alpha-1,3-Galactosyltransferase (a1,3GalT)
Based on 1 Customer Validation
alpha-1,3-Galactosyltransferase (a1,3GalT) (GGTA1) catalyzes the generation of the α-gal glycan via the transfer of a galactose (Gal) in α1-3 linkage, from a uridyl-diphosphate (UDP) donor onto the N-acetyllactosamine (Galβ1,4GlcNAc-R) of glycoproteins. alpha-1,3-Galactosyltransferase (a1,3GalT) is responsible for the synthesis of the α-galactose (α-Gal) epitope found in most mammalian species.
For research use only. We do not sell to patients.
- CAS No.: 128449-51-4
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Technical Parameters
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
All Endogenous Metabolite Isoforms
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Biological Activity
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Human Endogenous Metabolite |
Product Information
This product is expressed by recombinant E. coli, N-terminal His Tagged, with a molecular weight of 36 kDa. It is used to connect galactose to the non-reducing end of the sugar chain of Galβ1,4GlcOR in an α1,3 connection manner. It is a key enzyme that catalyzes the synthesis of 3-sugar.
Instructions
1. Add substrate according to the reaction system, 10 mM MnCl2, and buffer salt (such as Tris-HCl) can be appropriately added to adjust the pH to 7.5, and add an appropriate amount of α1,3GalT (according to reaction requirements)
2. After mixing, incubate the sample at 37°C for 2-4 hours, or overnight.
Notes
When the system is scaled up, the incubation time or enzyme amount needs to be adjusted according to the actual situation, and can be mixed by low-speed shaking.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
2.4.1.87
≥ 1 U/mg soild
One unit is defined as the amount of enzyme that transfer 1 μmol Gal from UDP-Gal to lactose to form Galα1,3Lac per min at 37°C
Technical Parameters
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Source
E. coli
Chemical Information
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CAS No. 128449-51-4
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[alpha-1,3-Galactosyltransferase(a1,3GalT)]
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Synonyms
GGTA1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (268 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Sumnima Singh, et al. Loss of α-gal during primate evolution enhanced antibody-effector function and resistance to bacterial sepsis. Cell Host Microbe. 2021 Mar 10;29(3):347-361.e12. [Content Brief]
[2]. Anne-Laure Turcot-Dubois, et al. Long-term evolution of the CAZY glycosyltransferase 6 (ABO) gene family from fishes to mammals--a birth-and-death evolution model. Glycobiology. 2007 May;17(5):516-28. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
alpha-1,3-Galactosyltransferase (a1,3GalT) Related Classifications
HY-E70052 Related Classifications
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