IFI35 Antibody (YA8137)
(Synonyms: IFP35)IFI35 Antibody (YA8137) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to IFI35.
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Host:
Mouse
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Isotype:
IgG2a
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Application:
IHC-P, ICC/IF, FC
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Reactivity :
Human
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Formulation:
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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|---|---|---|---|
| Dilution Ratio | 1:150-500 | 1:100-250 | 1:100 |
Product Details
IFI35 Antibody (YA8137) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to IFI35.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 31.6 kDa
Full length human recombinant protein of human IFI35produced in HEk293T cell
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG2a
Product Properties
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Appearance
Liquid
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Formulation
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
IFI35 acts as a signaling pathway regulator involved in innate immune system response. In response to interferon IFN-alpha, associates in a complex with signaling pathway regulator NMI to regulate immune response; the complex formation prevents proteasome-mediated degradation of IFI35 and correlates with IFI35 dephosphorylation. In complex with NMI, inhibits virus-triggered type I interferon/IFN-beta production. In complex with NMI, negatively regulates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and transcription of the NF-kappa-B subunit p65/RELA, resulting in the inhibition of endothelial cell proliferation, migration and re-endothelialization of injured arteries. Beside its role as an intracellular signaling pathway regulator, also functions extracellularly as damage-associated molecular patterns (DAMPs) to promote inflammation when actively released by macrophage to the extracellular space during cell injury and pathogen invasion. Macrophage-secreted IFI35 activates NF-kappa-B signaling in adjacent macrophages through Toll-like receptor 4/TLR4 activation, thereby inducing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of pro-inflammatory cytokines[1][2][3][4][5].
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Subcellular Localization
Cytoplasm,Nucleus,Secreted
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Expression
Tissue_Specificity: Expressed in a wide range of cell types, including fibroblasts, macrophages, and epithelial cells
Induction: Up-regulated by interferons IFN-alpha and IFN-gamma -
Isoforms & Post-Translational Modification
P80217 has two isomers: P80217-1: 31546 Da (predicted); P80217-2: 31777 Da (predicted).
Phosphorylated -
Subunit
Homodimer (PubMed:10950963, PubMed:8288566)
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SwissProt ID
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Synonyms
IFP35
Documentation
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