Neurofilament Heavy Polypeptide Antibody (YA3769)

Art. -Nr.: HY-P84072: Data Sheet User Guide for Antibodies
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Neurofilament Heavy Polypeptide Antibody (YA3769) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Neurofilament Heavy Polypeptide.

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Größe	Verfügbarkeit	Menge
20 μL	Angebot einholen 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Angebot einholen 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Angebot einholen 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Angebot einholen

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Beschreibung

Beschreibung

Neurofilament Heavy Polypeptide Antibody (YA3769) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Neurofilament Heavy Polypeptide.

Host

Mouse

Clonality

Monoclonal Antibody

Molekulargewicht

Predicted band size: 112 kDa;

Observed band size: 180-220 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P12036

Gene ID

4744 [NCBI]

Immunogen

Purified recombinant fragment of human NEFH (AA: 2-251) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Reinheit	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Background

Function：Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity)

Subcellular Localization：Cytoplasm, cytoskeleton; Cell projection, axon

Isoforms & Post-Translational Modification：P12036 has 2 isomers: P12036-1: 111838 Da (predicted); P12036-2: 104998 Da (predicted).
There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber;Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function;Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization

Subunit：Forms heterodimers with NEFL; which can further hetero-oligomerize (in vitro) (By similarity). Forms heterodimers with INA (in vitro) (By similarity)

Synonyms

NFH; CMT2CC

Dokumentation

User Guide for Antibodies (1077 KB)