Art. -Nr.: HY-124729

BL-918

4 Cited Publications

Kundenbewertung

Based on 4 publication(s) in Google Scholar

BL-918 is an orally active UNC-51-like kinase 1 (ULK1) activator with an EC₅₀ of 24.14 nM. BL-918 exerts its cytoprotective autophagic effect by targeting ULK complex. BL-918 has the potential for Parkinson’s disease (PD) treatment.

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Reinheit: 99.72%
CAS. Nr.: 2101517-69-3
Formel: C₂₃H₁₅F₈N₃OS
Molecular Weight:533.44

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

BL-918 Chemical Structure

CAS. Nr.: 2101517-69-3

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target^[1]

ULK1

24.14 nM (EC₅₀)

ULK1

0.719 μM (Kd)

Alle ULK Isoform-spezifische Produkte anzeigen :

ULK1
ULK2

In Vitro

BL-918 (compound 33i) binds to ULK1 with a high binding affinity (K_D=0.719 μM)^[1].
BL-918 (5 μM; for 24 hours) induces autophagy in Neuron-Like SH-SY5Y cells^[1].
BL-918 (0.5-50 μM; for 24 hours) can partially reverse MPP⁺-induced cell death, which is determined by enhancing cell viability^[1].
BL-918 (5 μM; for 6-36 hours) time-dependently elevates the expression levels of LC3-II, Beclin-1, and its phosphorylation status, whereas reduces the level of the selective autophagy substrate SQSTM1/p62. BL-918 elevates Ser317 and Ser555 phosphorylation of ULK1, as well as decreases Ser757 phosphorylation of ULK1^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Autophagy Assay^[1]

Cell Line:

SH-SY5Y cells
Concentration:

5 μM
Incubation Time:

For 24 hours
Result:

Induced Autophagy.

Cell Viability Assay^[1]

Cell Line:

SH-SY5Y cells
Concentration:

0.5, 5, 50 μM
Incubation Time:

For 24 hours
Result:

Could partially reverse MPP⁺-induced cell death, which was determined by enhancing cell viability.

Western Blot Analysis^[1]

Cell Line:

SH-SY5Y cells
Concentration:

5 μM
Incubation Time:

6, 12, 24, 36 hours
Result:

Time-dependently elevated the expression levels of LC3-II (a key marker of autophagy), Beclin-1, and its phosphorylation status, whereas reduced the level of the selective autophagy substrate SQSTM1/p62.

In Vivo

BL-918 (compound 33i; 20, 40, or 80 mg/kg/day; oral gavage; began 2 days before the first injection of saline/MPTP and continuously maintained for 5 days after the last injection of saline/MPTP) attenuates the loss of DA and its metabolites. BL-918 obviously decreases the levels of dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (HVA)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male C57BL/6 mice (eight-week old) weighing between 20 and 25 g^[1]
Dosage:

20, 40, or 80 mg/kg
Administration:

Oral gavage; daily; began 2 days before the first injection of saline/MPTP and continuously maintained for 5 days after the last injection of saline/MPTP
Result:

Attenuated the loss of DA and its metabolites.

Chemical Information

CAS. Nr. 2101517-69-3
Appearance Solid
Molecular Weight 533.44
Formel C₂₃H₁₅F₈N₃OS
Color White to off-white
SMILES

O=C(NC1=CC=C(F)C=C1F)[C@H](NC(NC2=CC(C(F)(F)F)=CC(C(F)(F)F)=C2)=S)C3=CC=CC=C3
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (4)

All Cell Proliferation/Viability AssayRT-PCRWBCell Imaging/Staining

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Journal Impact Factor

J Exp Clin Cancer Res

Ferrodoxin 1 (FDX1) drives paclitaxel resistance in ovarian cancer via copper metabolism and ULK1/ATG13-mediated autophagy: overcome by pH/ROS-responsive PPD/PDP@si-FDX1 nanomicelles. [Abstract]

2026 Apr 23;45(1):104. PMID: 42026685
Stem Cell Res Ther

Nano-zinc oxide (nZnO) targets the AMPK-ULK1 pathway to promote bone regeneration. [Abstract]

2025 Apr 24;16(1):206. PMID: 40275329

BL-918 purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2025 Apr 24;16(1):206. [Abstract]

Western blot analysis of AMPK-ULK1 and autophagic activation in MSCs after osteogenic differentiation for 72 h treated with ULK1 inhibitor SBI-0206965 (SBI, 1 µM) or agonist BL-918 (BL, 10 µM) under control and nZnO (5 µg/mL) conditions.

BL-918 purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2025 Apr 24;16(1):206. [Abstract]

ALP staining and ARS staining of MSCs after osteogenic differentiation for 7 days and 14 days treated with ULK1 inhibitor SBI or agonist BL-918 (BL) under control and nZnO (5 µg/mL) conditions, respectively.
Cell Biol Toxicol

HUWE1 regulates mitophagy to protect dopaminergic neurons from 6-OHDA- and MPP⁺-induced neurotoxicity. [Abstract]

2026 Feb 5;42(1):34. PMID: 41639490

BL-918 purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2026 Feb 5;42(1):34. [Abstract]

SH-SY5Y cells, transfected with negative control or HUWE1 siRNA, were treated with 50 μM 6-OHDA or 1 mM MPP⁺ for 24 h in the presence or absence of 20 μM BL-918. Cell viability measured by CCK-8 assay.

BL-918 purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2026 Feb 5;42(1):34. [Abstract]

SH-SY5Y cells, transfected with control or HUWE1 siRNA, were treated with 50 μM 6-OHDA or 1 mM MPP⁺ for 24 h in the presence of 20 μM BL-918. qRT-PCR analysis of HUWE1, SIRT1, PPARGC1A, NFE2L2, and TFAM mRNA expression.
Aquac Nutr

Benfotiamine, a Lipid-Soluble Derivative of Vitamin B1, Ameliorates the Carbohydrate Overload-Induced Mitochondrial Dysfunction in Fish Megalobrama amblycephala by Triggering the ULK1-Mediated Mitophagy. [Abstract]

2025 May 1:2025:7688386. PMID: 40343082

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : ≥ 250 mg/mL (468.66 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.8746 mL

9.3731 mL

18.7463 mL

5 mM

0.3749 mL

1.8746 mL

3.7492 mL

10 mM

0.1875 mL

0.9373 mL

1.8746 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (3.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Ouyang L, et al. Small-Molecule Activator of UNC-51-Like Kinase 1 (ULK1) That Induces Cytoprotective Autophagy for Parkinson's Disease Treatment. J Med Chem. 2018 Apr 12;61(7):2776-2792. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.8746 mL	9.3731 mL	18.7463 mL	46.8656 mL
	5 mM	0.3749 mL	1.8746 mL	3.7493 mL	9.3731 mL
	10 mM	0.1875 mL	0.9373 mL	1.8746 mL	4.6866 mL
	15 mM	0.1250 mL	0.6249 mL	1.2498 mL	3.1244 mL
	20 mM	0.0937 mL	0.4687 mL	0.9373 mL	2.3433 mL
	25 mM	0.0750 mL	0.3749 mL	0.7499 mL	1.8746 mL
	30 mM	0.0625 mL	0.3124 mL	0.6249 mL	1.5622 mL
	40 mM	0.0469 mL	0.2343 mL	0.4687 mL	1.1716 mL
	50 mM	0.0375 mL	0.1875 mL	0.3749 mL	0.9373 mL
	60 mM	0.0312 mL	0.1562 mL	0.3124 mL	0.7811 mL
	80 mM	0.0234 mL	0.1172 mL	0.2343 mL	0.5858 mL
	100 mM	0.0187 mL	0.0937 mL	0.1875 mL	0.4687 mL

Keywords

BL-918

4 Cited Publications

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BL-918

Autophagy Compound Library

Orally Active Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Targeted Diversity Library

Highly Selective Activators Library

Cell Death Library

Multi-Target Compound Library

Mitophagy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Protein Kinase Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) BL-918

Alle ULK Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (4)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

BL-918

Autophagy Compound Library

Orally Active Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Targeted Diversity Library

Highly Selective Activators Library

Cell Death Library

Multi-Target Compound Library

Mitophagy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Protein Kinase Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) BL-918

Alle ULK Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (4)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

BL-918 Related Classifications

HY-124729 Related Classifications