TRIM22 Antibody (YA8595)

Cat. No.: HY-P88911: Data Sheet User Guide for Antibodies
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TRIM22 Antibody (YA8595) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TRIM22.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

TRIM22 Antibody (YA8595) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TRIM22.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 56.8 kDa

Species Reactivity

Human

SwissProt ID

Q8IYM9

Immunogen

Human recombinant protein fragment corresponding to amino acids 61-406 of human TRIM22 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Interferon-induced E3 ubiquitin ligase that plays important roles in innate and adaptive immunity (PubMed:25683609, PubMed:35777501). Restricts the replication of many viruses including HIV-1, encephalomyocarditis virus (EMCV), hepatitis B virus (HBV), hepatitis C virus (HCV) or Zika virus (ZIKV) (PubMed:25683609, PubMed:35777501, PubMed:36042495). Mechanistically, negatively regulates HCV replication by promoting ubiquitination and subsequent degradation of viral NS5A (PubMed:25683609). Also acts by promoting the degradation of Zika virus NS1 and NS3 proteins through proteasomal degradation (PubMed:36042495). Acts as a suppressor of basal HIV-1 LTR-driven transcription by preventing Sp1 binding to the HIV-1 promoter (PubMed:26683615). Also plays a role in antiviral immunity by co-regulating together with NT5C2 the RIGI/NF-kappa-B pathway by promoting 'Lys-63'-linked ubiquitination of RIGI, while NT5C2 is responsible for 'Lys-48'-linked ubiquitination of RIGI (PubMed:36159777). Participates in adaptive immunity by suppressing the amount of MHC class II protein in a negative feedback manner in order to limit the extent of MHC class II induction (PubMed:35777501)

Subcellular Localization：Cytoplasm,Nucleus,Nucleus speckle,Nucleus, Cajal body

Expression：
Tissue_Specificity: Strongly expressed in peripheral blood leukocytes, spleen, thymus, and ovary. Expressed at basal levels in other tissues
Induction: By interferons alpha and beta (PubMed:25683609). Up-regulated by p53/TP53. Dramatically induced by progesterone in MDA-MB-231-derived ABC28 cells and T47D cells. By interferon gamma (PubMed:35777501). Expression is also modulated in response to several viruses and viral antigens (PubMed:36042495)

Isoforms & Post-Translational Modification：Q8IYM9 has two isomers: Q8IYM9-1: 56947 Da (predicted); Q8IYM9-2: 56429 Da (predicted).
Auto-ubiquitinated

Subunit：Homotrimer (PubMed:17156811)

Synonyms

GPSTAF50; RNF94; STAF50

Documentation

User Guide for Antibodies (1077 KB)