(2R,6R)-Hydroxynorketamine hydrochloride
Based on 1 Customer Validation
(2R,6R)-Hydroxynorketamine ((2R,6R)-HNK) hydrochloride is an active ketamine metabolite with no NMDAR binding activity. (2R,6R)-Hydroxynorketamine hydrochloride rescues impaired dorsal hippocampal long-term potentiation and restores robust long-term potentiation in the hippocampal SC-CA1 pathway. (2R,6R)-Hydroxynorketamine hydrochloride can be used for research on depression.
For research use only. We do not sell to patients.
- Purity: 99.91%
- CAS No.: 1430202-69-9
- Formula: C12H15Cl2NO2
- Molecular Weight:276.16
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Wistar-Kyoto (WKY) (male, 10-12 weeks old)[1]
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Dosage:5 mg/kg
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Administration:i.p.; single injection
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Result:Had no significant effect on basal dorsal hippocampal synaptic transmission in WKY rats.
Enhanced potentiation immediately post-high-frequency stimulation of sLTP in dorsal hippocampal Schaffer collateral-CA1 pathway compared to saline-treated controls at 3.5 hours post-injection.
Maintained robust sLTP (normalized fEPSP slope significantly above pre-induction levels) in 88% of treated rats at 90 minutes post-induction, compared to 21% of saline-treated rats.
Significantly reversed long-term spatial memory deficit (novel object location recognition test at 24 hours delay) to levels comparable to control Wistar rats, with statistically significant difference vs saline-treated WKY rats.
Had no significant effect on forced swim test immobility time at 30 minutes or 24 hours post-injection.
Chemical Information
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CAS No. 1430202-69-9
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Appearance Solid
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Molecular Weight 276.16
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Formula C12H15Cl2NO2
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Color White to light yellow
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SMILES
N[C@@]1(C([C@@H](CCC1)O)=O)C2=C(C=CC=C2)Cl.Cl
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Synonyms
(2R,6R)-HNK hydrochloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : ≥ 100 mg/mL (362.11 mM)
DMSO : 100 mg/mL (362.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.05 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.05 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (281 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.6211 mL | 18.1054 mL | 36.2109 mL | 90.5272 mL |
| 5 mM | 0.7242 mL | 3.6211 mL | 7.2422 mL | 18.1054 mL | |
| 10 mM | 0.3621 mL | 1.8105 mL | 3.6211 mL | 9.0527 mL | |
| 15 mM | 0.2414 mL | 1.2070 mL | 2.4141 mL | 6.0351 mL | |
| 20 mM | 0.1811 mL | 0.9053 mL | 1.8105 mL | 4.5264 mL | |
| 25 mM | 0.1448 mL | 0.7242 mL | 1.4484 mL | 3.6211 mL | |
| 30 mM | 0.1207 mL | 0.6035 mL | 1.2070 mL | 3.0176 mL | |
| 40 mM | 0.0905 mL | 0.4526 mL | 0.9053 mL | 2.2632 mL | |
| 50 mM | 0.0724 mL | 0.3621 mL | 0.7242 mL | 1.8105 mL | |
| 60 mM | 0.0604 mL | 0.3018 mL | 0.6035 mL | 1.5088 mL | |
| 80 mM | 0.0453 mL | 0.2263 mL | 0.4526 mL | 1.1316 mL | |
| 100 mM | 0.0362 mL | 0.1811 mL | 0.3621 mL | 0.9053 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
(2R,6R)-Hydroxynorketamine hydrochloride Related Classifications
HY-101955A Related Classifications
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- (2R,6R)-Hydroxynorketamine
- 1430202-69-9
- (2R,6R)-HNK
- Drug Metabolite
- Schaffer collateral-CA1 sLTP
- dorsal hippocampal long-term potentiation
- NMDAR
- treatment-resistant depression
- forced swim test
- hippocampal-dependent long-term spatial memory
- depression
- hippocampal SC-CA1 strong long-term potentiation
- male WKY rats
- Inhibitor
- inhibitor
- inhibit