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CD80 Antibody (YA3963)

(Synonyms: B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1)
1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

CD80 Antibody (YA3963) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD80.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    IHC-P, FC, ELISA

  • Reactivity :

    Human, Mouse, Rat, Rabbit

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL IHC-P FC
  • Experimental Validation Results for CD80 Antibody (YA3963)
  • Experimental Validation Results for CD80 Antibody (YA3963)
  • Experimental Validation Results for CD80 Antibody (YA3963)
  • Experimental Validation Results for CD80 Antibody (YA3963)
  • Experimental Validation Results for CD80 Antibody (YA3963)
  • Experimental Validation Results for CD80 Antibody (YA3963)
  • CD80 Antibody (YA3963) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:100-1:500 1:200-1:400 1:10000

Product Details

Description

CD80 Antibody (YA3963) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD80.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Rabbit
  • Observed Molecular Weight
    Observed band size: 50-75 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 33 kDa
Immunogen

Purified recombinant fragment of human CD80 (AA: 35-288) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD80 Antibody (YA3963)
    Immunohistochemical analysis of paraffin-embedded human uterine appendages tissue using CD80 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84266, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD80 Antibody (YA3963)
    Immunohistochemical analysis of paraffin-embedded human mesothelioma tissue using CD80 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84266, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD80 Antibody (YA3963)
    Immunohistochemical analysis of paraffin-embedded human esophagus tissue using CD80 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84266, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD80 Antibody (YA3963)
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using CD80 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84266, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD80 Antibody (YA3963)
    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using CD80 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84266, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD80 Antibody (YA3963)
    Flow cytometric analysis of 1X106 C2C12 cells labeling CD80 Antibody (HY-P84266, red). Cells were stained with the primary antibody at 1/400 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    CD28/CD80 is a co-stimulatory molecule belonging to the immunoglobulin superfamily that plays a crucial role in T-lymphocyte activation. CD80 interacts with the CD28 receptor-constitutively expressed on the surface of T cells-to serve as a primary co-stimulatory signal that enhances MHC/TCR signaling in naive T cells. This triggers the activation of various signaling pathways, such as NF-κB or MAPK, ultimately leading to the production of multiple cytokines. Furthermore, the CD28/CD80 co-stimulatory signal promotes T-cell glucose metabolism and ATP synthesis by activating the PI3K/Akt signaling pathway. CD80 also acts as a regulator of the PD-L1/PDCD1 interaction, limiting the excessive activation of PD-L1 and its inhibitory effects during immune responses. Expressed on the surface of B cells, CD80 plays a pivotal regulatory role in the interactions between B cells and T cells during both early and late germinal center reactions.

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:It is expressed on activated B cells, macrophages, and dendritic cells.

  • Isoforms & Post-Translational Modification

    CD80 has 3 isomers: P33681-1: 33048 Da (predicted); P33681-2: 29413 Da (predicted); P33681-3: 18761 Da (predicted).
    Palmitoylated by ZDHHC20; palmitoylation protects CD80 from ubiquitin-mediated degradation, regulating the protein stability, and ensures its accurate plasma membrane localization

  • Subunit

    Homodimer. Interacts with CTLA4; this interaction inhibits T-cell activation. Interacts with PDL1/CD274; this interaction blocks PDL1/PDCD1 binding and thus PDL1/CD274 inhibitory function. Interacts with CD28

  • SwissProt ID

    P33681

  • Gene ID
    941 [NCBI]
  • Synonyms

    B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1

[1]. Lu B, et al. zDHHC20-driven S-palmitoylation of CD80 is required for its costimulatory function. Acta Pharmacol Sin. 2024 Jun;45(6):1214-1223. [Content Brief]

[2]. Collins AV, et al. The interaction properties of costimulatory molecules revisited. Immunity. 2002 Aug;17(2):201-10. [Content Brief]

[3]. Boulougouris G, et al. IL-2-independent activation and proliferation in human T cells induced by CD28. J Immunol. 1999 Aug 15;163(4):1809-16. [Content Brief]

[4]. Frauwirth KA, et al. The CD28 signaling pathway regulates glucose metabolism. Immunity. 2002 Jun;16(6):769-77. [Content Brief]

[5]. Kennedy A, et al. The CTLA-4 immune checkpoint protein regulates PD-L1:PD-1 interaction via transendocytosis of its ligand CD80. EMBO J. 2023 Mar 1;42(5):e111556. [Content Brief]

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CD80 Antibody (YA3963) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human uterine appendages tissue using CD80 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84266, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human mesothelioma tissue using CD80 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84266, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human esophagus tissue using CD80 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84266, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using CD80 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84266, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using CD80 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84266, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 C2C12 cells labeling CD80 Antibody (HY-P84266, red). Cells were stained with the primary antibody at 1/400 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD80 Antibody (YA3963) Product Image
    1/16
  • Experimental Validation Results for CD80 Antibody (YA3963)
  • Experimental Validation Results for CD80 Antibody (YA3963)
  • Experimental Validation Results for CD80 Antibody (YA3963)
  • Experimental Validation Results for CD80 Antibody (YA3963)
  • Experimental Validation Results for CD80 Antibody (YA3963)
  • Experimental Validation Results for CD80 Antibody (YA3963)
  • CD80 Antibody (YA3963) Product Image
  • Experimental Validation Results for CD80 Antibody (YA3963)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human uterine appendages tissue using CD80 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84266, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD80 Antibody (YA3963)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human mesothelioma tissue using CD80 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84266, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD80 Antibody (YA3963)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human esophagus tissue using CD80 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84266, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD80 Antibody (YA3963)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using CD80 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84266, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD80 Antibody (YA3963)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using CD80 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84266, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD80 Antibody (YA3963)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 C2C12 cells labeling CD80 Antibody (HY-P84266, red). Cells were stained with the primary antibody at 1/400 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CD80 Antibody (YA3963)
  • Experimental Validation Results for CD80 Antibody (YA3963)
  • Experimental Validation Results for CD80 Antibody (YA3963)
  • Experimental Validation Results for CD80 Antibody (YA3963)
  • Experimental Validation Results for CD80 Antibody (YA3963)
  • Experimental Validation Results for CD80 Antibody (YA3963)
  • CD80 Antibody (YA3963) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human uterine appendages tissue using CD80 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84266, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD80 Antibody (YA3963) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human mesothelioma tissue using CD80 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84266, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD80 Antibody (YA3963) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human esophagus tissue using CD80 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84266, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD80 Antibody (YA3963) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using CD80 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84266, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD80 Antibody (YA3963) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using CD80 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P84266, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD80 Antibody (YA3963) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 C2C12 cells labeling CD80 Antibody (HY-P84266, red). Cells were stained with the primary antibody at 1/400 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD80 Antibody (YA3963) Product Image IHC-P
  • CD80 Antibody (YA3963) Product Image IHC-P
  • CD80 Antibody (YA3963) Product Image IHC-P
  • CD80 Antibody (YA3963) Product Image IHC-P
  • CD80 Antibody (YA3963) Product Image IHC-P
  • CD80 Antibody (YA3963) Product Image FC