Neurofilament Heavy Polypeptide Antibody (YA3769)
(Synonyms: NFH; CMT2CC)Neurofilament Heavy Polypeptide Antibody (YA3769) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Neurofilament Heavy Polypeptide.
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Host:
Mouse
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Isotype:
IgG1
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Application:
IHC-P, FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
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Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
Neurofilament Heavy Polypeptide Antibody (YA3769) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Neurofilament Heavy Polypeptide.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 180-220 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 112 kDa
Purified recombinant fragment of human NEFH (AA: 2-251) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS with 0.05% sodium azide
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Neurofilament Heavy Polypeptide is a Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks
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Subcellular Localization
Cytoplasm, cytoskeleton; Cell projection, axon
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Isoforms & Post-Translational Modification
P12036 has 2 isomers: P12036-1: 111838 Da (predicted); P12036-2: 104998 Da (predicted).
There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber;Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function;Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization -
Subunit
Forms heterodimers with NEFL; which can further hetero-oligomerize (in vitro) (By similarity). Forms heterodimers with INA (in vitro) (By similarity)
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SwissProt ID
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Synonyms
NFH; CMT2CC
Documentation
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