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SCF Antibody (YA3875)(PBS only)

(Synonyms: SF; MGF; SCF; SLF; DCUA; FPH2; FPHH; KL-1; Kitl; SHEP7; DFNA69)

SCF Antibody (YA3875) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to SCF.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2b

  • Application:

    IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
IHC-P
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • SCF Antibody (YA3875)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

SCF Antibody (YA3875) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to SCF.

  • Host Mouse
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 18 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 31 kDa
Immunogen

Purified recombinant fragment of human KITLG (AA:Extra(26-214)) expressed in Mammalian.

Purification

affinity purified.

Conjugation

Non-conjugated

Isotype

IgG2b

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
    Immunohistochemical analysis of paraffin-embedded human Lung tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:3000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
    Immunohistochemical analysis of paraffin-embedded human Lung tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:3000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
    Immunohistochemical analysis of paraffin-embedded human Lung tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:3000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
    Immunohistochemical analysis of paraffin-embedded human spinal cord‌ tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:5000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
    Immunohistochemical analysis of paraffin-embedded human spinal cord‌ tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:5000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
    Immunohistochemical analysis of paraffin-embedded human spinal cord tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:5000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
    Immunohistochemical analysis of paraffin-embedded human Colon tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:5000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
    Immunohistochemical analysis of paraffin-embedded human Colon tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:5000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
    Immunohistochemical analysis of paraffin-embedded human Colon tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:5000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

Background

  • Function

    SCF is a Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Cytoplasm; Cytoplasm, cytoskeleton; Cell membrane; Single-pass type I membrane protein; Cell projection, lamellipodium; Cell projection, filopodium; Secreted

  • Isoforms & Post-Translational Modification

    P21583 has 3 isomers: P21583-1: 30899 Da (predicted); P21583-2: 27867 Da (predicted); P21583-3: 26667 Da (predicted).
    A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain;Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97;A soluble form exists as a cleavage product of the extracellular domain

  • Subunit

    Homodimer, non-covalently linked (Probable). Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation

  • SwissProt ID

    P21583

  • Gene ID
    4254 [NCBI]
  • Synonyms

    SF; MGF; SCF; SLF; DCUA; FPH2; FPHH; KL-1; Kitl; SHEP7; DFNA69

SCF Antibody (YA3875)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:3000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:3000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:3000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spinal cord‌ tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:5000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spinal cord‌ tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:5000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spinal cord tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:5000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:5000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:5000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:5000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SCF Antibody (YA3875)(PBS only) Product Image
    1/16
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • SCF Antibody (YA3875)(PBS only) Product Image
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:3000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:3000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:3000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spinal cord‌ tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:5000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spinal cord‌ tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:5000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spinal cord tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:5000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:5000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:5000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:5000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • Experimental Validation Results for SCF Antibody (YA3875)(PBS only)
  • SCF Antibody (YA3875)(PBS only) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Lung tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:3000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SCF Antibody (YA3875)(PBS only) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Lung tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:3000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SCF Antibody (YA3875)(PBS only) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Lung tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:3000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SCF Antibody (YA3875)(PBS only) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human spinal cord‌ tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:5000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SCF Antibody (YA3875)(PBS only) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human spinal cord‌ tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:5000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SCF Antibody (YA3875)(PBS only) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human spinal cord tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:5000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SCF Antibody (YA3875)(PBS only) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Colon tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:5000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SCF Antibody (YA3875)(PBS only) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Colon tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:5000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SCF Antibody (YA3875)(PBS only) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Colon tissue using SCF antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84178, 1:5000 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SCF Antibody (YA3875)(PBS only) Product Image IHC-P
  • SCF Antibody (YA3875)(PBS only) Product Image IHC-P
  • SCF Antibody (YA3875)(PBS only) Product Image IHC-P
  • SCF Antibody (YA3875)(PBS only) Product Image IHC-P
  • SCF Antibody (YA3875)(PBS only) Product Image IHC-P
  • SCF Antibody (YA3875)(PBS only) Product Image IHC-P
  • SCF Antibody (YA3875)(PBS only) Product Image IHC-P
  • SCF Antibody (YA3875)(PBS only) Product Image IHC-P
  • SCF Antibody (YA3875)(PBS only) Product Image IHC-P