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DiFMUP (Synonyms: 6,8-Difluoro-4-methylumbelliferyl phosphate)

Cat. No.: HY-120166
Handling Instructions

DiFMUP is a fluorogenic substrate, and has been widely used for the continuous detection of phosphatase activities. DiFMUP is hydrolysis by a phosphatase results in the release of Xuorescent DIFMU, which can be easily followed in continuous mode by a Xuorescence reader.

For research use only. We do not sell to patients.

DiFMUP Chemical Structure

DiFMUP Chemical Structure

CAS No. : 214491-43-7

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Description

DiFMUP is a fluorogenic substrate, and has been widely used for the continuous detection of phosphatase activities. DiFMUP is hydrolysis by a phosphatase results in the release of Xuorescent DIFMU, which can be easily followed in continuous mode by a Xuorescence reader[1][2].

In Vitro

DIFMUP (100 μM; 0-4 min) is hydrolyzed by protein tyrosine phosphatase 1B (PTP1B) in a time-dependent manner and the initial rate of the reaction velocity is increased with the enzyme concentration (30-600 ng/mL)[2].

Molecular Weight

292.13

Formula

C₁₀H₇F₂O₆P

CAS No.

214491-43-7

SMILES

O=P(OC1=C(C(OC2=O)=C(C(C)=C2)C=C1F)F)(O)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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Keywords:

DiFMUP6,8-Difluoro-4-methylumbelliferylOthersfluorogenicsubstratephosphataseactivitiesDIFMUInhibitorinhibitorinhibit

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