Copy Link Print Information

CD19 Antibody (YA807)(PBS only)

(Synonyms: CD19; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD19)

CD19 Antibody (YA807) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD19.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    ICC/IF, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • CD19 Antibody (YA807)(PBS only) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:50-1:200 1:50-1:100

Product Details

Description

CD19 Antibody (YA807) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD19.

  • Host Mouse
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 95 kDa0 Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 61 kDa
Immunogen

Synthetic peptide corresponding to Human CD19.The exact sequence is proprietary to MCE.

Purification

affinity purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    CD19 is a 95 kD transmembrane glycoprotein that is highly expressed in human B cells and certain types of tumors, belonging to the immunoglobulin superfamily. CD19 plays a crucial role in regulating the threshold of B cell signaling by modulating both B cell receptor (BCR) -dependent and independent signaling pathways, thereby playing an essential role in the initiation of immune responses and the maintenance of tolerance. CD19 typically forms a multimolecular complex with molecules like CD21 and CD81 on the surface of mature B cells, where it exerts its dominant signaling function.
    CD19 works in cooperation with the BCR to promote the activation of Src family protein tyrosine kinases, enhancing B cell proliferation and expansion. It can also independently enhance B cell activation by binding to the complement receptor CD21. Mice and humans lacking CD19 show weakened T cell-dependent immune responses and low levels of immunoglobulins, leading to immune dysfunction. High expression of CD19 is closely associated with B cell lymphoma, acute lymphoblastic leukemia (ALL), and other malignancies. CD19 monoclonal antibodies are used in the study of B cell malignancies[1].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Membrane raft; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:Detected in B cells in the marginal zone of lymph nodes and germinal centers (PubMed:2463100) . Detected in blood B cells (protein levels) (PubMed:16672701, PubMed:2463100) .

  • Isoforms & Post-Translational Modification

    P15391 has 2 isomers: P15391-1: 61128 Da (predicted); P15391-2: 61200 Da (predicted).
    Phosphorylated on tyrosine following B-cell activation (PubMed:10706702, PubMed:12387743, PubMed:7684160, PubMed:7687539). Phosphorylated on tyrosine residues by LYN (PubMed:7687428). Tyrosine residues are phosphorylated sequentially after activation of the B cell receptor. Phosphorylation of Tyr-531 is extremely rapid, followed by phosphorylation at Tyr-409. In contrast, phosphorylation of Tyr-500 appears more slowly and is more transient, returning rapidly to basal levels (By similarity)

  • Subunit

    Interacts with CR2/CD21 (PubMed:1702139). Part of a complex composed of CD19, CR2/CD21, CD81 and IFITM1/CD225 in the membrane of mature B-cells (PubMed:1383329). Interacts directly with CD81 (via the second extracellular domain); this interaction is initiated early during biosynthesis in the ER/pre-Golgi compartments and is essential for trafficking and compartmentalization of CD19 receptor on the cell surface of activated B cells (PubMed:16449649). Interacts with VAV (PubMed:10706702). Interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. Interacts with PLCG2 when phosphorylated on Tyr-409 (PubMed:10706702). Interacts with LYN (PubMed:7687428). Interacts (when tyrosine phosphorylated) with the regulatory p85 subunit of phosphatidylinositol 3-kinase (PIK3R1 or PIK3R2) (PubMed:7684160). Interacts with GRB2 (PubMed:29523808)

  • SwissProt ID

    P15391

  • Gene ID
    930 [NCBI]
  • Synonyms

    CD19; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD19

  • Research Field

    Immunology

References

[1]. Wang K, et al. CD19: a biomarker for B cell development, lymphoma diagnosis and therapy. Exp Hematol Oncol. 2012 Nov 29;1(1):36. [Content Brief]

CD19 Antibody (YA807)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • CD19 Antibody (YA807)(PBS only) Product Image
    1/16
  • CD19 Antibody (YA807)(PBS only) Product Image