p27 KIP 1 Antibody (YA5002)
(Synonyms: CDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1)p27 KIP 1 Antibody (YA5002) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to p27 KIP 1.
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Host:
Mouse
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Isotype:
IgG1
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Application:
IHC-P; IHC-F; IF-Tissue
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Reactivity :
Human
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Formulation:
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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|---|---|---|---|
| Dilution Ratio | 1:100-500 | 1:100-500 | 1:100-500 |
Product Details
p27 KIP 1 Antibody (YA5002) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to p27 KIP 1.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 22 kDa;
Recombinant human CDKN1B. The exact sequence is proprietary to MCE.
Endogenous
affinity purified by Protein A
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
p27 KIP 1 is important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry[1].
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Subcellular Localization
Nucleus; Cytoplasm; Endosome
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Expression
Tissue_specificity:It is expressed in the kidney (protein level) (PubMed: 15509543) . It is expressed in all tissues tested (PubMed: 8033212) . The highest content is found in skeletal muscle, and the lowest content is found in the liver and kidney (PubMed: 8033212) .
Induction:Maximal levels in quiescence cells and early G (1) . Levels decrease after mitogen stimulation as cells progress toward S-phase -
Subunit
Forms a ternary complex composed of CCNE1, CDK2 and CDKN1B. Interacts directly with CCNE1; the interaction is inhibited by CDK2-dependent phosphorylation on Thr-187.
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SwissProt ID
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Synonyms
CDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1
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