2. Anti-infection Metabolic Enzyme/Protease PI3K/Akt/mTOR
  3. Antibiotic Bacterial Dihydrofolate reductase (DHFR) mTOR
  4. Succinylsulfathiazole

Succinylsulfathiazole  (Synonyms: Succinylsulphathiazole)

Cat. No.: HY-B0921 Purity: 99.22%
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Succinylsulfathiazole (Succinylsulphathiazole) is a long-acting sulfonamide antibiotic with localized gut-specific antibacterial activity and is orally active. Succinylsulfathiazole inhibits bacterial folate synthesis, reduces coliform counts, suppresses intestinal bacterial growth and vitamin biosynthesis, and depletes gut folate-producing bacteria. Succinylsulfathiazole modulates hepatic mTOR signaling, diminishes cecal fermentation, decreases hepatic folate levels and total folate excretion, elevates nitrogen excretion and reduces the fermentability of certain dietary fibers. Succinylsulfathiazole induces folate deficiency and triggers biotin- and folate-related nutritional deficiency symptoms in rats and C57BL/6 mice.

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Succinylsulfathiazole

Succinylsulfathiazole Chemical Structure

CAS No. : 116-43-8

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Based on 1 publication(s) in Google Scholar

Other Forms of Succinylsulfathiazole:

Succinylsulfathiazole Related Antibodies

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1 Publications Citing Use of MCE Succinylsulfathiazole

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Succinylsulfathiazole (Succinylsulphathiazole) is a long-acting sulfonamide antibiotic with localized gut-specific antibacterial activity and is orally active. Succinylsulfathiazole inhibits bacterial folate synthesis, reduces coliform counts, suppresses intestinal bacterial growth and vitamin biosynthesis, and depletes gut folate-producing bacteria. Succinylsulfathiazole modulates hepatic mTOR signaling, diminishes cecal fermentation, decreases hepatic folate levels and total folate excretion, elevates nitrogen excretion and reduces the fermentability of certain dietary fibers. Succinylsulfathiazole induces folate deficiency and triggers biotin- and folate-related nutritional deficiency symptoms in rats and C57BL/6 mice[1][2][3].

IC50 & Target[1]

mTOR

 

In Vivo

Succinylsulfathiazole (2%; 119 days) causes a significant, variable reduction in fecal coliform counts but no significant changes in total aerobic organisms, total anaerobic organisms, or anaerobic spore counts in Sunny Hill strain piebald rats[3].
Succinylsulfathiazole (2%) reduces fecal excretion of biotin, folic acid, and pantothenic acid compared to unsupplemented stock or purified diets in Sunny Hill strain piebald rats[3].
Succinylsulfathiazole (2%; 3 days) reduces fecal coliform counts, with no significant changes in total aerobic organisms, total anaerobic organisms, or anaerobic spore counts in Albino Farms strain rats[3].
Succinylsulfathiazole (1%; oral consumption; daily; 21 weeks) modulates key components of the PI3K/AKT/mTOR signaling pathway and energy metabolism regulators in C57BL/6 mouse liver (with differential effects on REDD1 expression in folate adequate vs. depleted mice)[1].
Succinylsulfathiazole (5 g/kg; oral; ad libitum; 27-29 days) reduces fecal folate excretion, lowers liver folate stores, decreases markers of bacterial growth, and alters cecal short-chain fatty acid production in folate-depleted Sprague-Dawley rats during folate repletion[2].
Succinylsulfathiazole (2% w/w; oral; continuous feeding; 119 days) reduces fecal coliform counts, lowers fecal biotin and folic acid excretion, and induces leukopenia[3].
Succinylsulfathiazole (0.5-2% w/w; oral; continuous feeding) reduces fecal coliform counts[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 (male, specific pathogen-free, 6 weeks at purchase, studied at 4 months)[1]
Dosage: 1% (incorporated into purified isoenergetic diets, folate adequate: 2 mg folate/kg diet; folate depleted: 0 mg folate/kg diet)
Administration: oral consumption; daily; 21 weeks
Result: Showed no significant difference in body weight gain or food consumption over 21 weeks.
Exhibited no significant differences in folate levels across serum, liver and colon tissues versus untreated controls.
In folate-adequate mice: significantly reduced PDK1 expression and mTORc1 phosphorylation at Ser2448, elevated REDD1 expression, and lowered the ADP/ATP ratio and AMPK phosphorylation.
In folate-depleted mice: significantly decreased REDD1 expression, the ADP/ATP ratio and AMPK phosphorylation.
Reduced 4E-BP1 phosphorylation in both dietary groups.Showed no significant effects on AKT expression, mTORc1 autophosphorylation at Ser2481, S6K1 phosphorylation, autophagy-related proteins (Beclin, ATG5, LC3 cleavage), the NAD+/NADH ratio or SIRT1 expression in either group.
Animal Model: Sprague-Dawley (male weanling, initial weight 45.3 g, folate-depleted by low-folacin AIN93G basal diet for 28 days)[2]
Dosage: 5 g/kg diet
Administration: oral; ad libitum; 27-29 days
Result: Reduced total folate excretion from 13.8 to 4.8 nmol/d (pooled diets).
Lowered mean liver folate concentration from 11.6 to 7.5 nmol/g (pooled diets); this reduction was significant in rats fed standard, ground corn, wheat germ, inulin and polydextrose diets, but not in those fed wheat bran or oat bran diets.
Decreased diaminopimelic acid and muramic acid excretion, and increased nitrogen excretion (pooled diets).
Reduced fermentability of oat bran, ground corn, and wheat germ fibers; had no effect on wheat bran fiber, inulin, or polydextrose fermentability.
Lowered cecal acetic acid and propionic acid contents overall, with a diet×antibiotic interaction for propionic acid.
Animal Model: Sunny Hill strain piebald rats (~75 days old when placed on succinylsulfathiazole diet)[3]
Dosage: 2% w/w
Administration: oral; continuous feeding; 119 days
Result: Dropped fecal coliform counts immediately, with one rat averaging 105 per gram feces, another averaging 103 per gram feces with wide fluctuation (0-108), and the third having feces almost entirely devoid of coliforms until near the end of the experiment when counts rose to 104 per gram.
Showed no significant change in total aerobic and anaerobic organism counts, and anaerobic spore counts compared to pre-drug diet levels.
Reduced fecal excretion of biotin to 0.28 μg/gm feces and 0.39 μg/day, and folic acid to 2.5 μg/gm feces and 4.2 μg/day, significantly lower than in rats fed stock or purified diets without succinylsulfathiazole.
Induced low total leucocyte counts prior to vitamin supplementation.
Animal Model: Albino Farms strain rats (~60 days old when placed on succinylsulfathiazole diet)[3]
Dosage: 0.5% w/w; 2% w/w
Administration: oral; continuous feeding
Result: Reduced fecal coliform counts to a low level with 2% w/w diet, though this reduction occurred more slowly than with an equivalent level of phthalylsulfathiazole.
Resulted in fecal coliform counts < 104 per gram feces after 3 days of feeding with 0.5% w/w diet.
Showed no significant change in total aerobic and anaerobic organism counts, and anaerobic spore counts compared to pre-drug diet levels.
Molecular Weight

355.39

Formula

C13H13N3O5S2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(O)CCC(NC1=CC=C(S(=O)(NC2=NC=CS2)=O)C=C1)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (281.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 1 mg/mL (2.81 mM; ultrasonic and warming and heat to 80°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8138 mL 14.0691 mL 28.1381 mL
5 mM 0.5628 mL 2.8138 mL 5.6276 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.03 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (7.03 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
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Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.22%

SDS (393 KB)

COA (247 KB) HNMR (260 KB) LCMS (100 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.8138 mL 14.0691 mL 28.1381 mL 70.3453 mL
DMSO 5 mM 0.5628 mL 2.8138 mL 5.6276 mL 14.0691 mL
10 mM 0.2814 mL 1.4069 mL 2.8138 mL 7.0345 mL
15 mM 0.1876 mL 0.9379 mL 1.8759 mL 4.6897 mL
20 mM 0.1407 mL 0.7035 mL 1.4069 mL 3.5173 mL
25 mM 0.1126 mL 0.5628 mL 1.1255 mL 2.8138 mL
30 mM 0.0938 mL 0.4690 mL 0.9379 mL 2.3448 mL
40 mM 0.0703 mL 0.3517 mL 0.7035 mL 1.7586 mL
50 mM 0.0563 mL 0.2814 mL 0.5628 mL 1.4069 mL
60 mM 0.0469 mL 0.2345 mL 0.4690 mL 1.1724 mL
80 mM 0.0352 mL 0.1759 mL 0.3517 mL 0.8793 mL
100 mM 0.0281 mL 0.1407 mL 0.2814 mL 0.7035 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Keywords:

Succinylsulfathiazole116-43-8SuccinylsulphathiazoleAntibioticBacterialDihydrofolate reductase (DHFR)mTORMammalian target of RapamycinAlbino Farms strain ratsPI3K/AKT/mTOR signaling pathwaySprague-Dawley ratsSunny Hill strain piebald ratscoliform bacteriasulfonamide antibioticmTOR signaling pathwayfolate synthesisc57Bl/6 miceintestinal bacteriaInhibitorinhibitorinhibit

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