TMEM119 Antibody (YA10102)
(Synonyms: OBIF)TMEM119 Antibody (YA10102) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TMEM119.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, IP, FC, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclin950.
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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IP
IP: Immunoprecipitation
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|---|
| Dilution Ratio | 1:2000-4000 | 1:100-1:200 | 1:800-1:1000 | 1-2μg/sample | 1:5000-10000 |
Product Details
TMEM119 Antibody (YA10102) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TMEM119.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 35/55 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 29 kDa
Purified recombinant fragment of human TMEM119 (AA: 110-280) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclin950.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
TMEM119 forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. May be involved in the control of cellular proliferation and/or differentiation. In association with ZFHX3, up-regulates CDKN1A promoter activity following TGF-beta stimulation. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing. Necessary for the development and survival of sensory neurons expressing parvalbumin[1].
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Subcellular Localization
Nucleus; Cytoplasm
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Expression
Tissue_specificity:Expression (protein level) in gastric cancer tissue -
Isoforms & Post-Translational Modification
TMEM119 has 2 isomers: Q13761-1: 44356 Da (predicted); Q13761-2: 45922 Da (predicted).
Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN -
Subunit
Heterodimer with CBFB. Interacts with TLE1 and SUV39H1.
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SwissProt ID
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Synonyms
OBIF
Documentation
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