SF3B3 Antibody (YA1171)

製品番号: HY-P81426: データシート User Guide for Antibodies
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SF3B3 Antibody (YA1171) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SF3B3.

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容量	価格（税別）	在庫状況	数量
20 μL	$146	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	$222	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	$360	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

Synthetic products have potential research and development risk.

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* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
説明

製品説明

SF3B3 Antibody (YA1171) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SF3B3.

Host

Rabbit

Clonality

Recombinant, Monoclonal

分子量

Predicted band size: 136 kDa;

Observed band size: 136 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q15393

Gene ID

23450 [NCBI]

Immunogen

A synthesized peptide derived from human SF3B3

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200

Sensitivity	Endogenous	純度	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.194.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Background

Function：Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed:10490618, PubMed:10882114, PubMed:12234937, PubMed:27720643, PubMed:28781166, PubMed:32494006, PubMed:34822310). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed:12234937, PubMed:32494006, PubMed:34822310). Within the 17S U2 SnRNP complex, SF3B3 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA (PubMed:12234937, PubMed:27720643). Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937). May also be involved in the assembly of the 'E' complex (PubMed:10882114). Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs (PubMed:15146077, PubMed:33509932)

Subcellular Localization：Nucleus

Isoforms & Post-Translational Modification：Q15393 has 3 isomers: Q15393-1: 135577 Da (predicted); Q15393-2: 30210 Da (predicted); Q15393-3: 44606 Da (predicted).

Subunit：Component of the 17S U2 SnRNP complex, a ribonucleoprotein complex that contains small nuclear RNA (snRNA) U2 and a number of specific proteins (PubMed:11991638, PubMed:12234937, PubMed:15146077, PubMed:32494006, PubMed:34822310, PubMed:36797247). Part of the SF3B subcomplex of the 17S U2 SnRNP complex (PubMed:12234937, PubMed:12738865, PubMed:27720643, PubMed:28541300). SF3B associates with the splicing subcomplex SF3A and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP) (PubMed:12234937). Within the SF3B subcomplex, interacts directly with SF3B1 (via HEAT domain), SF3B5 and PHF5A (PubMed:27720643). Identified in the spliceosome A complex; remains associated with the spliceosome throughout the splicing process (PubMed:10490618). Component of the spliceosome B complex (PubMed:28781166). Identified in the spliceosome C complex (PubMed:11991638). Identified in the spliceosome E complex (PubMed:10882114). Component of the minor (U12-type spliceosome) spliceosome (PubMed:15146077, PubMed:33509932). Within this complex, interacts with SCNM1 (PubMed:33509932). Associates with the STAGA transcription coactivator-HAT complex (PubMed:11564863). Interacts with SUPT3H (PubMed:11564863). Interacts with TAF3 (PubMed:11438666)

Database

Entrez Gene: 23450 Human

SwissProt: Q15393 Human

OMIM: 605592 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

SF3B3; KIAA0017; SAP130; Splicing factor 3B subunit 3; Pre-mRNA-splicing factor SF3b 130 kDa subunit; SF3b130; STAF130; Spliceosome-associated protein 130; SAP 130

ドキュメンテーション

User Guide for Antibodies (1077 KB)