DPP9 Antibody (YA7755)(PBS only)

Cat. No.: HY-P88071A: Data Sheet User Guide for Antibodies
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DPP9 Antibody (YA7755) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to DPP9.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

DPP9 Antibody (YA7755) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to DPP9.

Host

Mouse

Molecular Weight

Predicted band size: 96.4 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q86TI2

Immunogen

Full length human recombinant protein of human DPP9 produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2 (PubMed:12662155, PubMed:16475979, PubMed:19667070, PubMed:29382749, PubMed:30291141, PubMed:33731929, PubMed:36112693). Acts as a key inhibitor of caspase-1-dependent monocyte and macrophage pyroptosis in resting cells by preventing activation of NLRP1 and CARD8 (PubMed:27820798, PubMed:29967349, PubMed:30291141, PubMed:31525884, PubMed:32796818, PubMed:36112693, PubMed:36357533). Sequesters the cleaved C-terminal part of NLRP1 and CARD8, which respectively constitute the active part of the NLRP1 and CARD8 inflammasomes, in a ternary complex, thereby preventing their oligomerization and activation (PubMed:33731929, PubMed:33731932, PubMed:34019797). The dipeptidyl peptidase activity is required to suppress NLRP1 and CARD8; however, neither NLRP1 nor CARD8 are bona fide substrates of DPP9, suggesting the existence of substrate(s) required for NLRP1 and CARD8 inhibition (PubMed:33731929)

Subcellular Localization：Cytoplasm, cytosol,Nucleus

Expression：
Tissue_Specificity: Ubiquitously expressed, with highest levels in liver, heart and muscle, and lowest levels in brain

Isoforms & Post-Translational Modification：Q86TI2 has three isomers: Q86TI2-1: 98263 Da (predicted); Q86TI2-2: 101669 Da (predicted); Q86TI2-4: 95013 Da (predicted).

Subunit：Homodimer (PubMed:16475979, PubMed:29382749)

Synonyms

DP9; DPLP9; DPRP-2; DPRP2

Documentation

User Guide for Antibodies (1077 KB)