Cat. No.: HY-N2041

Myristic acid

7 Cited Publications

Customer Review

Based on 7 publication(s) in Google Scholar

Myristic acid is an orally active saturated 14-carbon fatty acid found in most animal and plant fats, especially milk fat coconut oil, palm oil and nutmeg oil. Myristic acid exerts anti-inflammatory activity through the NF-κB pathway. Myristic acid has antibacterial, anti-inflammatory and analgesic properties.

For research use only. We do not sell to patients.

Purity: 99.88%
CAS No.: 544-63-8
Formula: C₁₄H₂₈O₂
Molecular Weight:228.37

Storage:

Store at room temperature 3 years.

In solvent -80°C, 2 years , -20°C, 1 year

Myristic acid Chemical Structure

CAS No.: 544-63-8

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (7)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Human Endogenous Metabolite

All NF-κB Isoforms :

All Endogenous Metabolite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	2 x 10² μM Compound: MA	Cytotoxicity against human A549 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay Cytotoxicity against human A549 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay	[PMID: 37462318]
Caco-2	IC₅₀	>1 x 10³ μM Compound: MA	Cytotoxicity against human Caco-2 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay Cytotoxicity against human Caco-2 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay	[PMID: 37462318]
CHO	EC₅₀	11.4 μM Compound: 20	Agonist activity at recombinant human GPR84 expressed in CHO cell membranes co-expressing beta-arrestin2 assessed as inhibition of forskolin-stimulated [3H]cAMP accumulation preincubated for 5 mins followed by forskolin stimulation and measured after 15 m Agonist activity at recombinant human GPR84 expressed in CHO cell membranes co-expressing beta-arrestin2 assessed as inhibition of forskolin-stimulated [3H]cAMP accumulation preincubated for 5 mins followed by forskolin stimulation and measured after 15 m	[PMID: 31721581]
CHO	EC₅₀	7.01 μM Compound: 20	Agonist activity at recombinant human GPR84 expressed in CHO cell membranes co-expressing beta-arrestin2 assessed as beta-arrestin 2 recruitment measured after 90 mins by beta-galactosidase based PathHunter assay Agonist activity at recombinant human GPR84 expressed in CHO cell membranes co-expressing beta-arrestin2 assessed as beta-arrestin 2 recruitment measured after 90 mins by beta-galactosidase based PathHunter assay	[PMID: 31721581]
Huh-7	CC₅₀	>50 μM Compound: Myristic acid	Cytotoxicity against human Huh-7 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo analysis Cytotoxicity against human Huh-7 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo analysis	[PMID: 34583312]
THP-1	IC₅₀	5 μM Compound: myristic acid	Inhibition of TLR2 agonist Pam3Cys-Ser-(Lys)4-OH-mediated IL8 secretion in THP1 cells Inhibition of TLR2 agonist Pam3Cys-Ser-(Lys)4-OH-mediated IL8 secretion in THP1 cells	[PMID: 16509590]

In Vitro

Myristic acid (100, 150, 200 μM, 24 h) regulates the production of triglyceride in bovine mammary epithelial cells through ubiquitination pathway^[1].
Myristic acid (3-1000 μM, 10 min) can inhibit the activity of bacterial ABC transporter BmrA^[2].
Myristic acid (12.5-200 μg/mL, 24 h) exerts in vitro anti-inflammatory activity by increasing (58%) IL-10 production in LPS (HY-D1056)-stimulated macrophages^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

MAC-T
Concentration:

100, 150, 200 μM
Incubation Time:

24 h
Result:

Increased the level of protein ubiquitination.

Cell Viability Assay^[3]

Cell Line:

J774A.1 macrophages
Concentration:

12.5, 25, 50, 100,200 μg/mL
Incubation Time:

24 h
Result:

Showed non-cytotoxic effects in LPS-stimulated J774A.1 macrophages at 25 μg/mL.

In Vivo

Myristic acid (12.5-100 mg/kg, orally, Single dose) has good anti-inflammatory effect on ear edema induced by acute (ED50 = 62 mg/kg) and chronic (ED50 = 77 mg/kg) mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

TPA-induced ear edema in mice^[3]
Dosage:

12.5, 25, 50, 100 mg/kg
Administration:

p.o.
Result:

Decreased ear edema inflammation in the acute and chronic TPA assays with IC₅₀ values of 62 and 77 mg/kg, respectively in a dose-dependent manner.
Attenuated the acetic acid-induced abdominal contortions with ED₅₀ values of 32 mg/kg.

Chemical Information

CAS No. 544-63-8
Appearance Solid
Molecular Weight 228.37
Formula C₁₄H₂₈O₂
Color White to off-white
SMILES

CCCCCCCCCCCCCC(O)=O
Structure Classification
- Ketones, Aldehydes, Acids
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Publications (7)

All Bio/Physico-chemical AssayCell Proliferation/Viability AssayIn Vivo Efficacy Study

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Journal Impact Factor

Nature

Lithocholic acid phenocopies anti-ageing effects of calorie restriction. [Abstract]

2025 Jul;643(8070):192-200. PMID: 39695227
Redox Biol

Niraparib restricts intraperitoneal metastases of ovarian cancer by eliciting CD36-dependent ferroptosis. [Abstract]

2025 Feb 3:80:103528. PMID: 39922130

Myristic acid purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Feb 3:80:103528. [Abstract]

OVCAR8 and A2780 cells were cultured in fatty acid-free media supplemented with 50 μM AA (arachidonic acid), 50 μM OA (oleic acid), 50 μM EPA (eicosapentaenoic acid), 50 μM PA (Palmitic acid), and 50 μM MA (myristic acid) with the treatment of 10 μM niraparib for 72 h, and the cell viability was determined.
Cell Rep Med

High-fat diet increases circulating palmitic acid produced by gut Bacteroides thetaiotaomicron to promote thrombosis. [Abstract]

2025 Aug 19;6(8):102260. PMID: 40749683

Myristic acid purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Aug 19;6(8):102260. [Abstract]

Myristic acid (20 μg/mL; 4-6 h). PA inhibited the amidolysis of APC on the chromogen substrate S-2366, whereas POA, MA, OA, and EA had no effect on APC activity (n = 3).

Myristic acid purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Aug 19;6(8):102260. [Abstract]

Intravenous administration of PA promoted venous thrombosis in the model of venous thrombosis induced by ligating the inferior vena cava, whereas POA, MA, OA, and EA had no effect on venous thrombosis. The inferior vena cava was removed 24 h later for H&E staining to observe thrombosis (left), and the weight of venous thrombus in each group was counted (right) (n = 6).

Myristic acid purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Aug 19;6(8):102260. [Abstract]

Intravenous administration of PA promoted arterial thrombosis in the model of carotid artery thrombosis induced by 10% FeCl₃, whereas POA, MA, OA, and EA had no effect on arterial thrombosis. Representative images of carotid artery blood flow (top) by laser speckle perfusion imaging were shown, and the region of interest (orange rectangle) was placed in the carotid artery to quantify blood flow change. Blood flow change in the region of interest was shown (bottom) by using perfusion units (n = 6).

Myristic acid purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Aug 19;6(8):102260. [Abstract]

PA promoted thrombin generation as evaluated by thrombin generation assays (TGA) in human plasma, including the thrombin generation curve.
Cell Rep

STBD1 mediates the crosstalk between glycogen and lipid droplets in clear cell renal cell carcinoma. [Abstract]

2025 Oct 15;44(10):116429. PMID: 41105508
Commun Biol

Structural characteristics of alpha-fetoprotein, including N-glycosylation, metal ion and fatty acid binding sites. [Abstract]

2024 Apr 27;7(1):505. PMID: 38678117
Front Cell Dev Biol

Licarin-B Exhibits Activity Against the Toxoplasma gondii RH Strain by Damaging Mitochondria and Activating Autophagy. [Abstract]

2021 Jun 11:9:684393. PMID: 34179016
Eur J Lipid Sci Technol

2023 Feb 25.

Solvent & Solubility

In Vitro:

DMSO : 200 mg/mL (875.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 100 mg/mL (437.89 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.3789 mL

21.8943 mL

43.7886 mL

5 mM

0.8758 mL

4.3789 mL

8.7577 mL

10 mM

0.4379 mL

2.1894 mL

4.3789 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 5 mg/mL (21.89 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 5 mg/mL (21.89 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Protocol 3

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (10.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: Cremophor EL

Solubility: 40 mg/mL (175.15 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Hu M, et al. Myristic acid regulates triglyceride production in bovine mammary epithelial cells through the ubiquitination pathway. Agriculture, 2023, 13(10): 1870.

[2]. Oepen K, et al. Myristic Acid Inhibits the Activity of the Bacterial ABC Transporter BmrA. Int J Mol Sci. 2021 Dec 17;22(24):13565. [Content Brief]

[3]. Alonso-Castro AJ, et al. Myristic acid reduces skin inflammation and nociception. J Food Biochem. 2022 Jan;46(1):e14013. [Content Brief]

[4]. Huang Q, et al. Anti-inflammatory effects of myristic acid mediated by the NF-κB pathway in lipopolysaccharide-induced BV-2 microglial cells. Mol Omics. 2023 Oct 30;19(9):726-734. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
Ethanol / DMSO	1 mM	4.3789 mL	21.8943 mL	43.7886 mL	109.4715 mL
	5 mM	0.8758 mL	4.3789 mL	8.7577 mL	21.8943 mL
	10 mM	0.4379 mL	2.1894 mL	4.3789 mL	10.9471 mL
	15 mM	0.2919 mL	1.4596 mL	2.9192 mL	7.2981 mL
	20 mM	0.2189 mL	1.0947 mL	2.1894 mL	5.4736 mL
	25 mM	0.1752 mL	0.8758 mL	1.7515 mL	4.3789 mL
	30 mM	0.1460 mL	0.7298 mL	1.4596 mL	3.6490 mL
	40 mM	0.1095 mL	0.5474 mL	1.0947 mL	2.7368 mL
	50 mM	0.0876 mL	0.4379 mL	0.8758 mL	2.1894 mL
	60 mM	0.0730 mL	0.3649 mL	0.7298 mL	1.8245 mL
	80 mM	0.0547 mL	0.2737 mL	0.5474 mL	1.3684 mL
	100 mM	0.0438 mL	0.2189 mL	0.4379 mL	1.0947 mL

Keywords

Myristic acid

7 Cited Publications

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Myristic acid

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Publications Citing Use of MedChemExpress (MCE) Myristic acid

All NF-κB Isoforms

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (7)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Myristic acid Related Classifications

HY-N2041 Related Classifications