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KMT2C Antibody (YA4374)

(Synonyms: HALR; MLL3; KLEFS2)
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Based on 1 Customer Validation

KMT2C Antibody (YA4374) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to KMT2C.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for KMT2C Antibody (YA4374)
  • Experimental Validation Results for KMT2C Antibody (YA4374)
  • KMT2C Antibody (YA4374) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

KMT2C Antibody (YA4374) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to KMT2C.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 269 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 541 kDa
Immunogen

Purified recombinant fragment of human KMT2C (AA: 1-205) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718962

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for KMT2C Antibody (YA4374)
    Flow cytometric analysis of 1X106 HeLa cells labeling KMT2C Antibody (HY-P84677, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for KMT2C Antibody (YA4374)
    Flow cytometric analysis of 1X10^6 Hela cells labeling KMT2C Antibody(HY-P84677

Background

  • Function

    KMT2C is a Histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4). Part of chromatin remodeling machinery predominantly forms H3K4me1 methylation marks at active chromatin sites where transcription and DNA repair take place. Likely plays a redundant role with KMT2D in enriching H3K4me1 mark on primed and active enhancer elements[1][2][3].

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:Highly expressed in testis and ovary, followed by brain and liver. Also expressed in placenta, peripherical blood, fetal thymus, heart, lung and kidney. Within brain, expression was highest in hippocampus, caudate nucleus, and substantia nigra. Not detected in skeletal muscle and fetal liver

  • Isoforms & Post-Translational Modification

    Q8NEZ4 has 3 isomers: Q8NEZ4-1: 541370 Da (predicted); Q8NEZ4-2: 547117 Da (predicted); Q8NEZ4-3: 547633 Da (predicted).

  • Subunit

    Component of the MLL3 complex (also named ASCOM complex), at least composed of catalytic subunit KMT2C/MLL3, ASH2L, RBBP5, WDR5, NCOA6, DPY30, KDM6A, PAXIP1/PTIP, PAGR1 and alpha- and beta-tubulin (PubMed:17021013, PubMed:17500065, PubMed:23508102). Forms a core complex with the evolutionary conserved subcomplex WRAD composed of WDR5, RBBP5, ASH2L/ASH2 and DPY30 subunits; WRAD differentially stimulates the methyltransferase activity (PubMed:25561738). Interacts (via WIN motif) with WDR5 (PubMed:22266653, PubMed:22665483)

  • SwissProt ID

    Q8NEZ4

  • Gene ID
    58508 [NCBI]
  • Synonyms

    HALR; MLL3; KLEFS2

KMT2C Antibody (YA4374) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling KMT2C Antibody (HY-P84677, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling KMT2C Antibody(HY-P84677

  • KMT2C Antibody (YA4374) Product Image
    1/16
  • Experimental Validation Results for KMT2C Antibody (YA4374)
  • Experimental Validation Results for KMT2C Antibody (YA4374)
  • KMT2C Antibody (YA4374) Product Image
  • Experimental Validation Results for KMT2C Antibody (YA4374)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling KMT2C Antibody (HY-P84677, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for KMT2C Antibody (YA4374)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling KMT2C Antibody(HY-P84677
  • Experimental Validation Results for KMT2C Antibody (YA4374)
  • Experimental Validation Results for KMT2C Antibody (YA4374)
  • KMT2C Antibody (YA4374) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HeLa cells labeling KMT2C Antibody (HY-P84677, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • KMT2C Antibody (YA4374) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 Hela cells labeling KMT2C Antibody(HY-P84677

  • KMT2C Antibody (YA4374) Product Image FC
  • KMT2C Antibody (YA4374) Product Image FC