 nM; 72 hours; hFOB, Saos-2 cells<) treatment significantly increases the number of the cells[2].
    ? Exemestane (72 hours) increases alkaline phosphatase activity in hFOB and Saos-2 cells and induces the expression of MYBL2, OSTM1, HOXD11, ADCYAP1R1, and glypican 2 in hFOB cells[2].
    ? Exemestane competitively inhibits and time-dependently inactivates of human placental aromatase with Ki of 4.3 nM. Exemestane displaces [3H]5α-dihydrotestosterone from rat prostate androgen receptor with IC50 of 0.9 μM[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Exemestane Related Antibodies

    Cell Viability Assay[2]

    Cell Line: hFOB, Saos-2 cells
    Concentration: 1 nM, 10 nM, 100 nM, 1000 nM
    Incubation Time: 72 hours
    Result: Induced cell proliferation.
    In Vivo

    Exemestane (EXE; 20-100 mg/kg; intramuscular injection; once weekly; for 16 weeks) treatment significantly increases the lumbar vertebral and femoral BMD, bending strength of the femur, compressive strength of the fifth lumbar vertebra, and trabecular bone volume. Exemestane significantly reduces an ovariectomy-induced increase in serum pyridinoline and serum osteocalcin. Exemestane causes significant reductions of serum cholesterol and low-density lipoprotein cholesterol[3].
    ? Exemestane (20 mg/kg/day s.c.) induces 26% complete (CR) and 18% partial (PR) tumor regressions in rats with 7,12-dimethylbenzanthracene (DMBA)-induced mammary tumors[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Female Sprague Dawley rats (10-month-old) bearing ovariectomy [3]
    Dosage: 20 mg/kg, 50 mg/kg, or 100 mg/kg
    Administration: Intramuscular injection; once weekly; for 16 weeks
    Result: Significantly increased the lumbar vertebral and femoral BMD, bending strength of the femur, compressive strength of the fifth lumbar vertebra, and trabecular bone volume.
    Clinical Trial
    Molecular Weight

    296.40

    Formula

    C20H24O2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C[C@]1([C@](CC2)([H])[C@]3([H])CC(C4=CC(C=C[C@]4(C)[C@@]3([H])CC1)=O)=C)C2=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 54 mg/mL (182.19 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.3738 mL 16.8691 mL 33.7382 mL
    5 mM 0.6748 mL 3.3738 mL 6.7476 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (8.43 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (8.43 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    (per animal)

    g

    Dosing volume
    (per animal)

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.97%

    SDS (418 KB)

    COA (253 KB) HNMR (277 KB) RP-HPLC (243 KB) MS (69 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.3738 mL 16.8691 mL 33.7382 mL 84.3455 mL
    5 mM 0.6748 mL 3.3738 mL 6.7476 mL 16.8691 mL
    10 mM 0.3374 mL 1.6869 mL 3.3738 mL 8.4345 mL
    15 mM 0.2249 mL 1.1246 mL 2.2492 mL 5.6230 mL
    20 mM 0.1687 mL 0.8435 mL 1.6869 mL 4.2173 mL
    25 mM 0.1350 mL 0.6748 mL 1.3495 mL 3.3738 mL
    30 mM 0.1125 mL 0.5623 mL 1.1246 mL 2.8115 mL
    40 mM 0.0843 mL 0.4217 mL 0.8435 mL 2.1086 mL
    50 mM 0.0675 mL 0.3374 mL 0.6748 mL 1.6869 mL
    60 mM 0.0562 mL 0.2812 mL 0.5623 mL 1.4058 mL
    80 mM 0.0422 mL 0.2109 mL 0.4217 mL 1.0543 mL
    100 mM 0.0337 mL 0.1687 mL 0.3374 mL 0.8435 mL
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    Exemestane Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Exemestane107868-30-4FCE 24304 EXEFCE24304FCE-24304Cytochrome P450CYPsSteroidalaromataseandrostenedionealkalinephosphatase5α-dihydrotestosteroneInhibitorinhibitorinhibit

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