CX3CL1 - C-X3-C motif chemokine ligand 1 Gene

Also Known as NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 6376

About CX3CL1

Cytogenetic location: 16q21 Genomic coordinates (GRCh38): 16:57,372,490-57,385,044 (from NCBI)

This gene has 4 transcripts (splice variants), 155 orthologues and 25 paralogues. Broad expression in brain (RPKM 40.1), lung (RPKM 37.8) and 21 other tissues.

Summary

This gene belongs to the CX3C subgroup of chemokines, characterized by the number of Amino acids located between the conserved cysteine residues. This is the only member of the CX3C subgroup, which contains three Amino acids between cysteine residues, resulting in a Cys-X-X-X-Cys configuration. The encoded protein contains an extended mucin-like stalk with a chemokine domain on top, and exists in both a membrane-anchored form where it acts as a binding molecule, or, in soluble form, as a chemotactic cytokine. The mature form of this protein can be cleaved at the cell surface, yielding different soluble forms that can interact with the G-protein coupled receptor, C-X3-C motif Chemokine Receptor 1 gene product. This gene plays a role in a wide range of diseases, including Cancer, vasculitis, neuropathies, atherosclerosis, inflammatory diseases, and in human immunodeficiency virus infections. [provided by RefSeq, Sep 2017]

CX3CL1 Products (2)

mRNA	Protein	Name
NM_001304392.3	NP_001291321.1	fractalkine isoform 2
NM_002996.6	NP_002987.1	fractalkine isoform 1 precursor

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables CX3C chemokine receptor binding	IDA IDA: Inferred from direct assay	10415068	GOA
enables CXCR1 chemokine receptor binding	IPI IPI: Inferred from physical interaction	10187784	GOA
enables chemoattractant activity	IMP IMP: Inferred from mutant phenotype	10899174	GOA
enables chemokine activity	IDA IDA: Inferred from direct assay	21829356	GOA
enables chemokine activity	IGI IGI: Inferred from genetic interaction	10415068	GOA
enables integrin binding	IDA IDA: Inferred from direct assay	23125415	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	9931005	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in cell adhesion	IPI IPI: Inferred from physical interaction	10187784	GOA
involved in cell chemotaxis	IDA IDA: Inferred from direct assay	10415068	GOA
involved in chemokine-mediated signaling pathway	IDA IDA: Inferred from direct assay	10415068	GOA
involved in chemokine-mediated signaling pathway	IGI IGI: Inferred from genetic interaction	10869418	GOA
involved in chemotaxis	IDA IDA: Inferred from direct assay	21829356	GOA
involved in chemotaxis	IMP IMP: Inferred from mutant phenotype	23125415	GOA
involved in integrin activation	IMP IMP: Inferred from mutant phenotype	23125415	GOA
involved in leukocyte migration involved in inflammatory response	IMP IMP: Inferred from mutant phenotype	23125415	GOA
involved in microglial cell proliferation	IMP IMP: Inferred from mutant phenotype	12125082	GOA
involved in negative regulation of apoptotic process	IMP IMP: Inferred from mutant phenotype	24294368	GOA
involved in negative regulation of apoptotic signaling pathway	IGI IGI: Inferred from genetic interaction	10869418	GOA
involved in negative regulation of cell migration	IDA IDA: Inferred from direct assay	25435433	GOA
involved in negative regulation of hippocampal neuron apoptotic process	IGI IGI: Inferred from genetic interaction	10869418	GOA
involved in negative regulation of tumor necrosis factor production	IDA IDA: Inferred from direct assay	10652441	GOA
involved in positive chemotaxis	IMP IMP: Inferred from mutant phenotype	10899174	GOA
involved in positive regulation of I-kappaB phosphorylation	IGI IGI: Inferred from genetic interaction	10869418	GOA
involved in positive regulation of MAPK cascade	IGI IGI: Inferred from genetic interaction	10415068	GOA
involved in positive regulation of actin filament bundle assembly	IGI IGI: Inferred from genetic interaction	10415068	GOA
involved in positive regulation of calcium-independent cell-cell adhesion	IDA IDA: Inferred from direct assay	12714508	GOA
involved in positive regulation of canonical NF-kappaB signal transduction	IGI IGI: Inferred from genetic interaction	10869418	GOA
involved in positive regulation of cell population proliferation	IMP IMP: Inferred from mutant phenotype	24294368	GOA
involved in positive regulation of inflammatory response	IEP IEP: Inferred from expression pattern	11777952	GOA
involved in positive regulation of microglial cell migration	IDA IDA: Inferred from direct assay	10415068	GOA
involved in positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	IGI IGI: Inferred from genetic interaction	10415068	GOA
involved in positive regulation of release of sequestered calcium ion into cytosol	IGI IGI: Inferred from genetic interaction	10415068	GOA
involved in regulation of lipopolysaccharide-mediated signaling pathway	IDA IDA: Inferred from direct assay	10652441	GOA

Cellular Component GO Annotation	Evidence	References	Source
located in cell surface	IDA IDA: Inferred from direct assay	12714508	GOA
located in extracellular region	IDA IDA: Inferred from direct assay	10415068	GOA
located in extracellular region	IMP IMP: Inferred from mutant phenotype	10899174	GOA
located in membrane	IDA IDA: Inferred from direct assay	10899174	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

CX3CL1 Protein Structure

IL8

0
100
200
300
397 a.a.

Protein Preferred Names	Protein Names
fractalkine	C-X3-C motif chemokine 1

CX3CL1 Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	CX3CL1	P78423	ITGB3	Homo sapiens	P05106	ELISA	24789099
Intra	CX3CL1	P78423	ITGB3	Homo sapiens	P05106	ELISA	23125415
Intra	CX3CL1	P78423	ITGB3	Homo sapiens	P05106	Pull Down	24789099

Cross: Cross-species interaction Intra: Intraspecies interaction

Recombinant CX3CL1 Proteins

Cat. No.	Product Name	Accession	Purity
HY-P7180	Fractalkine/CX3CL1 Protein, Human	P78423 (Q25-G100)	≥ 95%, as determined by reducing SDS-PAGE.
HY-P7355	Fractalkine/CX3CL1 Protein, Human (S199N, CHO)	P78423 (Q25-R339, S199N)	≥ 95%, as determined by reducing SDS-PAGE.
HY-P72685	Fractalkine/CX3CL1 Protein, Human (315a.a, HEK293, His)	P78423 (Q25-R339)	≥ 95%, as determined by reducing SDS-PAGE.
HY-P76938	Fractalkine/CX3CL1 Protein, Cynomolgus (HEK293, Fc)	EHH60415.1 (Q25-G100)	≥ 95%, as determined by reducing SDS-PAGE.
HY-P76939	Fractalkine/CX3CL1 Protein, Cynomolgus (HEK293, His)	EHH60415.1 (Q25-G100)	≥ 95%, as determined by reducing SDS-PAGE.
HY-P78937	Fractalkine/CX3CL1 Protein, Human (Biotinylated, HEK293, His, Avi)	P78423/AAH01163 (Q25-Q341)	≥ 95%, as determined by reducing SDS-PAGE.

CX3CL1 Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P811043	Fractalkine Antibody	WB, ICC/IF	Human, Mouse, Rat

Related Diseases

Diseases	Alias
Pityriasis Rosea	Pityriasis Circinata Gibert Pityriasis
Vasculitis	Angiitis Autoimmune Vasculitis Systemic Vasculitis Vasculitis, Autoimmune
Chorioretinitis	Retinochoroiditis
Encephalitis	Mumps Encephalitis Mumps Meningoencephalitis Herpes Simplex Neuroinvasion Herpetic Encephalitis Herpetic Encephalopathy Herpes Simplex Encephalitis, Myelitis Or Encephalomyelitis Encephalitis Due To Herpesviridae Encephalitis Due To Herpesvirus Herpes Encephalitis Herpesviral Encephalitis Herpes Simplex Encephalitis Hsv - [Herpes Simplex Virus] Encephalitis Herpes Virus Encephalitis Simian B Disease Simian B Disorder Encephalitis Nec Idiopathic Encephalitis
Neuritis	Peripheral Neuritis
Rheumatoid Arthritis	RA Arthritis, Rheumatoid Rheumatoid Arthritis, Susceptibility To Arthritis Or Polyarthritis, Rheumatic Atrophic Arthritis Rheumatism Arthritis Rheumatoid Polyarthritis
Chronic Cholangitis
Inflammatory Bowel Disease	Inflammatory Bowel Diseases Bowel Disease, Inflammatory
Retinal Degeneration	Degeneration Of Retina
Refractory Plasma Cell Neoplasm
Polycystic Lipomembranous Osteodysplasia With Sclerosing Leukoencephalopathy 1	Nasu-Hakola Disease Polycystic Lipomembranous Osteodysplasia With Sclerosing Leukoencephalopathy Plosl Nhd Presenile Dementia With Bone Cysts Plo-Sl PLOSL1 Dementia, Prefrontal, With Bone Cysts Dementia, Progressive, With Lipomembranous Polycystic Osteodysplasia Brain-Bone-Fat Disease Progressive Dementia With Lipomembranous Polycystic Osteodysplasia Brain-Bone-Fat Disease
Systemic Lupus Erythematosus	Lupus Nephritis SLE Disseminated Lupus Erythematosus Systemic Lupus Erythematosus, Susceptibility To Lupus Erythematosus, Systemic Lupus Nephritis, Susceptibility To Libman-Sacks Disease Systemic Lupus Erythematosus Susceptibility To Sle - Lupus Erythematosus, Systemic Le Syndrome Lupus Lupus Erythematosus Systemic Lupus Erythematosus, Systemic, Susceptibility To Lupus Vulgaris Lupus Erythematosus, Discoid Lupus Erythematosus Systemic Lupus Erythematosus Nos Sle - [Systemic Lupus Erythematosus]
Heart Disease	Heart Failure Congenital Heart Disease Heart Diseases Congenital Heart Defects Congenital Heart Defect Heart Malformation Congenital Anomaly Of Heart Heart Defect Heart-Congenital Defect Congenital Heart Disorder Heart Defects Congenital Heart Defects, Congenital Heart Defects Heart Disease, Congenital Disease, Heart, Congenital Congestive Heart Failure
Dermatitis, Atopic	Atopic Dermatitis Atopic Eczema Dermatitis, Atopic, Susceptibility To, 1 Atod Eczema, Atopic Dermatitis, Atopic 1 Allergic Dermatitis Atopic Neurodermatitis Besnier'S Prurigo Dermatitis, Atopic, 1 Dermatitis Atopic Eczema Besnier Prurigo
Amyotrophic Lateral Sclerosis 1	Amyotrophic Lateral Sclerosis ALS Lou Gehrig Disease Amyotrophic Lateral Sclerosis Type 1 Charcot Disease ALS1 Amyotrophic Lateral Sclerosis, Susceptibility To Fals Lou Gehrig'S Disease Mnd Motor Neuron Disease Familial Amyotrophic Lateral Sclerosis Amyotrophic Lateral Sclerosis 1, Familial Amyotrophic Lateral Sclerosis 1, Autosomal Dominant Motor Neuron Disease, Bulbar Motor Neurone Disease Amyotrophic Lateral Sclerosis With Dementia Dementia With Amyotrophic Lateral Sclerosis Motor Neuron Disease, Amyotrophic Lateral Sclerosis Sclerosis, Lateral, Amyotrophic Sclerosis, Lateral, Amyotrophic, Type 1 Amyotrophic Sclerosis Als - [Amyotrophic Lateral Sclerosis] Wasting Palsy Amyotrophic Paralysis Amyotrophy Lateral Sclerosis Wasting Paralysis Spinal Progressive Amyotrophy Progressive Atrophic Paralysis
Nervous System Disease	Abnormality Of The Nervous System Nervous System Diseases Nervous System Disorder
Retinitis Pigmentosa	RP Rod-Cone Dystrophy Autosomal Recessive Retinitis Pigmentosa Non-Syndromic Retinitis Pigmentosa Pericentral Pigmentary Retinopathy Pigmentary Retinopathy Tapetoretinal Degeneration Rcd Retinitis Pigmentosa Autosomal Recessive ARRP Retinitis Pigmentosa, Autosomal Recessive Retinitis Pigmentosa 1

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (5)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-175746	AZD0233	Antagonist	98.66%	Unkown
HY-RS16603	Cx3cl1 Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS03378	CX3CL1 Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-181016	CX3CR1-IN-2	Inhibitor	/	Unkown
HY-RS23037	Cx3cl1 Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Inhibitory Antibodies (1)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-P99404	Quetmolimab	Inhibitor	99.9%	Unkown

Orthologs Information

Species	Symbol	Source	ID
Felis catus	CX3CL1	VGNC	VGNC:61296
Mus musculus	CX3CL1	MGD	MGI:1097153
Rattus norvegicus	CX3CL1	RGD	RGD:620458
Canis familiaris	CX3CL1	VGNC	VGNC:39745
Bos taurus	CX3CL1	VGNC	VGNC:52190
Macaca mulatta	CX3CL1	VGNC	VGNC:71395
Others	CX3CL1	NCBI