1. JAK/STAT Signaling
    Stem Cell/Wnt
    Anti-infection
  2. STAT
    Bacterial
  3. Nifuroxazide

Nifuroxazide 

Cat. No.: HY-B1436 Purity: 98.51%
Handling Instructions

Nifuroxazide is an effective inhibitor of STAT3, also exerts potent anti-tumor and anti-metastasis activity.

For research use only. We do not sell to patients.

Nifuroxazide Chemical Structure

Nifuroxazide Chemical Structure

CAS No. : 965-52-6

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10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
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ready for reconstitution
USD 66 In-stock
Estimated Time of Arrival: December 31
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200 mg USD 60 In-stock
Estimated Time of Arrival: December 31
500 mg USD 96 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

Nifuroxazide is an effective inhibitor of STAT3, also exerts potent anti-tumor and anti-metastasis activity.

IC50 & Target

STAT3

 

In Vitro

When U266 cells are incubated with Nifuroxazide, a significant dose-dependent decrease in STAT3 tyrosine phosphorylation is observed. This inhibition of STAT3 tyrosine phosphorylation is rapid, occurring as early as 1 h after treatment, and is sustained for at least 24 h. Treatment of U266 or INA6 cells with Nifuroxazide for 48 hours result in a dose-dependent loss of cell viability with an EC50 of approximately 4.5 μM in both cell types. Notably, the MM cells lacking constitutive STAT3 activation show little toxicity to Nifuroxazide[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Compared with the vehicle group, treatment with Nifuroxazide could inhibit tumor growth and tumor weight in a dose-dependent manner, with the inhibition rate of tumor volumes being 43.0% and 62.1% at 25 mg/kg and 50 mg/kg, respectively. It is also shown that Nifuroxazide significantly inhibits the proliferation of nuclear Ki-67-positive cells and induces apoptosis cells of cleaved caspase-3-positive cells. Besides, it is found that treatment with Nifuroxazide could inhibit the expression of MMP-2, MMP-9 and p-Stat3 in A375 tumor tissues. What’s more, Nifuroxazide inhibits the infiltration of MDSCs into the lung, which might be associated with suppression of distant colonization of tumor cells in B16-F10 melanoma metastasis model[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

275.22

Formula

C₁₂H₉N₃O₅

CAS No.
SMILES

O=C(N/N=C/C1=CC=C([N+]([O-])=O)O1)C2=CC=C(O)C=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 155 mg/mL (563.19 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6335 mL 18.1673 mL 36.3346 mL
5 mM 0.7267 mL 3.6335 mL 7.2669 mL
10 mM 0.3633 mL 1.8167 mL 3.6335 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.58 mg/mL (9.37 mM); Suspended solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.58 mg/mL (9.37 mM); Suspended solution

*All of the co-solvents are provided by MCE.
References
Animal Administration
[2]

Mice[2]
Mice engrafted subcutaneously with 1×107 A375 cells are randomly divided into groups when tumor volume is around 100 mm3 and are administrated intraperitoneally injected with Nifuroxazide 25 mg/kg, 50 mg/kg or vehicle once daily. The tumor size and body weight are measured every 3 days. C57Bl/6J mice are engrafted by injecting intravenously via the tail vein with 2×105 B16-F10 cells to produce experimental lung metastasis. They are randomly assigned to groups on day 6 and are intraperitoneally injected with Nifuroxazide 50 mg/kg or vehicle once daily. Black dots on lung surface are counted and confirmed as melanoma metastases[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 98.51%

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Keywords:

NifuroxazideSTATBacterialInhibitorinhibitorinhibit

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