bpV(phen) trihydrate

Name: bpV(phen) trihydrate
Brand: MedChemExpress
SKU: HY-122818
Rating: 4.5 (2 reviews)

Cat. No.: HY-122818 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

bpV(phen) trihydrate, a insulin-mimetic agent, is a potent protein tyrosine phosphatase (PTP) and PTEN inhibitor with IC₅₀s of 38 nM, 343 nM and 920 nM for PTEN, PTP-β and PTP-1B, respectively. bpV(phen) trihydrate inhibits proliferation of the protozoan parasite Leishmania in vitro. bpV(phen) trihydrate strongly induces the secretion of a large number of chemokines and pro-inflammatory cytokines, and it activates a Th1-type pathway (IL-12, IFNγ). bpV(phen) trihydrate can also induce cell apoptosis, and has anti-angiogenic and anti-tumor activity.

For research use only. We do not sell to patients.

bpV(phen) trihydrate Chemical Structure

CAS No. : 171202-16-7

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in Water ready for reconstitution	USD 71	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	USD 71	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 65	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 105	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 195	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 310	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 480	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of bpV(phen) trihydrate:

bpV(phen) Get quote

2 Publications Citing Use of MCE bpV(phen) trihydrate

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•Related Small Molecules:

•Related Proteins:

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Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC50: 38 nM (PTEN), 343 nM (PTP-β) and 920 nM (PTP-1B)^[3]
Parasite Leishmania^[2]
Apoptosis^[1]

In Vitro

bpV(phen) (5 μM; 24.5 hours; H9c2 cells) treatment causes a further decrease of cell viability in H/R-injured H9c2 cells^[1].
bpV(phen) (5 μM; 24.5 hours; H9c2 cells) treatment increases the apoptosis of H/R-injured H9c2 cells^[1].
bpV(phen) (5 μM; 24.5 hours; H9c2 cells) treatment significantly promotes the accumulation of cytoplasmic Cytochrome C in H/R-injured H9c2 cells^[1].
After stimulation of bpV(phen), PTEN-induced putative kinase protein 1 (PINK1)/Parkin-mediated mitophagy is inhibited^[1].
bpV(phen) is an insulin-mimetic agent following insulin-receptor tyrosine kinase hyperphosphorylation and activation^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Hypoxia/reoxygenation (H/R)-injured H9c2 cells
Concentration:	5 μM
Incubation Time:	24.5 hours (hypoxia for 24 h; reoxygenation for 30 minutes)
Result:	Caused a further decrease of cell viability.

Apoptosis Analysis^[1]

Cell Line:	Hypoxia/reoxygenation (H/R)-injured H9c2 cells
Concentration:	5 μM
Incubation Time:	24.5 hours (hypoxia for 24 h; reoxygenation for 30 minutes)
Result:	Increased the apoptosis of H/R-injured H9c2 cells.

Western Blot Analysis^[1]

Cell Line:	Hypoxia/reoxygenation (H/R)-injured H9c2 cells
Concentration:	5 μM
Incubation Time:	24.5 hours (hypoxia for 24 h; reoxygenation for 30 minutes)
Result:	Showed an increased release of Cytochrome C.

In Vivo

bpV(phen) (5 mg/kg; intraperitoneal injection; daily; for 38 days; male BALB/c nude (nu/nu) athymic mice) treatment causes a significant reduction in average tumor volume^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male BALB/c nude (nu/nu) athymic mice (6-7 weeks old) injected with PC-3 cells^[2]
Dosage:	5 mg/kg
Administration:	Intraperitoneal injection; daily; for 38 days
Result:	Caused a significant reduction in average tumor volume.

Molecular Weight

404.29

Formula

C₁₂H₁₄KN₂O₈V

CAS No.

171202-16-7

Appearance

Solid

Color

Yellow to orange

SMILES

O=[V+3]12([N]3=CC=C4)([O-][O-]1)([O-][O-]2)[N]5=C6C3=C4C=CC6=CC=C5.O.O.O.[K+]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 18.18 mg/mL (44.97 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4735 mL	12.3674 mL	24.7347 mL
5 mM	0.4947 mL	2.4735 mL	4.9469 mL
10 mM	0.2473 mL	1.2367 mL	2.4735 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 25 mg/mL (61.84 mM); Clear solution; Need ultrasonic and warming

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (299 KB) SDS (252 KB)

COA (260 KB) HNMR (258 KB)

Handling Instructions (2659 KB)

References

[1]. Tang W, et al. PTEN-mediated mitophagy and APE1 overexpression protects against cardiac hypoxia/reoxygenation injury. In Vitro Cell Dev Biol Anim. 2019 Oct;55(9):741-748. [Content Brief]

[2]. Caron D, et al. Protein tyrosine phosphatase inhibition induces anti-tumor activity: evidence of Cdk2/p27 kip1 and Cdk2/SHP-1 complex formation in human ovarian cancer cells. Cancer Lett. 2008 Apr 18;262(2):265-75. [Content Brief]

[3]. Schmid AC, et al. Bisperoxovanadium compounds are potent PTEN inhibitors. FEBS Lett. 2004 May 21;566(1-3):35-8. [Content Brief]

[4]. Band CJ, et al. Early signaling events triggered by peroxovanadium [bpV(phen)] are insulin receptor kinase (IRK)-dependent: specificity of inhibition of IRK-associated protein tyrosine phosphatase(s) by bpV(phen). Mol Endocrinol. 1997 Dec;11(13):1899-910. [Content Brief]

[5]. Chen Q, et al. Potassium Bisperoxo(1,10-phenanthroline)oxovanadate (bpV(phen)) Induces Apoptosis and Pyroptosis and Disrupts the P62-HDAC6 Protein Interaction to Suppress the Acetylated Microtubule-dependent Degradation of Autophagosomes. J Biol Chem. 201 [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	2.4735 mL	12.3674 mL	24.7347 mL	61.8368 mL
	5 mM	0.4947 mL	2.4735 mL	4.9469 mL	12.3674 mL
	10 mM	0.2473 mL	1.2367 mL	2.4735 mL	6.1837 mL
	15 mM	0.1649 mL	0.8245 mL	1.6490 mL	4.1225 mL
	20 mM	0.1237 mL	0.6184 mL	1.2367 mL	3.0918 mL
	25 mM	0.0989 mL	0.4947 mL	0.9894 mL	2.4735 mL
	30 mM	0.0824 mL	0.4122 mL	0.8245 mL	2.0612 mL
	40 mM	0.0618 mL	0.3092 mL	0.6184 mL	1.5459 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.