PHLPP2 Antibody (YA1093)(PBS only)

Cat. No.: HY-P81348A: Data Sheet User Guide for Antibodies
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PHLPP2 Antibody (YA1093) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHLPP2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PHLPP2 Antibody (YA1093) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHLPP2.

Host

Rabbit

Molecular Weight

Predicted band size: 147 kDa;

Observed band size: 147 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q6ZVD8

Gene ID

23035 [NCBI]

Immunogen

A synthesized peptide derived from human PHLPP2

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	100-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis (PubMed:20513427). Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation (PubMed:21986499). Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates RAF1 inhibiting its kinase activity (PubMed:24530606)

Subcellular Localization：Cytoplasm; Membrane; Peripheral membrane protein; Nucleus

Expression：
Tissue_specificity:In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 80% of tested tumors (at protein level)

Isoforms & Post-Translational Modification：Q6ZVD8 has 3 isomers: Q6ZVD8-1: 146751 Da (predicted); Q6ZVD8-2: 107079 Da (predicted); Q6ZVD8-3: 139394 Da (predicted).

Subunit：Interacts with AKT1, AKT3 and PRKCB isoform beta-II (PubMed:17386267, PubMed:18162466, PubMed:19732725). Interacts with STK4, RPS6KB1, RAF1 (PubMed:20513427, PubMed:21986499, PubMed:24530606). Interacts with FKBP5; FKBP5 acts as a scaffold for PHLPP2 and Akt (PubMed:19732725). Interacts with NHERF1; NHERF1 scaffolds a heterotrimeric complex with PTEN (PubMed:21804599)

Research Field

Signal Transduction

Synonyms

PHLPP2; KIAA0931, PHLPPL; PH domain leucine-rich repeat-containing protein phosphatase 2; PH domain leucine-rich repeat-containing protein phosphatase-like (PHLPP-like)

Documentation

User Guide for Antibodies (1077 KB)