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  5. KLF4 Antibody (YA3537)(PBS only)

KLF4 Antibody (YA3537)(PBS only)

製品番号: HY-P83840A
User Guide for Antibodies Technical Support

KLF4 Antibody (YA3537) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to KLF4.

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研究用途以外に使用した場合、当社は一切の責任を負いかねます。

容量 在庫状況
50 μL   お問い合わせ  
100 μL   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • 説明

製品説明

KLF4 Antibody (YA3537) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to KLF4.
Host

Mouse
分子量
Predicted band size: 55 kDa;
Observed band size: 55/65 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

Purified recombinant fragment of human KLF4 (AA: 2-470) expressed in E. Coli.
Application &
Dilution Ratio
Application Dilution Ratio
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:200-1:1000
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
1:10000
純度 affinity purified. Conjugation Non-conjugated
Isotype IgG1  
Appearance

Liquid
Formulation

Supplied in PBS, pH 7.4.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
輸送条件

Shipping with blue ice.
Background
Function：Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription
Subcellular Localization：Nucleus; Cytoplasm
Isoforms & Post-Translational Modification：O43474 has 5 isomers: O43474-3: 54671 Da (predicted); O43474-1: 51076 Da (predicted); O43474-4: 45639 Da (predicted); O43474-5: 12329 Da (predicted); O43474-6: 6489 Da (predicted).
Ubiquitinated. 'Lys-48'-linked ubiquitinated and targeted for proteasomal degradation by the SCF(BTRC) E3 ubiquitin-protein ligase complex, thereby negatively regulating cell pluripotency maintenance and embryogenesis;Polyglutamylated by TTLL1 and TTLL4 at Glu-411, which inhibits KLF4 binding with E3 ligase component BTRC, thereby impeding ubiquitination. Deglutamylated by CCP1 and CCP6; deglutamylation promotes KLF4 ubiquitination. KLF4 glutamylation state plays a critical role in the regulation of its function in cell reprogramming, pluripotency maintenance and embryogenesis
Subunit：Interacts with POU5F1/OCT4 and SOX2 (By similarity). Interacts with MUC1 (via the C-terminal domain) (PubMed:17308127). Interacts with MEIS2 isoform 4 and PBX1 isoform PBX1a (PubMed:21746878). Interacts with ZNF296 (By similarity). Interacts with GLIS1 (PubMed:21654807). Interacts with BTRC; this interaction leads to KLF4 ubiquitination and subsequent degradation (By similarity). Interacts with IPO7; the interaction facilitates nuclear translocation of KLF4 in dental papilla cells (By similarity)
Synonyms
EZF; GKLF; KLF4
ドキュメンテーション
User Guide for Antibodies (1077 KB)

KLF4 Antibody (YA3537)(PBS only) 関連分類

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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製品名:
KLF4 Antibody (YA3537)(PBS only)
製品番号:
HY-P83840A
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