2. Immunology/Inflammation
  3. CD19
  4. Anti-Mouse CD19 Antibody (1D3)

Anti-Mouse CD19 Antibody (1D3) 

Cat. No.: HY-P99976 Purity: 98.778%
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Anti-Mouse CD19 Antibody (1D3) is an IgG2a antibody inhibitor targeting mouse CD19. Anti-Mouse CD19 Antibody (1D3) reduces CD19 expression levels on the surface of B cells. Anti-Mouse CD19 Antibody (1D3) inhibits the replication of peritoneal B-1a cells.

For research use only. We do not sell to patients.

Anti-Mouse CD19 Antibody (1D3)

Anti-Mouse CD19 Antibody (1D3) Chemical Structure

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Description

Anti-Mouse CD19 Antibody (1D3) is an IgG2a antibody inhibitor targeting mouse CD19. Anti-Mouse CD19 Antibody (1D3) reduces CD19 expression levels on the surface of B cells. Anti-Mouse CD19 Antibody (1D3) inhibits the replication of peritoneal B-1a cells[1].

Isotype

Rat IgG2a kappa
Recommend Isotype Controls
Species Reactivity

Mouse
In Vitro

Anti-Mouse CD19 Antibody (1D3) specifically recognizes and precipitates mouse CD19 in transfected K562 cells and BALB/c splenocytes[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-Mouse CD19 Antibody (1D3) Related Antibodies
In Vivo

Anti-Mouse CD19 Antibody (1D3) (400-500 μg; i.p.) downregulates CD19 expression on mouse B cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c (timed pregnant, neonatal pups, 8-week-old adults, 6-month-old adults); BALB/c.nu (8-12-week-old, 12-week-old)[1]
Dosage: 400 μg (immune response study); 500 μg (perinatal study, adult B-1a maintenance study, B-1a replication study)
Administration: i.p.; every other day (gestation day 9 until birth for pregnant mice; 4 to 32 days for 8-week-old BALB/c and 8-12-week-old BALB/c.nu mice; 8 days for 6-month-old BALB/c mice; 12 days for 12-week-old BALB/c.nu mice); single dose at 10 days of age for pups
Result: Down-regulated CD19 expression on B cells.
Reduced splenic and peritoneal B-1a cells at 4, 9, and 23 days of age in neonates.
Reduced B-2 cells by 40% at day 4, which recovered to 92-98% of control levels by days 9 and 23 in neonates.
Reduced splenic T cells by 46% at day 4, which recovered by day 23 in neonates.
Reduced peritoneal B-1a cells gradually to 40% of control levels after 4.5 weeks of adult treatment.
Left splenic B-2 cell recovery and bone marrow B cell precursor populations in BALB/c.nu mice unchanged.
Reduced total peritoneal B-1a cells to 73% of control levels in 6-month-old mice.
Reduced BrdUrd-positive B-1a cells by 60%, while leaving BrdUrd-negative B-1a cell numbers unchanged.
Gene ID

12478  [NCBI]

Accession

P25918
Application

in vivo B cell depletion; in vivo CD19 neutralization; in vitro B cell negative selection; Flow c
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Molecular Weight

150 kDa

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Anti-Mouse CD19 Antibody (1D3)]
Formulation

Please refer to the lot-specific COA for specific buffer information.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation

Purity: 98.778%

SDS (251 KB)

COA (232 KB) SDS-PAGE (101 KB) SEC-HPLC (160 KB)

Inhibitory Antibodies User Guide (603 KB)
References
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Anti-Mouse CD19 Antibody (1D3) Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse CD19 Antibody (1D3)CD19BALB/c splenocytessplenic B-2 cellmouse CD19B cellsadult miceB-1a cellneonatal miceK562 cellsmurine T cellTNP-FicollInhibitorinhibitorinhibit

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