α-Linolenic acid (Synonyms: ALA free base; C18:3 (9Z,12Z,15Z) free base; C18:3 n-3 free base)

Name: α-Linolenic acid
Brand: MedChemExpress
SKU: HY-N0728
Rating: 5.0 (11 reviews)

Cat. No.: HY-N0728 Purity: 99.92%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

α-Linolenic acid (ALA (free base); C18:3 (9Z,12Z,15Z) (free base); C18:3 n-3 (free base)) is an essential fatty acid that cannot be synthesized by humans. α-Linolenic acid can affect the process of thrombotic through the modulation of PI3K/Akt signaling. α-Linolenic acid possess the anti-arrhythmic properties and is related to cardiovascular disease and cancer.

For research use only. We do not sell to patients.

CAS No. : 463-40-1

Size	Stock	Quantity
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	Get quote
5 g	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 11 publication(s) in Google Scholar

Other Forms of α-Linolenic acid:

11 Publications Citing Use of MCE α-Linolenic acid

Histological Imaging/Staining

Cell Proliferation/Viability Assay

In Vivo Efficacy Study

Bio/Physico-chemical Assay

RT-PCR

: α-Linolenic acid purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Nov:147:157214. [Abstract]; α-Linolenic acid (ALA, 500 mg/kg; Oral gavage; Once daily for 8 weeks) treatment effectively reduced weight gain starting at week 4.

: α-Linolenic acid purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Nov:147:157214. [Abstract]; α-Linolenic acid (ALA, 500 mg/kg; Oral gavage; Once daily for 8 weeks) significantly inhibited the increase of FBG in mice starting from week 6.

: α-Linolenic acid purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Nov:147:157214. [Abstract]; The mRNA expression levels of inflammatory cytokines (TNF-α, IL-6, IL-17A, IFN-γ, IL-10, IL-1β and IL-18) in the liver of db/db mice were quantified by qRT-PCR. The results showed that, compared with the CO group, a significant decrease in liver TNF-α, IL-6, IL-17A, interferon-γ (IFN-γ), IL-1β, and IL-18 levels was observed in theα-Linolenic acid (ALA, 500 mg/kg; Oral gavage; Once daily for 8 weeks)-treated group.

: α-Linolenic acid purchased from MedChemExpress. Usage Cited in: J Adv Res. 2024 May 7:S2090-1232(24)00183-8. [Abstract]; VIMTIN / K14, CD31 and FLT4 immunostaining shows angiogenesis after ALA (200 μM; s.c.) treatment in young mice; Statistics of CD31+ cells and FLT4+ cells. Scale bars, 50 μm.

: α-Linolenic acid purchased from MedChemExpress. Usage Cited in: Redox Biol. 2023 Oct:66:102857. [Abstract]; Percentage of dead FHs74Int cells, treated with indicated PUFAs (20 or 40 μM) or/and Fer-1 (5 μM), detected at 120 h after irradiation. αLA: α-Linolenic acid (40 μM); LA: Linoleic acid; DHA: Docosahexaenoic acid; AA: Arachidonic acid.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Akt Isoform Specific Products:

View All Isoforms

Akt Akt1 Akt2 Akt3

View All PI3K Isoform Specific Products:

View All Isoforms

PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

PI3K

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	2.6 μM Compound: 1, alpha-LA	Agonist activity at human GPR120-G-alpha-16 fusion protein expressed in Flp-in HEK293 cells assessed as effect on intracellular calcium concentration by FLIPR assay Agonist activity at human GPR120-G-alpha-16 fusion protein expressed in Flp-in HEK293 cells assessed as effect on intracellular calcium concentration by FLIPR assay	[PMID: 19007110]
HEK293	EC₅₀	5.2 μM Compound: 1, alpha-LA	Agonist activity at human GPR40 expressed in T-REx HEK293 cells assessed as effect on intracellular calcium concentration by FLIPR assay Agonist activity at human GPR40 expressed in T-REx HEK293 cells assessed as effect on intracellular calcium concentration by FLIPR assay	[PMID: 19007110]
HepG2	EC₅₀	0.002 μM Compound: Linolenic Acid	Activation of human PXR expressed in human HepG2 (DPX-2) cells assessed as induction of CYP3A4 after 24 hrs by luminescent analysis Activation of human PXR expressed in human HepG2 (DPX-2) cells assessed as induction of CYP3A4 after 24 hrs by luminescent analysis	[PMID: 20966043]
RBL-2H3	IC₅₀	> 50 μM Compound: 3	Antiallergic activity in rat RBL2H3 cells assessed as inhibition of DNP-HSA-mediated degranulation by measuring decrease in beta-hexosaminidase activity preincubated for 30 mins followed by DNP-HSA stimulation and measured after 30 mins by 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside substrate based assay Antiallergic activity in rat RBL2H3 cells assessed as inhibition of DNP-HSA-mediated degranulation by measuring decrease in beta-hexosaminidase activity preincubated for 30 mins followed by DNP-HSA stimulation and measured after 30 mins by 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside substrate based assay	[PMID: 31618024]

In Vitro

α-Linolenic acid converses into the longer chain fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

α-Linolenic acid (50, 100, 250 mg/kg; for 10 days) can completely inhibit collagen- and adrenaline-induced thrombosis in mice at 250 mg/kg^[1].
α-Linolenic acid (35, 70, 175 mg/kg) suppresses A-V thrombus formation in rats (weighing at 250 ~ 300 g)^[1].
α-Linolenic acid (70 or 175 mg/kg) inhibits collagen stimulated platelet aggregation in rats^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Mice
Dosage:	50, 100, 250 mg/kg
Administration:	i.v., 10 days
Result:	Inhibited collagen- and adrenaline-induced thrombosis in mice at 250 mg/kg.

Molecular Weight

278.43

Formula

C₁₈H₃₀O₂

CAS No.

463-40-1

Appearance

Liquid (Density: 0.914 g/cm³)

Color

Colorless to light yellow

SMILES

CC/C=C\C/C=C\C/C=C\CCCCCCCC(O)=O

Structure Classification

Others

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (359.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.5916 mL	17.9578 mL	35.9157 mL
5 mM	0.7183 mL	3.5916 mL	7.1831 mL
10 mM	0.3592 mL	1.7958 mL	3.5916 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 125 mg/mL (448.95 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (270 KB) SDS (393 KB)

COA (238 KB) HNMR (244 KB) RP-HPLC (215 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Yang Q, et al. Anti-thrombotic effects of α-linolenic acid isolated from Zanthoxylum bungeanum Maxim seeds. BMC Complement Altern Med. 2014 Sep 23;14:348. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.5916 mL	17.9578 mL	35.9157 mL	89.7892 mL
	5 mM	0.7183 mL	3.5916 mL	7.1831 mL	17.9578 mL
	10 mM	0.3592 mL	1.7958 mL	3.5916 mL	8.9789 mL
	15 mM	0.2394 mL	1.1972 mL	2.3944 mL	5.9859 mL
	20 mM	0.1796 mL	0.8979 mL	1.7958 mL	4.4895 mL
	25 mM	0.1437 mL	0.7183 mL	1.4366 mL	3.5916 mL
	30 mM	0.1197 mL	0.5986 mL	1.1972 mL	2.9930 mL
	40 mM	0.0898 mL	0.4489 mL	0.8979 mL	2.2447 mL
	50 mM	0.0718 mL	0.3592 mL	0.7183 mL	1.7958 mL
	60 mM	0.0599 mL	0.2993 mL	0.5986 mL	1.4965 mL
	80 mM	0.0449 mL	0.2245 mL	0.4489 mL	1.1224 mL
	100 mM	0.0359 mL	0.1796 mL	0.3592 mL	0.8979 mL

α-Linolenic acid Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

α-Linolenic acid (Synonyms: ALA free base; C18:3 (9Z,12Z,15Z) free base; C18:3 n-3 free base)

Customer Review

Other Forms of α-Linolenic acid:

α-Linolenic acid Related Antibodies

11 Publications Citing Use of MCE α-Linolenic acid

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Akt Isoform Specific Products:

View All PI3K Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

α-Linolenic acid Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

α-Linolenic acid Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report