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UCP3 Antibody (YA4106)

(Synonyms: SLC25A9)
Customer Review

Based on 1 Customer Validation

UCP3 Antibody (YA4106) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to UCP3.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2a

  • Application:

    ICC/IF, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for UCP3 Antibody (YA4106)
  • UCP3 Antibody (YA4106) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:10000

Product Details

Description

UCP3 Antibody (YA4106) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to UCP3.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 34 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 34 kDa
Immunogen

Purified recombinant fragment of human UCP3 (AA: 1-113 and 217-312) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2a

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for UCP3 Antibody (YA4106)
    Immunofluorescence analysis of Hela cells labeling UCP3 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with UCP3 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. AF 488 Goat Anti-mouse IgG H&L (green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

Background

  • Function

    UCP3 is a Putative transmembrane transporter that plays a role in mitochondrial metabolism via an as yet unclear mechanism. Originally, this mitochondrial protein was thought to act as a proton transmembrane transporter from the mitochondrial intermembrane space into the matrix, causing proton leaks through the inner mitochondrial membrane, thereby uncoupling mitochondrial membrane potential generation from ATP synthesis. However, this function is controversial and uncoupling may not be the function, or at least not the main function, but rather a consequence of more conventional metabolite transporter activity[1][2][3][4][5][6][7].

  • Subcellular Localization

    Mitochondrion inner membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Only in skeletal muscle and heart (PubMed:9305858) . Also expressed in white and brown adipose tissues (PubMed:9305858) . Is more expressed in glycolytic than in oxidative skeletal muscles

    Induction:Up-regulated by beta3-adrenergic stimulation, starvation, glucocorticoids and leptin

  • Isoforms & Post-Translational Modification

    P55916 has 3 isomers: P55916-1: 34216 Da (predicted); P55916-2: 29783 Da (predicted); P55916-3: 22874 Da (predicted).

  • Subunit

    Interacts with HAX1; the interaction is direct and calcium-dependent

  • SwissProt ID

    P55916

  • Gene ID
    7352 [NCBI]
  • Synonyms

    SLC25A9

UCP3 Antibody (YA4106) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunofluorescence analysis of Hela cells labeling UCP3 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with UCP3 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. AF 488 Goat Anti-mouse IgG H&L (green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

  • UCP3 Antibody (YA4106) Product Image
    1/16
  • Experimental Validation Results for UCP3 Antibody (YA4106)
  • UCP3 Antibody (YA4106) Product Image
  • Experimental Validation Results for UCP3 Antibody (YA4106)
    1/1
    MedChemExpress Validation
     Immunofluorescence analysis of Hela cells labeling UCP3 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with UCP3 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. AF 488 Goat Anti-mouse IgG H&L (green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)
  • Experimental Validation Results for UCP3 Antibody (YA4106)
  • UCP3 Antibody (YA4106) Product Image
    MedChemExpress Validation

    Immunofluorescence analysis of Hela cells labeling UCP3 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature.Cells were then incubated with UCP3 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. AF 488 Goat Anti-mouse IgG H&L (green) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

  • UCP3 Antibody (YA4106) Product Image ICC