DPH2 Antibody (YA8482)

Cat. No.: HY-P88798: Data Sheet User Guide for Antibodies
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DPH2 Antibody (YA8482) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to DPH2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

DPH2 Antibody (YA8482) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to DPH2.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 51.9 kDa

Species Reactivity

Human, Rat

SwissProt ID

Q9BQC3

Immunogen

Full length human recombinant protein of human DPH2 produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2 (PubMed:32576952). DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase (By similarity). Facilitates the reduction of the catalytic iron-sulfur cluster found in the DPH1 subunit (By similarity)

Expression：
Tissue_Specificity: Strongly expressed in skeletal muscle. Moderately expressed in heart, small intestine, liver, pancreas, testis and colon. Weakly expressed in brain, placenta, kidney, spleen, thymus, prostate, ovary and lymphocytes

Isoforms & Post-Translational Modification：Q9BQC3 has three isomers: Q9BQC3-1: 52083 Da (predicted); Q9BQC3-2: 27529 Da (predicted); Q9BQC3-3: 38510 Da (predicted).

Subunit：Component of the 2-(3-amino-3-carboxypropyl)histidine synthase complex composed of DPH1, DPH2, DPH3 and a NADH-dependent reductase (By similarity)

Synonyms

DPH2L2

Documentation

User Guide for Antibodies (1077 KB)