STF-31

Name: STF-31
Brand: MedChemExpress
SKU: HY-18728
Rating: 5.0 (17 reviews)

Cat. No.: HY-18728 Purity: 99.79%: Data Sheet
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STF-31 is a selective inhibitor of glucose transporter 1 (GLUT1), with an IC₅₀ of 1 μM. STF-31 is also a NAMPT inhibitor. STF-31 inhibits glucose uptake in renal cell carcinoma (RCC) 4 cells.

For research use only. We do not sell to patients.

CAS No. : 724741-75-7

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 17 publication(s) in Google Scholar

17 Publications Citing Use of MCE STF-31

RT-PCR

Flow Cytometry

: STF-31 purchased from MedChemExpress. Usage Cited in: Cell Biosci. 2024 Apr 16;14(1):48. [Abstract]; qRT-PCR assays evaluating the expression of proinflammatory phenotype markers, CD86, IL-6, IL-1β and TNF-α in BV2 cells exposed to GLUT1 inhibitors (STF-31, 5 µmol/L and BAY-876, 50 nmol/L) for 48 h with or without glucose treatment.

: STF-31 purchased from MedChemExpress. Usage Cited in: Cell Biosci. 2024 Apr 16;14(1):48. [Abstract]; Flow cytometry analysis of CD86⁺ populations in BV2 cells exposed to STF-31 (5 μM) or BAY-876 under control or glucose conditions. Representative images.

: STF-31 purchased from MedChemExpress. Usage Cited in: Cell Biosci. 2024 Apr 16;14(1):48. [Abstract]; Levels of IκBα in BV2 cells exposed to STF-31 (5 μM) or BAY-876 upon glucose treatment were determined by western blotting.

: STF-31 purchased from MedChemExpress. Usage Cited in: Cell Biosci. 2024 Apr 16;14(1):48. [Abstract]; NF-κB nuclear translocation in BV2 cells exposed to STF-31 (5 μM) or BAY-876 under glucose incubation was analyzed by IF staining.

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View All Isoforms

GLUT1 GLUT2 GLUT3 GLUT4

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[2]

GLUT1

1 μM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	0.12 μM Compound: 51	Antiproliferative activity against human A2780 cells after 72 hrs by SRB assay Antiproliferative activity against human A2780 cells after 72 hrs by SRB assay	[PMID: 24433859]
A2780	IC₅₀	120 nM Compound: 76; STF-31	Antiproliferative activity against human A2780 cells Antiproliferative activity against human A2780 cells	[PMID: 38244254]
A2780	IC₅₀	24 nM Compound: 51	Inhibition of NAMPT in human A2780 cells assessed as reduction in NAD level after 48 hrs by LC-MS/MS analysis Inhibition of NAMPT in human A2780 cells assessed as reduction in NAD level after 48 hrs by LC-MS/MS analysis	[PMID: 24433859]
HCT-116	IC₅₀	271 nM Compound: 51	Antiproliferative activity against human HCT116 cells after 96 hrs by CyQuant-based assay Antiproliferative activity against human HCT116 cells after 96 hrs by CyQuant-based assay	[PMID: 24433859]
HT-1080	IC₅₀	213 nM Compound: 51	Antiproliferative activity against human HT1080 cells after 96 hrs by CyQuant-based assay Antiproliferative activity against human HT1080 cells after 96 hrs by CyQuant-based assay	[PMID: 24433859]
MIA PaCa-2	IC₅₀	855 nM Compound: 51	Antiproliferative activity against human MIAPaCa2 cells after 96 hrs by CyQuant-based assay Antiproliferative activity against human MIAPaCa2 cells after 96 hrs by CyQuant-based assay	[PMID: 24433859]
PC-3	IC₅₀	217 nM Compound: 51	Antiproliferative activity against human PC3 cells after 96 hrs by CyQuant-based assay Antiproliferative activity against human PC3 cells after 96 hrs by CyQuant-based assay	[PMID: 24433859]
RCC4	IC₅₀	0.16 μM Compound: 2	Cytotoxicity against human RCC4 cells after 4 days by XTT assay Cytotoxicity against human RCC4 cells after 4 days by XTT assay	[PMID: 24387866]
RCC4	IC₅₀	7.9 μM Compound: 33; STF-31	Growth inhibition of VHL-deficient human RCC4 cells by XTT assay Growth inhibition of VHL-deficient human RCC4 cells by XTT assay	[PMID: 28042452]
RCC4/VHL	IC₅₀	> 40 μM Compound: 2	Cytotoxicity against human RCC4/VHL cells after 4 days by XTT assay Cytotoxicity against human RCC4/VHL cells after 4 days by XTT assay	[PMID: 24387866]

In Vitro

STF-31 (0.01-10 μM; 10 days) is specifically toxic to RCC4 cells, whereas RCC4/VHL cells are relatively unaffected. RCC4/VHL cells treated with STF-31 (5 μM; 10 days) largely recovery, whereas RCC4 cells under the same conditions does not^[1].
STF-31 (1.25-5 μM; 3 days) does not induce autophagy, apoptosis, or DNA damage. STF-31 causes a necrotic cell death^[1].
STF-31 reduces the efferocytosis of wild type and SLC2A1-overexpressing LR73 cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Wild-type RCC4 cells (RCC4/VHL) and RCC4 cells without VHL
Concentration:	0.01, 0.1, 1, 10 μM
Incubation Time:	10 days
Result:	Reduced viability of RCC4 cells without VHL in a concentration-dependent manner when compared to their wild-type counterparts.

In Vivo

STF-31 (10 mg/kg; i.p.; twice daily for 2 days, followed by once daily for another 3 days) does not affect normal mice body weight, behavior, and ERG responses. STF-31 reduces light-induced CX3CR1^gfp/+ mice microglial activation and retinal degeneration^[3].
STF-31 (10 mg/kg; i.p.) promotes accumulation of necrotic thymocytes after Dexamethasone-induced apoptosis in vivo^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Twelve-week old C57BL/6J and CX3CR1^gfp/+ mice^[3]
Dosage:	10 mg/kg
Administration:	I.p. injections; twice daily for 2 days, followed by once daily for another 3 days
Result:	Improved photoreceptor survival and reduced microglial activation of CX3CR1^gfp/+ mice, and not induced any C57BL/6J mice retinal cell death.

Molecular Weight

423.53

Formula

C₂₃H₂₅N₃O₃S

CAS No.

724741-75-7

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(NC1=CC=CN=C1)C2=CC=C(CNS(=O)(C3=CC=C(C(C)(C)C)C=C3)=O)C=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 34 mg/mL (80.28 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3611 mL	11.8055 mL	23.6111 mL
5 mM	0.4722 mL	2.3611 mL	4.7222 mL
10 mM	0.2361 mL	1.1806 mL	2.3611 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (5.90 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.79%

Select Batch:

Data Sheet (277 KB) SDS (392 KB)

COA (246 KB) HNMR (404 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Chan DA, et al. Targeting GLUT1 and the Warburg effect in renal cell carcinoma by chemical synthetic lethality. Sci Transl Med. 2011 Aug 3;3(94):94ra70. [Content Brief]

[2]. Dedda CD, et al. Pharmacological Targeting of GLUT1 to Control Autoreactive T Cell Responses. International Journal of Molecular Sciences. 2019 Oct 8; 20(19):4962. [Content Brief]

[3]. Wang L, et al. Glucose transporter 1 critically controls microglial activation through facilitating glycolysis. Molecular Neurodegeneration, 2019 Jan 11; 14(1):2. [Content Brief]

[4]. Morioka S, et, al. Efferocytosis induces a novel SLC program to promote glucose uptake and lactate release. Nature. 2018 Nov;563(7733):714-718. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3611 mL	11.8055 mL	23.6111 mL	59.0277 mL
	5 mM	0.4722 mL	2.3611 mL	4.7222 mL	11.8055 mL
	10 mM	0.2361 mL	1.1806 mL	2.3611 mL	5.9028 mL
	15 mM	0.1574 mL	0.7870 mL	1.5741 mL	3.9352 mL
	20 mM	0.1181 mL	0.5903 mL	1.1806 mL	2.9514 mL
	25 mM	0.0944 mL	0.4722 mL	0.9444 mL	2.3611 mL
	30 mM	0.0787 mL	0.3935 mL	0.7870 mL	1.9676 mL
	40 mM	0.0590 mL	0.2951 mL	0.5903 mL	1.4757 mL
	50 mM	0.0472 mL	0.2361 mL	0.4722 mL	1.1806 mL
	60 mM	0.0394 mL	0.1968 mL	0.3935 mL	0.9838 mL
	80 mM	0.0295 mL	0.1476 mL	0.2951 mL	0.7378 mL

STF-31 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

STF-31724741-75-7STF31STF 31GLUTAutophagyGlucose transporterinhibitorglucoseGLUT1RCC4/VHLRCC4VHLInhibitorinhibit

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STF-31

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STF-31 Related Antibodies

17 Publications Citing Use of MCE STF-31

Featured Recommendations

•Related Screening Libraries:

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•Related Proteins:

View All GLUT Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

STF-31 Related Antibodies

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Complete Stock Solution Preparation Table

STF-31 Related Classifications

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