2. Metabolic Enzyme/Protease
  3. Phosphatase
  4. DiFMUP

DiFMUP  (Synonyms: 6,8-Difluoro-4-methylumbelliferyl phosphate)

Cat. No.: HY-120166 Purity: 99.64%
COA Handling Instructions

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DiFMUP is a fluorogenic substrate, and has been widely used for the continuous detection of phosphatase activities. DiFMUP is hydrolysis by a phosphatase results in the release of Xuorescent DIFMU, which can be easily followed in continuous mode by a Xuorescence reader.

For research use only. We do not sell to patients.

DiFMUP Chemical Structure

DiFMUP Chemical Structure

CAS No. : 214491-43-7

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 154 In-stock
Solution
10 mM * 1 mL in DMSO USD 154 In-stock
Solid
5 mg USD 140 In-stock
10 mg USD 230 In-stock
25 mg USD 460 In-stock
50 mg USD 740 In-stock
100 mg USD 1150 In-stock
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Based on 1 publication(s) in Google Scholar

DiFMUP Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

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Description

DiFMUP is a fluorogenic substrate, and has been widely used for the continuous detection of phosphatase activities. DiFMUP is hydrolysis by a phosphatase results in the release of Xuorescent DIFMU, which can be easily followed in continuous mode by a Xuorescence reader[1][2].

In Vitro

DIFMUP (100 μM; 0-4 min) is hydrolyzed by protein tyrosine phosphatase 1B (PTP1B) in a time-dependent manner and the initial rate of the reaction velocity is increased with the enzyme concentration (30-600 ng/mL)[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DiFMUP Related Antibodies
Molecular Weight

292.13

Formula

C10H7F2O6P
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=P(OC1=C(C(OC2=O)=C(C(C)=C2)C=C1F)F)(O)O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (171.16 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4231 mL 17.1157 mL 34.2313 mL
5 mM 0.6846 mL 3.4231 mL 6.8463 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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mg/kg

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g

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.4231 mL 17.1157 mL 34.2313 mL 85.5783 mL
5 mM 0.6846 mL 3.4231 mL 6.8463 mL 17.1157 mL
10 mM 0.3423 mL 1.7116 mL 3.4231 mL 8.5578 mL
15 mM 0.2282 mL 1.1410 mL 2.2821 mL 5.7052 mL
20 mM 0.1712 mL 0.8558 mL 1.7116 mL 4.2789 mL
25 mM 0.1369 mL 0.6846 mL 1.3693 mL 3.4231 mL
30 mM 0.1141 mL 0.5705 mL 1.1410 mL 2.8526 mL
40 mM 0.0856 mL 0.4279 mL 0.8558 mL 2.1395 mL
50 mM 0.0685 mL 0.3423 mL 0.6846 mL 1.7116 mL
60 mM 0.0571 mL 0.2853 mL 0.5705 mL 1.4263 mL
80 mM 0.0428 mL 0.2139 mL 0.4279 mL 1.0697 mL
100 mM 0.0342 mL 0.1712 mL 0.3423 mL 0.8558 mL
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DiFMUP Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DiFMUP214491-43-76,8-Difluoro-4-methylumbelliferylPhosphatasefluorogenicsubstratephosphataseactivitiesDIFMUInhibitorinhibitorinhibit

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