MS023

Name: MS023
Brand: MedChemExpress
SKU: HY-19615
Rating: 5.0 (22 reviews)

Cat. No.: HY-19615 Purity: 99.93%: Data Sheet
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SDS
Handling Instructions
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MS023, a chemical probe, is a potent, selective, and cell-active inhibitor of human type I protein arginine methyltransferases (PRMTs) inhibitor, with IC₅₀s of 30, 119, 83, 4 and 5 nM for PRMT1, PRMT3, PRMT4, PRMT6, and PRMT8, respectively.

For research use only. We do not sell to patients.

CAS No. : 1831110-54-3

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
2 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
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Customer Review

Based on 22 publication(s) in Google Scholar

Other Forms of MS023:

MS023 dihydrochloride In-stock
MS023 trihydrochloride Get quote

22 Publications Citing Use of MCE MS023

Cell Imaging/Staining

Cell Proliferation/Viability Assay

RT-PCR

: MS023 purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2026 Jan 20;7(1):102542. [Abstract]; The drug response curve and IC50 value for PRMT inhibitor MS023 and GSK591 combinations after U2AF2 knockdown in THP1 leukemia cells. Cell viability was assessed using a CCK8 assay.

: MS023 purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2026 Jan 20;7(1):102542. [Abstract]; RT-PCR results detecting aberrantly spliced transcripts in THP1 leukemia cells, which were treated with PRMT inhibitor MS023 or GSK591 with or without U2AF2 knockdown.

: MS023 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 22;16(1):949. [Abstract]; Representative images of FRAP of MSX1-mEGFP- expressing HEK293T treated with DMSO or MS023.

: MS023 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jan 22;16(1):949. [Abstract]; Representative western blot images of Co-IP assays assessing the aDMA of MSX1 in treated with MS023 or PRMT1 siRNA.

: MS023 purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Mar 8;23(1):126. [Abstract]; Immunoprecipitation and western blot analysis of arginine dimethylation in METTL14 overexpressed in HEK293T cells. Cells were treated with 1 μM EPZ015666, 1 μM MS023, or DMSO for 72 h and METTL14 is immunoprecipitated by anti-METTL14 antibody.

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View All Histone Methyltransferase Isoform Specific Products:

View All Isoforms

EZH1 EZH2 DOT1L SMYD2 SMYD3 SUV39H2/KMT1B EHMT2/G9a/KMT1C EHMT1/GLP/KMT1D ASH1L/KMT2H SETDB1/KMT2G SETD2/KMT3A NSD2/MMSET/WHSC1 NSD3/WHSC1L1 SETD7/KMT7 SETD8/KMT5A PRMT1 PRMT3 PRMT4 PRMT5 PRMT6 PRMT7 PRMT8

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

PRMT1

PRMT3

PRMT6

PRMT8

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	13.4 μM Compound: Chemical probe: MS023	Antiproliferative against human A549 cells expressing MTAP clone 2 assessed as reduction of cell death treated for 7 days with replenishment of medium for every 48 hrs by MTT assay (Rvb = 4.4 uM) Antiproliferative against human A549 cells expressing MTAP clone 2 assessed as reduction of cell death treated for 7 days with replenishment of medium for every 48 hrs by MTT assay (Rvb = 4.4 uM)	[PMID: 33691794]
A549	IC₅₀	5.1 μM Compound: Chemical probe: MS023	Antiproliferative against human A549 cells expressing MTAP clone 1 assessed as reduction of cell death treated for 7 days with replenishment of medium for every 48 hrs by MTT assay (Rvb = 4.4 uM) Antiproliferative against human A549 cells expressing MTAP clone 1 assessed as reduction of cell death treated for 7 days with replenishment of medium for every 48 hrs by MTT assay (Rvb = 4.4 uM)	[PMID: 33691794]
HCC4006	IC₅₀	0.47 μM Compound: Chemical probe: MS023	Antiproliferative against human HCC4006 cells expressing MTAP clone 1 assessed as reduction of cell death treated for 7 days with replenishment of medium for every 48 hrs by MTT assay (Rvb = 0.24 uM) Antiproliferative against human HCC4006 cells expressing MTAP clone 1 assessed as reduction of cell death treated for 7 days with replenishment of medium for every 48 hrs by MTT assay (Rvb = 0.24 uM)	[PMID: 33691794]
HCC4006	IC₅₀	0.56 μM Compound: Chemical probe: MS023	Antiproliferative against human HCC4006 cells expressing MTAP clone 2 assessed as reduction of cell death treated for 7 days with replenishment of medium for every 48 hrs by MTT assay (Rvb = 0.24 uM) Antiproliferative against human HCC4006 cells expressing MTAP clone 2 assessed as reduction of cell death treated for 7 days with replenishment of medium for every 48 hrs by MTT assay (Rvb = 0.24 uM)	[PMID: 33691794]
SK-LU-1	IC₅₀	36.7 μM Compound: Chemical probe: MS023	Antiproliferative against human SK-LU-1 cells expressing MTAP clone 1 assessed as reduction of cell death treated for 7 days with replenishment of medium for every 48 hrs by MTT assay (Rvb = 16.3 uM) Antiproliferative against human SK-LU-1 cells expressing MTAP clone 1 assessed as reduction of cell death treated for 7 days with replenishment of medium for every 48 hrs by MTT assay (Rvb = 16.3 uM)	[PMID: 33691794]
SK-LU-1	IC₅₀	41 μM Compound: Chemical probe: MS023	Antiproliferative against human SK-LU-1 cells expressing MTAP clone 2 assessed as reduction of cell death treated for 7 days with replenishment of medium for every 48 hrs by MTT assay (Rvb = 16.3 uM) Antiproliferative against human SK-LU-1 cells expressing MTAP clone 2 assessed as reduction of cell death treated for 7 days with replenishment of medium for every 48 hrs by MTT assay (Rvb = 16.3 uM)	[PMID: 33691794]

In Vitro

MS023 (1-1000 nM; 48 hours) inhibits PRMT1 methyltransferase activity in MCF7 cells^[1].
MS023(1-1000 nM; 20 hours) inhibits PRMT6 methyltransferase activity in HEK293 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	MCF7 and HEK293 cells
Concentration:	1.4, 4, 12, 37, 111, 333, and 1000 nM
Incubation Time:	48 hours for MCF7 cells; 20 hours for HEK293 cells
Result:	Treatment potently and concentration-dependently reduced cellular levels of H4R3me2a (IC₅₀=9±0.2 nM). Treatment concentration-dependently reduced the H3R2me2a mark (IC₅₀=56±7 nM).

In Vivo

Administration of MS023 (160 mg/kg, i.p) in combination with PKC412 (100 mg/kg, i.g.) blocks MLL-r acute lymphoblastic leukemia (ALL) propagation by inhibiting maintenance of functional MLL-r ALL-initiating cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	NOD-scid IL2Rgnull (NSG) mice bearing primary MLL-r ALL cells^[2]
Dosage:	160 mg/kg
Administration:	Intraperitoneal injection; PKC412 (100 mg/kg, i.g.), MS023 (160 mg/kg, i.p), or a combination for 4 weeks
Result:	Combinatorial treatment extended survival of leukemic mice relative to single treatments.

Molecular Weight

287.40

Formula

C₁₇H₂₅N₃O

CAS No.

1831110-54-3

Appearance

Solid

Color

White to light yellow

SMILES

CN(CCN)CC1=CNC=C1C2=CC=C(OC(C)C)C=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (347.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.4795 mL	17.3974 mL	34.7947 mL
5 mM	0.6959 mL	3.4795 mL	6.9589 mL
10 mM	0.3479 mL	1.7397 mL	3.4795 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: Saline
Solubility: 53.33 mg/mL (185.56 mM); Clear solution; Need ultrasonic and adjust pH to 8 with 1M HCl

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.93%

Select Batch:

Data Sheet (275 KB) SDS (396 KB)

COA (248 KB) HNMR (241 KB) RP-HPLC (244 KB) MS (205 KB)

Handling Instructions (2659 KB)

References

[1]. Eram MS, et al. A Potent, Selective, and Cell-Active Inhibitor of Human Type I Protein Arginine Methyltransferases. ACS Chem Biol. 2016 Mar 18;11(3):772-81. [Content Brief]

[2]. Yinghui Zhu, et al. Targeting PRMT1-mediated FLT3 methylation disrupts maintenance of MLL-rearranged acute lymphoblastic leukemia. Blood. 2019 Oct 10;134(15):1257-1268. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.4795 mL	17.3974 mL	34.7947 mL	86.9868 mL
	5 mM	0.6959 mL	3.4795 mL	6.9589 mL	17.3974 mL
	10 mM	0.3479 mL	1.7397 mL	3.4795 mL	8.6987 mL
	15 mM	0.2320 mL	1.1598 mL	2.3196 mL	5.7991 mL
	20 mM	0.1740 mL	0.8699 mL	1.7397 mL	4.3493 mL
	25 mM	0.1392 mL	0.6959 mL	1.3918 mL	3.4795 mL
	30 mM	0.1160 mL	0.5799 mL	1.1598 mL	2.8996 mL
	40 mM	0.0870 mL	0.4349 mL	0.8699 mL	2.1747 mL
	50 mM	0.0696 mL	0.3479 mL	0.6959 mL	1.7397 mL
	60 mM	0.0580 mL	0.2900 mL	0.5799 mL	1.4498 mL
	80 mM	0.0435 mL	0.2175 mL	0.4349 mL	1.0873 mL
	100 mM	0.0348 mL	0.1740 mL	0.3479 mL	0.8699 mL

MS023 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MS0231831110-54-3MS 023MS-023Histone MethyltransferaseInhibitorinhibitorinhibit

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MS023

Customer Review

Other Forms of MS023:

MS023 Related Antibodies

22 Publications Citing Use of MCE MS023

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•Related Screening Libraries:

•Related Small Molecules:

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View All Histone Methyltransferase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

MS023 Related Antibodies

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Complete Stock Solution Preparation Table

MS023 Related Classifications

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