MICAL1 Antibody (YA8441)

Cat. No.: HY-P88757: Data Sheet User Guide for Antibodies
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MICAL1 Antibody (YA8441) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MICAL1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

MICAL1 Antibody (YA8441) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MICAL1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 117.7 kDa

Species Reactivity

Human

SwissProt ID

Q8TDZ2

Immunogen

Full length human recombinant protein of human MICAL1 produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization (PubMed:29343822). In the absence of actin, it also functions as a NADPH oxidase producing H(2)O(2) (PubMed:21864500, PubMed:26845023, PubMed:29343822). Acts as a cytoskeletal regulator that connects NEDD9 to intermediate filaments. Also acts as a negative regulator of apoptosis via its interaction with STK38 and STK38L; acts by antagonizing STK38 and STK38L activation by MST1/STK4. Involved in regulation of lamina-specific connectivity in the nervous system such as the development of lamina-restricted hippocampal connections. Through redox regulation of the actin cytoskeleton controls the intracellular distribution of secretory vesicles containing L1/neurofascin/NgCAM family proteins in neurons, thereby regulating their cell surface levels (By similarity). May act as Rab effector protein and play a role in vesicle trafficking. Promotes endosomal tubule extension by associating with RAB8 (RAB8A or RAB8B), RAB10 and GRAF (GRAF1/ARHGAP26 or GRAF2/ARHGAP10) on the endosomal membrane which may connect GRAFs to Rabs, thereby participating in neosynthesized Rab8-Rab10-Rab11-dependent protein export (PubMed:32344433)

Subcellular Localization：Cytoplasm,Cytoplasm, cytoskeleton,Endosome membrane,Midbody

Expression：
Tissue_Specificity: Expressed in the thymus, lung, spleen, kidney, testis and hematopoietic cells

Isoforms & Post-Translational Modification：Q8TDZ2 has four isomers: Q8TDZ2-1: 117875 Da (predicted); Q8TDZ2-2: 108552 Da (predicted); Q8TDZ2-3: 37715 Da (predicted); Q8TDZ2-4: 119821 Da (predicted).

Subunit：Interacts with STK38 and STK38L (By similarity)

Synonyms

MICAL; MICAL-1; NICAL

Documentation

User Guide for Antibodies (1077 KB)