KMT5A Antibody (YA4515)
(Synonyms: SET8; SET07; SETD8; PR-Set7)KMT5A Antibody (YA4515) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to KMT5A.
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Host:
Mouse
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Isotype:
IgG1
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Application:
ICC/IF, FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:200-1:500 | 1:200-1:400 | 1:10000 |
Product Details
KMT5A Antibody (YA4515) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to KMT5A.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 50 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 43 kDa
Purified recombinant fragment of human KMT5A (AA: 109-272) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS with 0.05% sodium azide
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
KMT5A is a Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration[1][2][3][4][5][6][7][8][9][10].
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Subcellular Localization
Nucleus; Chromosome
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Expression
Induction:By HCFC1 C-terminal chain, independently of HCFC1 N-terminal chain. Transiently induced by TGF-beta and during the cell cycle -
Isoforms & Post-Translational Modification
Q9NQR1 has 2 isomers: Q9NQR1-1: 42890 Da (predicted); Q9NQR1-2: 39223 Da (predicted).
Acetylated at Lys-162; does not affect methyltransferase activity. Deacetylated at Lys-162 possibly by SIRT2; does not change methyltransferase activity;Ubiquitinated and degraded by the DCX(DTL) complex -
Subunit
Interacts with L3MBTL1
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SwissProt ID
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Synonyms
SET8; SET07; SETD8; PR-Set7
Documentation
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